Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACH41_RS09880 Genome accession   NZ_AP029023
Coordinates   2117520..2118563 (-) Length   347 a.a.
NCBI ID   WP_194748247.1    Uniprot ID   -
Organism   Methylophilus sp. DW102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2112520..2123563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH41_RS09855 (MTDW_19250) ruvX 2113007..2113510 (+) 504 WP_194748242.1 Holliday junction resolvase RuvX -
  AACH41_RS09860 (MTDW_19260) pyrR 2113523..2114056 (+) 534 WP_194748243.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AACH41_RS09865 (MTDW_19270) - 2114049..2115005 (+) 957 WP_194748244.1 aspartate carbamoyltransferase catalytic subunit -
  AACH41_RS09870 (MTDW_19280) - 2115002..2116297 (+) 1296 WP_338654835.1 dihydroorotase -
  AACH41_RS09875 (MTDW_19290) pilU 2116347..2117510 (-) 1164 WP_194748246.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AACH41_RS09880 (MTDW_19300) pilT 2117520..2118563 (-) 1044 WP_194748247.1 type IV pilus twitching motility protein PilT Machinery gene
  AACH41_RS09885 (MTDW_19310) - 2118630..2119334 (+) 705 WP_338654836.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH41_RS09890 (MTDW_19320) proC 2119353..2120183 (+) 831 WP_338654837.1 pyrroline-5-carboxylate reductase -
  AACH41_RS09895 (MTDW_19330) - 2120195..2120731 (+) 537 WP_338654838.1 YggT family protein -
  AACH41_RS09900 (MTDW_19340) rng 2120803..2122275 (-) 1473 WP_228518766.1 ribonuclease G -
  AACH41_RS09905 (MTDW_19350) - 2122316..2122882 (-) 567 WP_338657598.1 Maf family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38384.96 Da        Isoelectric Point: 6.4331

>NTDB_id=108168 AACH41_RS09880 WP_194748247.1 2117520..2118563(-) (pilT) [Methylophilus sp. DW102]
MDISDLLAFSVKNKASDLHLSAGLPPMIRVHGDVRKINLPAMDHAEVHSMLYDIMNDSQRKVYEEKLECDFSFEIPDLAR
FRVNAFNHQRGAGAVFRTIPSKVLTLEELNAPRVFKDLADTPRGIVLVTGPTGSGKSTTLAAMIDYINESQMSHILTVED
PIEFVHTSKKSLINQREVGPHTMSFENALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLCKTKDEQGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQSVGMQTLDQN
LQDLVRRNVISADEARTKAANRDGIIG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=108168 AACH41_RS09880 WP_194748247.1 2117520..2118563(-) (pilT) [Methylophilus sp. DW102]
GTGGATATTTCAGATTTATTGGCGTTTTCAGTTAAAAACAAAGCCTCAGACTTGCATTTGTCAGCCGGGTTGCCACCGAT
GATACGCGTGCATGGCGATGTGCGTAAAATCAACTTACCCGCCATGGATCATGCTGAAGTACATAGCATGTTGTACGACA
TCATGAATGACAGCCAGCGCAAAGTGTATGAAGAAAAGCTGGAATGCGATTTTTCATTCGAGATCCCTGACCTGGCGCGT
TTCCGGGTCAATGCATTTAACCACCAGCGCGGCGCCGGTGCGGTATTCCGTACCATTCCTAGCAAGGTGTTGACGCTGGA
AGAGCTTAACGCCCCACGCGTGTTCAAGGACCTGGCCGATACGCCGCGTGGCATTGTGCTGGTGACCGGGCCGACCGGTT
CAGGTAAATCGACCACACTGGCGGCGATGATTGATTACATTAATGAATCCCAGATGTCGCATATCCTGACCGTGGAAGAC
CCGATCGAATTTGTGCATACCTCGAAAAAGTCATTGATTAACCAGCGTGAAGTCGGGCCGCATACCATGTCGTTTGAAAA
TGCGCTGCGGTCAGCCTTGCGTGAAGACCCGGATGTGATTCTGGTGGGTGAAATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACTGCGGCCGAAACCGGTCACCTGGTATTTGGGACCCTGCATACCAGCTCTGCGGCCAAGACCATAGACCGGGTA
GTCGATGTGTTCCCGGCGGCAGAAAAGGAAATGGTGCGCTCCATGCTCTCAGAGTCTTTACGCGCCGTGATCTCGCAAAC
CCTGTGTAAAACCAAAGACGAGCAGGGCCGGGTGGCTGCCCATGAAATCATGATCGGAACCCCCGCCATCCGTAACCTGA
TTCGCGAAAACAAGATTGCGCAGATGTATTCGGCAATTCAGACGGGCCAAAGTGTGGGCATGCAAACGCTGGACCAGAAT
CTGCAAGATCTGGTGCGACGCAACGTCATCAGCGCCGATGAGGCGCGTACCAAAGCGGCTAACCGTGATGGCATTATCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.171

97.695

0.764
  pilT Acinetobacter baumannii D1279779

78.171

97.695

0.764
  pilT Acinetobacter baumannii strain A118

78.171

97.695

0.764
  pilT Pseudomonas aeruginosa PAK

76.401

97.695

0.746
  pilT Pseudomonas stutzeri DSM 10701

76.106

97.695

0.744
  pilT Acinetobacter baylyi ADP1

75.516

97.695

0.738
  pilT Neisseria meningitidis 8013

72.174

99.424

0.718
  pilT Neisseria gonorrhoeae MS11

71.884

99.424

0.715
  pilT Legionella pneumophila strain Lp02

71.93

98.559

0.709
  pilT Legionella pneumophila strain ERS1305867

71.93

98.559

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.406

0.66
  pilT Vibrio cholerae strain A1552

67.751

97.406

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.802

97.695

0.516
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

41.176

97.983

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment