Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACKUBV_RS14065 Genome accession   NZ_CP176357
Coordinates   2960301..2961341 (+) Length   346 a.a.
NCBI ID   WP_005461712.1    Uniprot ID   Q87LJ7
Organism   Vibrio parahaemolyticus strain AG1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2955301..2966341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUBV_RS14035 (ACKUBV_14035) - 2956442..2957044 (-) 603 WP_323208611.1 XTP/dITP diphosphatase -
  ACKUBV_RS14040 (ACKUBV_14040) - 2957162..2957593 (-) 432 WP_005461703.1 DUF4426 domain-containing protein -
  ACKUBV_RS14045 (ACKUBV_14045) yggU 2957721..2958011 (-) 291 WP_005482461.1 DUF167 family protein YggU -
  ACKUBV_RS14050 (ACKUBV_14050) - 2958011..2958568 (-) 558 WP_005482440.1 YggT family protein -
  ACKUBV_RS14055 (ACKUBV_14055) proC 2958621..2959439 (-) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  ACKUBV_RS14060 (ACKUBV_14060) - 2959559..2960272 (-) 714 WP_069536405.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACKUBV_RS14065 (ACKUBV_14065) pilT 2960301..2961341 (+) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKUBV_RS14070 (ACKUBV_14070) pilU 2961372..2962478 (+) 1107 WP_021454419.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACKUBV_RS14075 (ACKUBV_14075) ruvX 2962540..2962965 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  ACKUBV_RS14080 (ACKUBV_14080) - 2963014..2963577 (-) 564 WP_005461717.1 YqgE/AlgH family protein -
  ACKUBV_RS14085 (ACKUBV_14085) gshB 2963691..2964641 (-) 951 WP_005449741.1 glutathione synthase -
  ACKUBV_RS14090 (ACKUBV_14090) rsmE 2964656..2965387 (-) 732 WP_017449132.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACKUBV_RS14095 (ACKUBV_14095) endA 2965512..2966207 (-) 696 WP_005493878.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38562.05 Da        Isoelectric Point: 6.5039

>NTDB_id=1079724 ACKUBV_RS14065 WP_005461712.1 2960301..2961341(+) (pilT) [Vibrio parahaemolyticus strain AG1]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1079724 ACKUBV_RS14065 WP_005461712.1 2960301..2961341(+) (pilT) [Vibrio parahaemolyticus strain AG1]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCCCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTGTTCCGTACCATCCCAAGTAGCATCCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATCGCGAATGCTGAAAAAGGTTTGGTTTTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACAACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCATATCCTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAGTGTTTGATCAACCAACGTGAAGTTCACCGTGATACCCATAGCTTCCAAAA
CGCGCTACGCAGCGCATTGCGTGAAGACCCGGATGTGATCCTTGTCGGTGAGATGCGTGACAAAGAAACCATCAGCCTAG
CGTTAACGGCGGCGGAAACGGGTCACTTAGTTTTCGGTACGTTGCACACCAGTTCAGCAGCAAAAACCATCGACCGTATC
ATCGATGTGTTCCCAGGTAGTGACAAAGACATGGTGCGTTCCATGTTGTCTGAATCCCTTCGTTCAGTTATCGCGCAAAA
ACTGTTGAAACGTAATGGCGGTGGTCGTATCGCTTGTCACGAAATAATGATGGCAACGCCCGCGATTCGTAACTTGATTC
GCGAAGACAAAGTCGCGCAGATGTACTCAATCATTCAAACGGGTGCCGCGCATGGAATGCAAACCATGGAACAAAATGCC
CGTCAGTTGATGGCGCAAGGCATGGTTTCGCGCGAAGAAGTCGACAGCAAGATTGGGCTTGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

99.711

0.87
  pilT Vibrio cholerae strain A1552

87.246

99.711

0.87
  pilT Acinetobacter baumannii D1279779

72.727

95.376

0.694
  pilT Acinetobacter baumannii strain A118

72.727

95.376

0.694
  pilT Acinetobacter nosocomialis M2

72.424

95.376

0.691
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

69.733

97.399

0.679
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.399

0.676
  pilT Legionella pneumophila strain Lp02

68.278

95.665

0.653
  pilT Legionella pneumophila strain ERS1305867

68.278

95.665

0.653
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.145

91.908

0.488
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.532

0.396
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364

Multiple sequence alignment