Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJ3Y0_RS10940 Genome accession   NZ_CP176471
Coordinates   2375395..2376432 (+) Length   345 a.a.
NCBI ID   WP_060982714.1    Uniprot ID   -
Organism   Vibrio sp. BS-M-Sm-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2370395..2381432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3Y0_RS10905 (ACJ3Y0_10905) hemW 2370532..2371719 (-) 1188 WP_372383815.1 radical SAM family heme chaperone HemW -
  ACJ3Y0_RS10910 (ACJ3Y0_10910) - 2371719..2372318 (-) 600 WP_146490584.1 XTP/dITP diphosphatase -
  ACJ3Y0_RS10915 (ACJ3Y0_10915) - 2372367..2372798 (-) 432 WP_372383816.1 DUF4426 domain-containing protein -
  ACJ3Y0_RS10920 (ACJ3Y0_10920) yggU 2372827..2373117 (-) 291 WP_372383817.1 DUF167 family protein YggU -
  ACJ3Y0_RS10925 (ACJ3Y0_10925) - 2373147..2373704 (-) 558 WP_017067798.1 YggT family protein -
  ACJ3Y0_RS10930 (ACJ3Y0_10930) proC 2373763..2374581 (-) 819 WP_146490587.1 pyrroline-5-carboxylate reductase -
  ACJ3Y0_RS10935 (ACJ3Y0_10935) - 2374664..2375368 (-) 705 WP_146490588.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJ3Y0_RS10940 (ACJ3Y0_10940) pilT 2375395..2376432 (+) 1038 WP_060982714.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ3Y0_RS10945 (ACJ3Y0_10945) pilU 2376447..2377556 (+) 1110 WP_372383818.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJ3Y0_RS10950 (ACJ3Y0_10950) - 2377627..2377785 (-) 159 WP_186728038.1 hypothetical protein -
  ACJ3Y0_RS10955 (ACJ3Y0_10955) ruvX 2377884..2378306 (-) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  ACJ3Y0_RS10960 (ACJ3Y0_10960) - 2378355..2378918 (-) 564 WP_048611435.1 YqgE/AlgH family protein -
  ACJ3Y0_RS10965 (ACJ3Y0_10965) gshB 2379035..2379985 (-) 951 WP_061018824.1 glutathione synthase -
  ACJ3Y0_RS10970 (ACJ3Y0_10970) rsmE 2379995..2380726 (-) 732 WP_060982711.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38271.80 Da        Isoelectric Point: 6.0507

>NTDB_id=1080251 ACJ3Y0_RS10940 WP_060982714.1 2375395..2376432(+) (pilT) [Vibrio sp. BS-M-Sm-2]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLDQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVERKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1080251 ACJ3Y0_RS10940 WP_060982714.1 2375395..2376432(+) (pilT) [Vibrio sp. BS-M-Sm-2]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGCGATTCACAACGCGGTGAGTTTGAAGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGCCGT
TTCCGTGTGAATGCTTTTAACCAAGCACGTGGTTGCTCAGCTGTCTTTCGAACAATCCCTGTAGAGATCCCAACTTTAGA
TCAGTTAGGTGCACCTGAGATCTTTGAAAAGATTGCTAACTACGAAAAAGGTTTAGTGCTTGTCACTGGCCCTACTGGTT
CTGGTAAATCGACGACTCTCGCAGCGATGGTTGATTACGTAAACCGCAACCACAATAAACACATCCTGACGATCGAAGAC
CCAATTGAATTCGTTCATACCAACAATAAATGCCTTGTTAACCAGCGTGAAGTTCATCGTGATACTCACAGCTTTAAAGC
AGCGCTGCGCAGCGCGTTACGTGAAGACCCAGACGTTATTCTTGTGGGTGAGCTTCGTGACCAAGAGACCATTAGCTTAG
CGCTAACCGCAGCAGAAACTGGTCACTTGGTTTTTGGTACTTTGCATACCAGCTCCGCGGCTAAAACTATAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGCTGTCCGAGTCGTTGCGCTCTGTGATTGCTCAGAA
GCTGCTAAAGCGTGTTGGTGGCGGTCGTATCGCGTGTCATGAAATCATGATGGCGACACCTGCGATCCGAAACCTGATTC
GTGAAGACAAGGTTGCTCAGATGTATTCGATCATTCAAACGGGTGCAGCACATGGTATGCAAACCATGGAGCAAAATGCG
AAGCAGCTAATGGCTCAAGGCTTGGTTGATTCGGAAGAGGTCGAGAGAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

71.687

96.232

0.69
  pilT Acinetobacter baumannii strain A118

71.687

96.232

0.69
  pilT Acinetobacter nosocomialis M2

71.386

96.232

0.687
  pilT Acinetobacter baylyi ADP1

70.606

95.652

0.675
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.217

100

0.652
  pilT Legionella pneumophila strain ERS1305867

65.217

100

0.652
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.812

0.394
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment