Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFGDV_RS23290 Genome accession   NZ_CP176528
Coordinates   5017007..5018143 (+) Length   378 a.a.
NCBI ID   WP_136418843.1    Uniprot ID   -
Organism   Noviherbaspirillum sp. ST 5-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5012007..5023143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFGDV_RS23255 ubiA 5012371..5013225 (+) 855 WP_136418850.1 4-hydroxybenzoate octaprenyltransferase -
  ACFGDV_RS23260 - 5013318..5013632 (+) 315 WP_136418849.1 YqjD family protein -
  ACFGDV_RS23265 - 5013637..5014002 (+) 366 WP_136418848.1 phage holin family protein -
  ACFGDV_RS23270 - 5013999..5014298 (+) 300 WP_136418847.1 YqjK family protein -
  ACFGDV_RS23275 proC 5014307..5015131 (-) 825 WP_136418846.1 pyrroline-5-carboxylate reductase -
  ACFGDV_RS23280 - 5015121..5015825 (-) 705 WP_136418845.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFGDV_RS23285 pilT 5015905..5016948 (+) 1044 WP_136418844.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFGDV_RS23290 pilU 5017007..5018143 (+) 1137 WP_136418843.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFGDV_RS23295 - 5018140..5018625 (+) 486 WP_136418842.1 glutathione peroxidase -
  ACFGDV_RS23300 glcF 5018902..5020134 (-) 1233 WP_136418841.1 glycolate oxidase subunit GlcF -
  ACFGDV_RS23305 glcE 5020137..5021204 (-) 1068 WP_136418840.1 glycolate oxidase subunit GlcE -
  ACFGDV_RS23310 - 5021213..5022700 (-) 1488 WP_136418839.1 FAD-linked oxidase C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41984.48 Da        Isoelectric Point: 6.7691

>NTDB_id=1080729 ACFGDV_RS23290 WP_136418843.1 5017007..5018143(+) (pilU) [Noviherbaspirillum sp. ST 5-3]
MERDQATKFMHDLLRLMLSKNGSDLFITADFPPAFKIDGKMTPVSNQPLTPTHTVELARAIMNDKQAAEFEASKECNFAI
SPGGLGRFRVSAFVQQGRVGMVLRTITTTIPKLEDLGVPEVLKEVAMTKRGLVIMVGATGSGKSTTLAGMVGYRNENSYG
HIITIEDPVEYVHPHKNCIVTQREVGVDTADWFTALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGLISQRLVPLKDIKGRCAAVEIMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEAGKISYEDALRNADSVNDLRLTIKLQGKDAKGRDLSKGTEHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1080729 ACFGDV_RS23290 WP_136418843.1 5017007..5018143(+) (pilU) [Noviherbaspirillum sp. ST 5-3]
ATGGAACGGGACCAGGCCACGAAATTCATGCACGATCTGCTGCGGCTGATGCTCAGCAAGAACGGCTCCGACCTTTTCAT
CACCGCCGATTTCCCGCCGGCATTCAAGATCGACGGCAAGATGACACCGGTCTCGAACCAGCCGCTCACGCCCACGCATA
CCGTCGAACTGGCGCGCGCCATCATGAACGACAAGCAGGCTGCGGAATTCGAAGCGAGCAAGGAGTGCAATTTCGCGATC
AGTCCCGGCGGCCTCGGGCGCTTCCGCGTGTCGGCATTCGTGCAGCAGGGCCGTGTCGGCATGGTGCTGCGGACCATCAC
CACCACGATTCCGAAACTGGAAGACCTGGGCGTGCCGGAGGTGCTGAAGGAAGTGGCGATGACCAAGCGCGGGCTGGTGA
TCATGGTGGGCGCGACCGGCTCGGGCAAATCCACCACGTTGGCCGGCATGGTTGGTTATCGCAATGAAAACAGCTACGGT
CACATCATCACGATTGAAGACCCGGTCGAATACGTGCACCCGCACAAGAACTGCATCGTCACGCAACGCGAAGTCGGCGT
CGATACGGCCGACTGGTTCACCGCACTGAAGAACACACTGCGCCAGGCGCCCGACGTGATTCTGATCGGCGAGATCCGCG
ACCGCGAGACCATGGATTACGCGATCGCGTTTGCCGAAACCGGCCACCTCTGTCTTGCCACGCTGCACGCCAACAGCGCG
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCGGAAGAACGCCGTCAGCAACTCCTGATGGATTTGTCGCTCAACCT
GAAGGGATTGATTTCACAACGTCTCGTCCCCTTGAAAGATATCAAGGGGCGCTGCGCCGCAGTCGAGATAATGTTGAACT
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGCATGAAATCAAGGAAATCATGAAGAAGTCGCGCGAGCTCGGC
ATGCAGACTTTCGACCAGGCACTGTTCGACCTCTACGAGGCCGGCAAGATTTCCTACGAAGACGCGCTGCGCAATGCCGA
CTCGGTCAATGACCTGCGCCTGACCATCAAGCTGCAGGGCAAGGACGCGAAGGGCCGCGACCTGAGCAAGGGAACCGAAC
ATCTGGGAATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.463

96.032

0.619
  pilU Acinetobacter baylyi ADP1

59.331

94.974

0.563
  pilU Vibrio cholerae strain A1552

56.734

92.328

0.524
  pilT Pseudomonas aeruginosa PAK

43.582

88.624

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Pseudomonas stutzeri DSM 10701

42.262

88.889

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.296

87.566

0.37
  pilT Vibrio cholerae strain A1552

42.296

87.566

0.37
  pilT Legionella pneumophila strain ERS1305867

41.994

87.566

0.368
  pilT Legionella pneumophila strain Lp02

41.994

87.566

0.368
  pilT Acinetobacter baylyi ADP1

40.95

89.153

0.365
  pilT Acinetobacter baumannii D1279779

41.692

87.566

0.365
  pilT Acinetobacter baumannii strain A118

41.692

87.566

0.365
  pilT Acinetobacter nosocomialis M2

41.692

87.566

0.365

Multiple sequence alignment