Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJU26_RS14415 Genome accession   NZ_CP175559
Coordinates   2767229..2768278 (-) Length   349 a.a.
NCBI ID   WP_414040288.1    Uniprot ID   -
Organism   Acidithiobacillus sp. M4-SHS-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2762229..2773278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJU26_RS14390 (ACJU26_14390) purE 2762480..2762977 (-) 498 WP_414040283.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  ACJU26_RS14395 (ACJU26_14395) trmB 2763028..2763732 (+) 705 WP_414040284.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACJU26_RS14400 (ACJU26_14400) - 2763746..2764873 (+) 1128 WP_414040285.1 NAD(P)/FAD-dependent oxidoreductase -
  ACJU26_RS14405 (ACJU26_14405) - 2764889..2766094 (-) 1206 WP_414040286.1 glycosyltransferase -
  ACJU26_RS14410 (ACJU26_14410) pilU 2766094..2767218 (-) 1125 WP_414040287.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJU26_RS14415 (ACJU26_14415) pilT 2767229..2768278 (-) 1050 WP_414040288.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJU26_RS14420 (ACJU26_14420) - 2768381..2769052 (+) 672 WP_414040289.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJU26_RS14425 (ACJU26_14425) proC 2769049..2769876 (+) 828 WP_414040290.1 pyrroline-5-carboxylate reductase -
  ACJU26_RS14430 (ACJU26_14430) - 2769873..2770160 (+) 288 WP_414040291.1 YggT family protein -
  ACJU26_RS14435 (ACJU26_14435) bioB 2770197..2771141 (+) 945 WP_414040292.1 biotin synthase BioB -
  ACJU26_RS14440 (ACJU26_14440) - 2771148..2771711 (-) 564 WP_414040293.1 acyloxyacyl hydrolase -
  ACJU26_RS14445 (ACJU26_14445) - 2771949..2772962 (+) 1014 WP_414040294.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38548.16 Da        Isoelectric Point: 6.5786

>NTDB_id=1078203 ACJU26_RS14415 WP_414040288.1 2767229..2768278(-) (pilT) [Acidithiobacillus sp. M4-SHS-6]
MDIAELLTFAVKNNASDTHISAGLAPMLRINGDMRPLNVEALDSKAVHAMIYDIMSDSQRKWYEENLEIDFAYELPGIAR
FRINAFNQDRGPAAAFRTIPANVLSLEALNAPKSFTEIINIPRGLVLVTGPTGSGKSTTLAAMVDHINSNRPDHIITIED
PIEFIHTAKKSLVNQREVGPHTHSFENALRSALREDPDIILVGELRDLETMRLALTAAETGHIVFATLHTSSAPKTIDRI
VDSFPGGEKDMVRAMLSESLRAVISQTLLKTADGKGRVPAHEIMIATPAIRNLIRENKVAQMYSVIQTGQNQGMQTLDQC
LADLVRAHKITREEALRRVQNKDSFMNVA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=1078203 ACJU26_RS14415 WP_414040288.1 2767229..2768278(-) (pilT) [Acidithiobacillus sp. M4-SHS-6]
ATGGATATCGCTGAATTGCTCACCTTCGCGGTGAAAAATAATGCTTCGGATACGCATATTTCAGCAGGGCTCGCCCCTAT
GTTGCGCATCAACGGAGATATGCGCCCTCTCAACGTGGAGGCGCTGGATAGCAAGGCCGTCCACGCGATGATTTATGACA
TCATGTCAGACTCCCAGCGCAAATGGTATGAAGAAAATCTGGAAATCGATTTTGCCTATGAGTTACCGGGCATTGCCCGC
TTTCGGATAAATGCCTTTAACCAGGACCGGGGACCAGCGGCGGCATTTCGGACCATTCCCGCCAATGTGCTGAGTCTGGA
AGCGTTGAATGCCCCGAAATCCTTTACAGAAATTATTAACATACCCCGTGGGCTCGTGCTGGTGACGGGGCCGACCGGAT
CGGGAAAGTCCACGACATTGGCTGCCATGGTCGATCACATCAACAGCAATCGGCCGGATCACATCATTACCATCGAAGAC
CCCATTGAATTTATCCACACGGCGAAGAAGTCGCTGGTGAACCAGCGTGAGGTCGGGCCCCATACCCATTCCTTCGAAAA
CGCCTTGCGTTCGGCCCTGCGGGAAGATCCGGATATCATTCTGGTGGGTGAATTAAGGGATCTGGAAACCATGCGTCTGG
CCTTGACCGCAGCAGAAACCGGGCACATCGTGTTTGCCACCCTGCATACCAGTTCTGCGCCAAAAACCATTGATCGTATT
GTGGATTCATTTCCAGGGGGAGAAAAGGACATGGTTCGGGCCATGCTCTCGGAATCCTTGCGGGCAGTCATCTCCCAGAC
CCTGCTCAAGACCGCCGATGGAAAAGGCCGGGTACCGGCGCACGAAATCATGATCGCCACTCCCGCCATTCGCAATCTGA
TTCGCGAAAACAAAGTAGCGCAGATGTATTCGGTCATTCAGACCGGACAAAATCAGGGCATGCAAACCCTGGATCAGTGT
CTGGCGGATTTGGTGCGGGCGCACAAAATCACCCGCGAGGAAGCCCTGCGCCGGGTGCAGAACAAAGACAGTTTCATGAA
CGTGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

66.763

99.14

0.662
  pilT Acinetobacter baumannii strain A118

66.763

99.14

0.662
  pilT Acinetobacter baumannii D1279779

66.763

99.14

0.662
  pilT Pseudomonas stutzeri DSM 10701

66.377

98.854

0.656
  pilT Pseudomonas aeruginosa PAK

66.087

98.854

0.653
  pilT Legionella pneumophila strain Lp02

65.217

98.854

0.645
  pilT Neisseria meningitidis 8013

65.217

98.854

0.645
  pilT Legionella pneumophila strain ERS1305867

65.217

98.854

0.645
  pilT Neisseria gonorrhoeae MS11

64.928

98.854

0.642
  pilT Acinetobacter baylyi ADP1

68.615

93.123

0.639
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.412

97.421

0.628
  pilT Vibrio cholerae strain A1552

64.412

97.421

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.857

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

41.667

96.275

0.401
  pilU Acinetobacter baylyi ADP1

41.124

96.848

0.398
  pilU Vibrio cholerae strain A1552

39.881

96.275

0.384

Multiple sequence alignment