Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACJ33_RS03735 Genome accession   NZ_AP029014
Coordinates   784118..785254 (+) Length   378 a.a.
NCBI ID   WP_013211546.1    Uniprot ID   A0AAD0S6R2
Organism   Ralstonia syzygii subsp. indonesiensis strain MAFF 211271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 779118..790254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACJ33_RS03700 (MAFF211271_07160) - 779177..779419 (+) 243 WP_013211540.1 hypothetical protein -
  AACJ33_RS03705 (MAFF211271_07170) - 779581..780081 (+) 501 WP_003271865.1 Dps family protein -
  AACJ33_RS03710 (MAFF211271_07180) ubiA 780155..781030 (+) 876 WP_118882650.1 4-hydroxybenzoate octaprenyltransferase -
  AACJ33_RS03715 (MAFF211271_07190) - 781027..781305 (-) 279 WP_013211542.1 hypothetical protein -
  AACJ33_RS03720 (MAFF211271_07200) proC 781318..782142 (-) 825 WP_075463462.1 pyrroline-5-carboxylate reductase -
  AACJ33_RS03725 (MAFF211271_07210) - 782177..782899 (-) 723 WP_075463463.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACJ33_RS03730 (MAFF211271_07220) pilT 783022..784065 (+) 1044 WP_075463464.1 type IV pilus twitching motility protein PilT Machinery gene
  AACJ33_RS03735 (MAFF211271_07230) pilU 784118..785254 (+) 1137 WP_013211546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AACJ33_RS03740 (MAFF211271_07240) - 785449..785889 (+) 441 WP_118882651.1 type IV pilin protein -
  AACJ33_RS03745 (MAFF211271_07250) - 785893..786381 (+) 489 WP_075463466.1 GspH/FimT family pseudopilin -
  AACJ33_RS03750 (MAFF211271_07260) pilV 786378..786968 (+) 591 WP_118869211.1 type IV pilus modification protein PilV -
  AACJ33_RS03755 (MAFF211271_07270) - 786965..787987 (+) 1023 WP_075463468.1 PilW family protein -
  AACJ33_RS03760 (MAFF211271_07280) - 787991..788527 (+) 537 WP_075463469.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42053.53 Da        Isoelectric Point: 7.0670

>NTDB_id=108062 AACJ33_RS03735 WP_013211546.1 784118..785254(+) (pilU) [Ralstonia syzygii subsp. indonesiensis strain MAFF 211271]
MLDREAATKYIHELLQLMVNSRGSDLFITSEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMNEKQLREFEGSFECNFA
IVSGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSYG
HIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANSS
NQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGRKGRAPAIEILLGTPLVADLIFKGEVHELKEVMKKSREQG
MVSFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=108062 AACJ33_RS03735 WP_013211546.1 784118..785254(+) (pilU) [Ralstonia syzygii subsp. indonesiensis strain MAFF 211271]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGATTTGTT
CATCACCTCGGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGTTGACCACCGTCC
AGGCGATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAGTTGCGCGAGTTCGAGGGCTCCTTCGAGTGCAACTTTGCC
ATCGTGTCCGGCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCATCAA
CACCAAGATCCCGACGCTCGGCGAACTGGATCTGCCGCCCATCCTCAACGAGGTCGCGATGAGCAAGCGCGGGCTCGTGG
TCGTGGTGGGGGCGACGGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTACGGC
CACATCATCACCATTGAGGATCCGGTGGAATACGTGCACGCACACCAGAACTGCGTCGTGACGCAGCGCGAGGTGGGCGT
GGATACGGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ATCGCGACACGATGGAGTACGCCATCCAGTACGCCGAAACCGGCCACTTGTGCCTGGCTACGCTGCACGCCAACAGCTCG
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGCTGCTGATCGACCTGTCCTTGAACCT
GCGCGCCATGATTGCGCAGCGCCTGCTGCCGCGCAAAGGCAGGAAGGGCCGCGCGCCGGCGATCGAAATCCTGCTGGGCA
CGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGGTGCATGAGCTCAAGGAGGTCATGAAGAAGTCCCGCGAGCAGGGG
ATGGTCTCGTTCGACCAGGCGCTGTTCGAGCTCTATGAGGCGGACAAGATCACCTATGAGGACGCGCTGCGCAACGCAGA
CTCGCTGAACGATCTGCGTCTGCAGATCAAGCTGCACAGCAAGCGCGGCGGGCCGGTGGACCTGACGGCCGGCACCGAGC
ATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.606

93.386

0.585
  pilU Vibrio cholerae strain A1552

56.091

93.386

0.524
  pilU Acinetobacter baylyi ADP1

54.596

94.974

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas aeruginosa PAK

42.73

89.153

0.381
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Vibrio cholerae strain A1552

44.795

83.862

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.795

83.862

0.376
  pilT Acinetobacter baylyi ADP1

44.099

85.185

0.376
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376
  pilT Acinetobacter baumannii D1279779

43.26

84.392

0.365
  pilT Acinetobacter baumannii strain A118

43.26

84.392

0.365
  pilT Acinetobacter nosocomialis M2

43.26

84.392

0.365

Multiple sequence alignment