Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFGDV_RS23285 Genome accession   NZ_CP176528
Coordinates   5015905..5016948 (+) Length   347 a.a.
NCBI ID   WP_136418844.1    Uniprot ID   -
Organism   Noviherbaspirillum sp. ST 5-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5010905..5021948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFGDV_RS23255 ubiA 5012371..5013225 (+) 855 WP_136418850.1 4-hydroxybenzoate octaprenyltransferase -
  ACFGDV_RS23260 - 5013318..5013632 (+) 315 WP_136418849.1 YqjD family protein -
  ACFGDV_RS23265 - 5013637..5014002 (+) 366 WP_136418848.1 phage holin family protein -
  ACFGDV_RS23270 - 5013999..5014298 (+) 300 WP_136418847.1 YqjK family protein -
  ACFGDV_RS23275 proC 5014307..5015131 (-) 825 WP_136418846.1 pyrroline-5-carboxylate reductase -
  ACFGDV_RS23280 - 5015121..5015825 (-) 705 WP_136418845.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFGDV_RS23285 pilT 5015905..5016948 (+) 1044 WP_136418844.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFGDV_RS23290 pilU 5017007..5018143 (+) 1137 WP_136418843.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFGDV_RS23295 - 5018140..5018625 (+) 486 WP_136418842.1 glutathione peroxidase -
  ACFGDV_RS23300 glcF 5018902..5020134 (-) 1233 WP_136418841.1 glycolate oxidase subunit GlcF -
  ACFGDV_RS23305 glcE 5020137..5021204 (-) 1068 WP_136418840.1 glycolate oxidase subunit GlcE -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37967.73 Da        Isoelectric Point: 6.7264

>NTDB_id=1080728 ACFGDV_RS23285 WP_136418844.1 5015905..5016948(+) (pilT) [Noviherbaspirillum sp. ST 5-3]
MDISELLAFAVKNGASDLHLSAGLPPMIRVHGDVRRINLPPLEHRDVHGMIYDIMNDGQRKAYEEVLECDFSFAIPGLAR
FRVNAFNQDRGAAAVLRTIPSKVLTLEQLNAPKIFTELALKPRGLVLVTGPTGSGKSTTLAAMINHLNETEYGHILTVED
PIEFVHESKKCLINQREVGPHTMSFQNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMIRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGSNIGMQTLDQN
LTDLVRRNVISPAAARAAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1080728 ACFGDV_RS23285 WP_136418844.1 5015905..5016948(+) (pilT) [Noviherbaspirillum sp. ST 5-3]
ATGGACATTTCTGAACTTCTCGCCTTTGCCGTCAAGAACGGCGCATCCGACCTGCACCTGTCCGCCGGCTTGCCGCCGAT
GATACGCGTGCATGGCGACGTGCGTCGCATCAATTTGCCGCCGCTGGAGCACCGCGATGTGCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGCGTACGAAGAAGTGCTCGAATGCGACTTTTCGTTTGCCATTCCGGGCCTGGCGCGC
TTCCGCGTGAACGCATTCAACCAGGACCGCGGTGCGGCGGCCGTGCTGCGTACCATTCCGTCGAAAGTGCTGACGCTGGA
ACAACTGAATGCGCCGAAGATTTTCACCGAGCTGGCATTGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CCGGCAAATCGACCACACTGGCGGCGATGATCAATCATCTGAACGAAACCGAATACGGCCACATCCTGACCGTGGAAGAT
CCGATCGAATTCGTGCACGAATCGAAGAAATGCCTGATCAACCAGCGTGAAGTCGGTCCGCACACCATGTCCTTCCAGAA
CGCGCTGCGCTCCGCCCTGCGTGAAGACCCGGACGCGATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATTCGCCTTG
CGATGACGGCGGCCGAAACCGGCCACCTTGTGTTCGGTACGCTGCACACCTCATCGGCGGCAAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCGGAAGAAAAGGAAATGATTCGCGCGATGCTGTCGGAATCGCTGCAAGCAGTCATCTCGCAGAC
GCTGCTGAAAACCAAGGACGGCAGCGGTCGCGTCGCCGCGCACGAGATCATGCTGGGCACGCCCGCGATCCGCAACCTGA
TCCGCGAAGCCAAAGTGGCGCAGATGTATTCCACGATTCAGACCGGCAGCAACATCGGCATGCAGACGCTGGACCAGAAT
CTGACCGACCTGGTACGGCGCAATGTGATTTCGCCGGCCGCCGCACGCGCTGCCGCCAAGATTCCGGAAAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

74.336

97.695

0.726
  pilT Legionella pneumophila strain ERS1305867

74.336

97.695

0.726
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.394

95.101

0.66
  pilT Vibrio cholerae strain A1552

69.394

95.101

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

99.424

0.53
  pilU Vibrio cholerae strain A1552

42.183

97.695

0.412
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.593

93.372

0.398

Multiple sequence alignment