Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACJ33_RS03730 Genome accession   NZ_AP029014
Coordinates   783022..784065 (+) Length   347 a.a.
NCBI ID   WP_075463464.1    Uniprot ID   A0A1U9VFF9
Organism   Ralstonia syzygii subsp. indonesiensis strain MAFF 211271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 778022..789065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACJ33_RS03700 (MAFF211271_07160) - 779177..779419 (+) 243 WP_013211540.1 hypothetical protein -
  AACJ33_RS03705 (MAFF211271_07170) - 779581..780081 (+) 501 WP_003271865.1 Dps family protein -
  AACJ33_RS03710 (MAFF211271_07180) ubiA 780155..781030 (+) 876 WP_118882650.1 4-hydroxybenzoate octaprenyltransferase -
  AACJ33_RS03715 (MAFF211271_07190) - 781027..781305 (-) 279 WP_013211542.1 hypothetical protein -
  AACJ33_RS03720 (MAFF211271_07200) proC 781318..782142 (-) 825 WP_075463462.1 pyrroline-5-carboxylate reductase -
  AACJ33_RS03725 (MAFF211271_07210) - 782177..782899 (-) 723 WP_075463463.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACJ33_RS03730 (MAFF211271_07220) pilT 783022..784065 (+) 1044 WP_075463464.1 type IV pilus twitching motility protein PilT Machinery gene
  AACJ33_RS03735 (MAFF211271_07230) pilU 784118..785254 (+) 1137 WP_013211546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AACJ33_RS03740 (MAFF211271_07240) - 785449..785889 (+) 441 WP_118882651.1 type IV pilin protein -
  AACJ33_RS03745 (MAFF211271_07250) - 785893..786381 (+) 489 WP_075463466.1 GspH/FimT family pseudopilin -
  AACJ33_RS03750 (MAFF211271_07260) pilV 786378..786968 (+) 591 WP_118869211.1 type IV pilus modification protein PilV -
  AACJ33_RS03755 (MAFF211271_07270) - 786965..787987 (+) 1023 WP_075463468.1 PilW family protein -
  AACJ33_RS03760 (MAFF211271_07280) - 787991..788527 (+) 537 WP_075463469.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38124.76 Da        Isoelectric Point: 6.8705

>NTDB_id=108061 AACJ33_RS03730 WP_075463464.1 783022..784065(+) (pilT) [Ralstonia syzygii subsp. indonesiensis strain MAFF 211271]
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPALTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=108061 AACJ33_RS03730 WP_075463464.1 783022..784065(+) (pilT) [Ralstonia syzygii subsp. indonesiensis strain MAFF 211271]
ATGGACATCGCGCAGCTTCTGGCCTTCGCCGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCCCCGAT
GATCCGGATCCATGGCGACATGCGCCGTATCAACGTGCCAGCGCTCACGCACCAGGATGTTCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCCGACTTCTCGTTCGAGATCCCCGGGCTGTCCCGC
TTCCGGGTCAATGCCTTCAACCAGAACCGCGGCGCCGCCGCCGTGTTCCGGACGATTCCGTCCAAGGTCCTGACGCTGGA
AGACCTGAAGGCGCCGGCCGTCTTCGCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAATCACCGCAACGAAAGCGACCTGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAACTGGGGCCACATACCCATTCGTTTGCCAA
CGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCCTGGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCAGCCGAAACCGGCCACTTGGTGTTTGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGATGAGAAGGACATGGTCCGCACCATGTTGTCCGAATCGCTGGAGGCGGTGATTTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGCCACTTGA
TCCGCGAGAACAAGATCTCGCAGATGTATTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGGCGGAGCTCATCAAGCGTTCGGCGATCAACTACGCCGACGCGCGCGCCATCGCCAAGAATCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U9VFF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

71.884

99.424

0.715
  pilT Acinetobacter baumannii strain A118

71.884

99.424

0.715
  pilT Acinetobacter nosocomialis M2

71.884

99.424

0.715
  pilT Pseudomonas stutzeri DSM 10701

71.304

99.424

0.709
  pilT Pseudomonas aeruginosa PAK

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

70.145

99.424

0.697
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

67.536

99.424

0.671
  pilT Legionella pneumophila strain ERS1305867

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.118

0.646
  pilT Vibrio cholerae strain A1552

66.469

97.118

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.578

99.712

0.504
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406

Multiple sequence alignment