Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJ3W8_RS13185 Genome accession   NZ_CP176466
Coordinates   2749709..2750749 (-) Length   346 a.a.
NCBI ID   WP_409423903.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. RW-H-Ap-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2744709..2755749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3W8_RS13155 (ACJ3W8_13155) - 2744924..2745397 (+) 474 WP_409423897.1 hypothetical protein -
  ACJ3W8_RS13160 (ACJ3W8_13160) rsmE 2745468..2746202 (+) 735 WP_409423898.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACJ3W8_RS13165 (ACJ3W8_13165) gshB 2746257..2747207 (+) 951 WP_409423899.1 glutathione synthase -
  ACJ3W8_RS13170 (ACJ3W8_13170) - 2747335..2747892 (+) 558 WP_409423900.1 YqgE/AlgH family protein -
  ACJ3W8_RS13175 (ACJ3W8_13175) ruvX 2747910..2748359 (+) 450 WP_409423901.1 Holliday junction resolvase RuvX -
  ACJ3W8_RS13180 (ACJ3W8_13180) pilU 2748563..2749699 (-) 1137 WP_409423902.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJ3W8_RS13185 (ACJ3W8_13185) pilT 2749709..2750749 (-) 1041 WP_409423903.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ3W8_RS13190 (ACJ3W8_13190) - 2750752..2751465 (+) 714 WP_409423904.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJ3W8_RS13195 (ACJ3W8_13195) proC 2751483..2752304 (+) 822 WP_409423905.1 pyrroline-5-carboxylate reductase -
  ACJ3W8_RS13200 (ACJ3W8_13200) - 2752331..2752864 (+) 534 WP_409423906.1 YggT family protein -
  ACJ3W8_RS13205 (ACJ3W8_13205) - 2752922..2753365 (+) 444 WP_058549460.1 DUF4426 domain-containing protein -
  ACJ3W8_RS13210 (ACJ3W8_13210) - 2753370..2753969 (+) 600 WP_303441585.1 XTP/dITP diphosphatase -
  ACJ3W8_RS13215 (ACJ3W8_13215) hemW 2753966..2755102 (+) 1137 WP_409423907.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38304.55 Da        Isoelectric Point: 6.3160

>NTDB_id=1080170 ACJ3W8_RS13185 WP_409423903.1 2749709..2750749(-) (pilT) [Pseudoalteromonas sp. RW-H-Ap-1]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALADKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLEDLGAPEIFKQISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQNKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMIRSMLSESLRAVISQTLIKKVGGGRVAAHEIMLATPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
TNLVNRGIITHSAAYTKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1080170 ACJ3W8_RS13185 WP_409423903.1 2749709..2750749(-) (pilT) [Pseudoalteromonas sp. RW-H-Ap-1]
ATGGATATTACCGAATTATTGGCGTTTAGTGTGCAACACAAAGCATCCGATTTACACTTATCGTCGGGTGTTTCACCTAT
GATACGCGTAGATGGTGATGTTCGCCGCATTAATATACCTGCACTTGCAGACAAAGACGTAAACAGTCTGGTTTACGACA
TTATGAACGATAATCAACGCAAGGACTACGAACAAAATCTTGAAGTGGATTTTTCGTTTGAAGTACCTAACCTTGCTCGC
TTTCGTGTGAATGCATTTAACTCTAATCGCGGACCTGCAGCTGTGTTTCGTACTATACCAAGTGAGGTACTCACACTCGA
AGATTTAGGCGCGCCGGAAATATTTAAACAAATTTCAGATAACCCACGAGGCTTAGTCTTAGTTACCGGTCCTACAGGCT
CAGGTAAGTCGACTACACTCGCAGCCATGGTGGACTATATAAATCAAAATAAACATCACCATATTCTGACTATAGAAGAT
CCTATCGAATTTGTTCACGACAATAAACTAAGCCTTATTAACCAACGTGAAGTTCACCGCGATACGCACAGCTTTTCAAA
TGCACTGCGCAGTGCATTGCGTGAAGATCCCGATGTAATATTGGTTGGTGAGCTTCGTGATTTAGAAACTATTCGTCTTG
CTATGACTGCGGCTGAAACCGGTCACTTAGTATTTGGTACTTTGCATACCACGTCGGCACCTAAAACCATTGACCGTATT
ATTGATGTATTCCCAGGTGAAGAAAAAGACATGATCCGCTCTATGCTTTCCGAATCATTGCGTGCGGTTATCTCACAAAC
ACTGATCAAGAAGGTCGGTGGTGGGCGAGTGGCTGCACACGAAATTATGTTGGCAACGCCCGCTATTCGTAACTTAATCC
GTGAAGATAAAATTGCGCAAATGTATTCATCAATTCAAACAGGTGCATCGCACGGTATGCAAACAATGGATCAATGTTTA
ACAAATTTAGTTAACCGAGGCATTATCACTCACTCTGCGGCGTATACAAAAGCGCAAGATAAAACACAGTTTGGCGGTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.035

99.422

0.766
  pilT Acinetobacter baumannii D1279779

77.035

99.422

0.766
  pilT Acinetobacter baumannii strain A118

77.035

99.422

0.766
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.422

0.76
  pilT Pseudomonas aeruginosa PAK

75.872

99.422

0.754
  pilT Acinetobacter baylyi ADP1

75.581

99.422

0.751
  pilT Legionella pneumophila strain Lp02

72.674

99.422

0.723
  pilT Legionella pneumophila strain ERS1305867

72.674

99.422

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.164

95.087

0.705
  pilT Vibrio cholerae strain A1552

74.164

95.087

0.705
  pilT Neisseria gonorrhoeae MS11

70.088

98.555

0.691
  pilT Neisseria meningitidis 8013

70.088

98.555

0.691
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

99.711

0.526
  pilU Vibrio cholerae strain A1552

42.769

93.931

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.528

97.977

0.387
  pilU Acinetobacter baylyi ADP1

39.118

98.266

0.384

Multiple sequence alignment