Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJ3Y0_RS10945 Genome accession   NZ_CP176471
Coordinates   2376447..2377556 (+) Length   369 a.a.
NCBI ID   WP_372383818.1    Uniprot ID   -
Organism   Vibrio sp. BS-M-Sm-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2371447..2382556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3Y0_RS10910 (ACJ3Y0_10910) - 2371719..2372318 (-) 600 WP_146490584.1 XTP/dITP diphosphatase -
  ACJ3Y0_RS10915 (ACJ3Y0_10915) - 2372367..2372798 (-) 432 WP_372383816.1 DUF4426 domain-containing protein -
  ACJ3Y0_RS10920 (ACJ3Y0_10920) yggU 2372827..2373117 (-) 291 WP_372383817.1 DUF167 family protein YggU -
  ACJ3Y0_RS10925 (ACJ3Y0_10925) - 2373147..2373704 (-) 558 WP_017067798.1 YggT family protein -
  ACJ3Y0_RS10930 (ACJ3Y0_10930) proC 2373763..2374581 (-) 819 WP_146490587.1 pyrroline-5-carboxylate reductase -
  ACJ3Y0_RS10935 (ACJ3Y0_10935) - 2374664..2375368 (-) 705 WP_146490588.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJ3Y0_RS10940 (ACJ3Y0_10940) pilT 2375395..2376432 (+) 1038 WP_060982714.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ3Y0_RS10945 (ACJ3Y0_10945) pilU 2376447..2377556 (+) 1110 WP_372383818.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJ3Y0_RS10950 (ACJ3Y0_10950) - 2377627..2377785 (-) 159 WP_186728038.1 hypothetical protein -
  ACJ3Y0_RS10955 (ACJ3Y0_10955) ruvX 2377884..2378306 (-) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  ACJ3Y0_RS10960 (ACJ3Y0_10960) - 2378355..2378918 (-) 564 WP_048611435.1 YqgE/AlgH family protein -
  ACJ3Y0_RS10965 (ACJ3Y0_10965) gshB 2379035..2379985 (-) 951 WP_061018824.1 glutathione synthase -
  ACJ3Y0_RS10970 (ACJ3Y0_10970) rsmE 2379995..2380726 (-) 732 WP_060982711.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACJ3Y0_RS10975 (ACJ3Y0_10975) - 2380880..2381611 (-) 732 WP_050644132.1 endonuclease -
  ACJ3Y0_RS10980 (ACJ3Y0_10980) - 2381763..2382263 (-) 501 WP_285954962.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41397.23 Da        Isoelectric Point: 5.6697

>NTDB_id=1080252 ACJ3Y0_RS10945 WP_372383818.1 2376447..2377556(+) (pilU) [Vibrio sp. BS-M-Sm-2]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGDKLNSAQVGQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYETGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1080252 ACJ3Y0_RS10945 WP_372383818.1 2376447..2377556(+) (pilU) [Vibrio sp. BS-M-Sm-2]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTGTCTCAAAAAGCATCGGATCTTTATATCACCGTTGATGCGCCTATCCT
TTTTCGTGTTGATGGTGAATTGCGACCTCAAGGAGATAAGCTGAATTCGGCTCAGGTCGGACAGTTGCTTGACGCGATGA
TGGATCAAGATCGACGCGATGAATATCAGCAAACGCGTGAGGCTAACTTTGCTATTGTGCGTGATTTTGGTCGTTTTCGT
GTCAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTCATTCGACGCATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTACTACAGGACCTTTCAATTGCTAAGCGCGGACTTGTGCTGGTGGTTGGGGCGACTGGCTCTGGTA
AATCGACCTCAATGGCTGCAATGACAGGCTATCGTAACACCAATCGTTCCGGTCATATATTGACGGTTGAAGACCCTATT
GAATTTGTCCACGAACATAAAAAGTGCATCGTGACGCAACGTGAGGTTGGACTTGACACTGAGAGCTATGAAGTTGCGCT
TAAGAACTCGTTACGCCAAGCACCAGATATGATTTTGATTGGTGAAATTCGTAGCCGTGAGACCATGGAATACGCGATGA
CTTTTGCTGAGACTGGTCACTTGTGTATGGCAACCTTGCACGCCAATAACGCTAACCAAGCGTTAGAGCGTATTCTCCAC
TTGGTGCCGAAAGAGCAGAAAGAACAGTTTTTGTTTGATCTGTCGATGAACCTACGTGGTGTCGTCGCTCAGCAACTCAT
CCGTGATAAGAATGGCAGCGGGCGTCATGGTGTGTTTGAGATTTTACTTAACAGCCCCCGAGTGTCTGACTTGATTCGTC
GCGGTGAACTGCACGAGCTAAAAGCAACAATGGCGAAATCGAAAGAGATTGGTATGCAGACCTTTGATCAAGCTTTGTAT
GATTTAGTTGTAGCAGGTAAGATCAGCGAAGAGGATGCGTTTCACAGTGCTGACTCTGCTAATGACTTACGTTTGATGCT
AAAAACCAGACGTGGAGATGATGACTACGAGACTGGTGCTTTGGCCGGGGTAAAGATAGACATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

99.729

0.84
  pilU Pseudomonas stutzeri DSM 10701

56.604

100

0.569
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Acinetobacter baumannii strain A118

42.353

92.141

0.39
  pilT Acinetobacter baumannii D1279779

42.353

92.141

0.39
  pilT Acinetobacter nosocomialis M2

42.353

92.141

0.39
  pilT Acinetobacter baylyi ADP1

41.813

92.683

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.593

87.805

0.374
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363

Multiple sequence alignment