Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH41_RS09875 Genome accession   NZ_AP029023
Coordinates   2116347..2117510 (-) Length   387 a.a.
NCBI ID   WP_194748246.1    Uniprot ID   -
Organism   Methylophilus sp. DW102     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2111347..2122510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH41_RS09850 (MTDW_19240) - 2112409..2113014 (+) 606 WP_194748241.1 YqgE/AlgH family protein -
  AACH41_RS09855 (MTDW_19250) ruvX 2113007..2113510 (+) 504 WP_194748242.1 Holliday junction resolvase RuvX -
  AACH41_RS09860 (MTDW_19260) pyrR 2113523..2114056 (+) 534 WP_194748243.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AACH41_RS09865 (MTDW_19270) - 2114049..2115005 (+) 957 WP_194748244.1 aspartate carbamoyltransferase catalytic subunit -
  AACH41_RS09870 (MTDW_19280) - 2115002..2116297 (+) 1296 WP_338654835.1 dihydroorotase -
  AACH41_RS09875 (MTDW_19290) pilU 2116347..2117510 (-) 1164 WP_194748246.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AACH41_RS09880 (MTDW_19300) pilT 2117520..2118563 (-) 1044 WP_194748247.1 type IV pilus twitching motility protein PilT Machinery gene
  AACH41_RS09885 (MTDW_19310) - 2118630..2119334 (+) 705 WP_338654836.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH41_RS09890 (MTDW_19320) proC 2119353..2120183 (+) 831 WP_338654837.1 pyrroline-5-carboxylate reductase -
  AACH41_RS09895 (MTDW_19330) - 2120195..2120731 (+) 537 WP_338654838.1 YggT family protein -
  AACH41_RS09900 (MTDW_19340) rng 2120803..2122275 (-) 1473 WP_228518766.1 ribonuclease G -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43120.43 Da        Isoelectric Point: 6.5030

>NTDB_id=108167 AACH41_RS09875 WP_194748246.1 2116347..2117510(-) (pilU) [Methylophilus sp. DW102]
MDSSMQTGADHAQTEKFINDLLKLMVTRKASDLFITAGFPPAMKIDGKLTPITSQTLSTQQTAEIARTVMNDKQAQEFKE
TMECNFAISMPSVGRFRVNAFVQRSAVGLVIRVINTNIPKFDDLNLPPVLKDVVMAKRGLIIFVGGTGSGKSTSMAAMLG
HRNESSYGHIITIEDPVEFVHSHRNCIVTQREVGVDTDNWHAALKNTLRQAPDVIMIGEIRDRETMDYAIAFSETGHLCL
ATLHANSTNQALDRIINFFPEERRQQLLMDLSLNLRCIASQRLIPKVDGSGRVAAVEVLLHSPLISDLIYKGDVHGIKEV
MAKSRELGMQTFDQALFDLFETSQISYEDALRNADSVNDLRLKFKLNSKVANPENQERSKISDLDIL

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=108167 AACH41_RS09875 WP_194748246.1 2116347..2117510(-) (pilU) [Methylophilus sp. DW102]
ATGGATAGCAGCATGCAGACCGGCGCCGATCACGCGCAAACCGAGAAATTTATTAATGACCTGTTGAAGCTCATGGTCAC
GCGTAAAGCCTCGGATTTGTTTATTACCGCAGGCTTTCCGCCGGCCATGAAGATTGATGGCAAGCTCACACCGATTACCT
CACAAACTTTGAGTACCCAGCAAACGGCCGAAATTGCGCGTACTGTCATGAATGACAAGCAAGCGCAAGAGTTTAAAGAA
ACCATGGAGTGCAATTTTGCGATCAGCATGCCATCGGTGGGCCGTTTCCGCGTCAATGCCTTTGTGCAGCGCAGTGCCGT
AGGTCTGGTGATTCGGGTGATCAATACCAATATCCCCAAATTTGATGATTTGAACTTGCCGCCCGTACTCAAGGATGTGG
TGATGGCAAAGCGCGGCCTGATTATTTTTGTTGGCGGCACGGGATCAGGTAAGTCGACCTCAATGGCCGCCATGCTGGGG
CACCGCAATGAAAGCAGTTATGGCCACATTATCACCATTGAAGACCCGGTAGAGTTTGTGCATAGCCACCGCAACTGCAT
TGTCACCCAGCGCGAAGTGGGCGTGGATACGGACAACTGGCATGCCGCGCTCAAGAACACGCTACGTCAGGCACCGGATG
TGATCATGATCGGTGAAATCCGCGACCGTGAAACCATGGATTACGCGATTGCCTTTTCTGAAACCGGCCACTTGTGTCTG
GCCACCTTGCACGCCAACAGCACCAACCAGGCGCTGGACCGCATTATCAACTTTTTTCCCGAAGAACGCCGGCAGCAATT
GCTGATGGACCTGTCGCTCAACCTGCGCTGTATTGCCTCACAACGGTTGATTCCTAAAGTGGATGGTTCTGGTCGTGTGG
CTGCCGTCGAAGTTCTGCTGCACTCGCCGCTGATTTCAGACCTGATTTATAAAGGTGATGTACACGGCATTAAAGAAGTC
ATGGCCAAATCCCGAGAGCTGGGCATGCAGACCTTTGATCAGGCCTTGTTTGACCTGTTTGAAACCAGCCAGATCAGTTA
CGAAGACGCCTTGCGCAATGCCGACTCGGTCAACGACTTGCGACTCAAATTTAAACTCAACAGCAAAGTCGCCAACCCGG
AAAATCAGGAACGCAGCAAGATTTCTGATCTGGATATTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

60.773

93.54

0.568
  pilU Pseudomonas stutzeri DSM 10701

62.464

90.181

0.563
  pilU Vibrio cholerae strain A1552

55.747

89.922

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

87.08

0.37
  pilT Pseudomonas aeruginosa PAK

41.543

87.08

0.362

Multiple sequence alignment