Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACKUBV_RS14070 Genome accession   NZ_CP176357
Coordinates   2961372..2962478 (+) Length   368 a.a.
NCBI ID   WP_021454419.1    Uniprot ID   A0A7H5D0D6
Organism   Vibrio parahaemolyticus strain AG1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2956372..2967478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUBV_RS14035 (ACKUBV_14035) - 2956442..2957044 (-) 603 WP_323208611.1 XTP/dITP diphosphatase -
  ACKUBV_RS14040 (ACKUBV_14040) - 2957162..2957593 (-) 432 WP_005461703.1 DUF4426 domain-containing protein -
  ACKUBV_RS14045 (ACKUBV_14045) yggU 2957721..2958011 (-) 291 WP_005482461.1 DUF167 family protein YggU -
  ACKUBV_RS14050 (ACKUBV_14050) - 2958011..2958568 (-) 558 WP_005482440.1 YggT family protein -
  ACKUBV_RS14055 (ACKUBV_14055) proC 2958621..2959439 (-) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  ACKUBV_RS14060 (ACKUBV_14060) - 2959559..2960272 (-) 714 WP_069536405.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACKUBV_RS14065 (ACKUBV_14065) pilT 2960301..2961341 (+) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKUBV_RS14070 (ACKUBV_14070) pilU 2961372..2962478 (+) 1107 WP_021454419.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACKUBV_RS14075 (ACKUBV_14075) ruvX 2962540..2962965 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  ACKUBV_RS14080 (ACKUBV_14080) - 2963014..2963577 (-) 564 WP_005461717.1 YqgE/AlgH family protein -
  ACKUBV_RS14085 (ACKUBV_14085) gshB 2963691..2964641 (-) 951 WP_005449741.1 glutathione synthase -
  ACKUBV_RS14090 (ACKUBV_14090) rsmE 2964656..2965387 (-) 732 WP_017449132.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACKUBV_RS14095 (ACKUBV_14095) endA 2965512..2966207 (-) 696 WP_005493878.1 deoxyribonuclease I -
  ACKUBV_RS14100 (ACKUBV_14100) - 2966327..2966824 (-) 498 WP_020835372.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41429.68 Da        Isoelectric Point: 7.0235

>NTDB_id=1079725 ACKUBV_RS14070 WP_021454419.1 2961372..2962478(+) (pilU) [Vibrio parahaemolyticus strain AG1]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
ISAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1079725 ACKUBV_RS14070 WP_021454419.1 2961372..2962478(+) (pilU) [Vibrio parahaemolyticus strain AG1]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAACATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGATCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
ATTAGTGCTTTTTTTCAGCGAGAACTGCCGGGAGCAGTCATTCGCCGTATCGAAACCATTATTCCTACTTTTGAGCAGTT
AAAATTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCTACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAGGTGGGCTTGGATACCGAAAGTTATGAAGTCGCACT
GAAAAACTCGTTGCGCCAAGCGCCTGATATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAACGTATTCTCCAT
TTGGTGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTGATAAGAATGGTCAAGGGCGTCATGGTGTGTTTGAGATCTTGCTAAACAGTCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACACGAATTGAAGTCAACCATGGCACGATCCAATGAGTTTGGTATGCTGACGTTTGACCAATCGCTCTAC
AAGCTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCATTGCACAGTGCGGATTCAGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H5D0D6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.923

88.315

0.397
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361

Multiple sequence alignment