Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJU26_RS14410 Genome accession   NZ_CP175559
Coordinates   2766094..2767218 (-) Length   374 a.a.
NCBI ID   WP_414040287.1    Uniprot ID   -
Organism   Acidithiobacillus sp. M4-SHS-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2761094..2772218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJU26_RS14385 (ACJU26_14385) - 2761350..2762483 (-) 1134 WP_414040282.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  ACJU26_RS14390 (ACJU26_14390) purE 2762480..2762977 (-) 498 WP_414040283.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  ACJU26_RS14395 (ACJU26_14395) trmB 2763028..2763732 (+) 705 WP_414040284.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACJU26_RS14400 (ACJU26_14400) - 2763746..2764873 (+) 1128 WP_414040285.1 NAD(P)/FAD-dependent oxidoreductase -
  ACJU26_RS14405 (ACJU26_14405) - 2764889..2766094 (-) 1206 WP_414040286.1 glycosyltransferase -
  ACJU26_RS14410 (ACJU26_14410) pilU 2766094..2767218 (-) 1125 WP_414040287.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJU26_RS14415 (ACJU26_14415) pilT 2767229..2768278 (-) 1050 WP_414040288.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJU26_RS14420 (ACJU26_14420) - 2768381..2769052 (+) 672 WP_414040289.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJU26_RS14425 (ACJU26_14425) proC 2769049..2769876 (+) 828 WP_414040290.1 pyrroline-5-carboxylate reductase -
  ACJU26_RS14430 (ACJU26_14430) - 2769873..2770160 (+) 288 WP_414040291.1 YggT family protein -
  ACJU26_RS14435 (ACJU26_14435) bioB 2770197..2771141 (+) 945 WP_414040292.1 biotin synthase BioB -
  ACJU26_RS14440 (ACJU26_14440) - 2771148..2771711 (-) 564 WP_414040293.1 acyloxyacyl hydrolase -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41463.74 Da        Isoelectric Point: 5.4923

>NTDB_id=1078202 ACJU26_RS14410 WP_414040287.1 2766094..2767218(-) (pilU) [Acidithiobacillus sp. M4-SHS-6]
MSVLNDLLALMVEKNGSDLYLTVGSPPVMKMDGRVVNLGTEMLKPGQVLSLAQEMLGMERLQEFQQEKEINMAISATGIG
RFRVNGFYQRGELSFVLRAIKTQIPTLEQLQLPPILKDLAMTSRGLVLFVGATGSGKSTSLASMIEFRNQNAAGHILTIE
DPIEYLHKNSKSIVNQREVGIDTRNYERALENALREAPDVILIGEIRNRDTMDHAMAYAETGHLCMSTLHANNANQAIER
IINFFPEDRKKQLLMDLSLNLKAVVSQRLLPVKGQAGRIAAVEVLINTPAIADLIYKGEVGLLKEAMGRTNDMGMQTFDQ
SLLRLYADGLVEYEDALRAADSQNDLRLAIKQDCLRRGLDDPGAAHSAETQWQV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=1078202 ACJU26_RS14410 WP_414040287.1 2766094..2767218(-) (pilU) [Acidithiobacillus sp. M4-SHS-6]
ATGTCGGTACTGAATGATCTATTGGCGCTGATGGTCGAAAAAAATGGTTCGGATCTCTATCTGACAGTGGGTTCCCCACC
CGTCATGAAAATGGATGGAAGGGTGGTGAACCTGGGTACGGAGATGCTGAAACCCGGACAGGTACTGAGTCTTGCCCAGG
AAATGCTGGGGATGGAGCGCCTGCAGGAATTCCAGCAGGAAAAAGAAATCAACATGGCGATTTCGGCCACGGGGATCGGG
CGTTTTCGGGTCAATGGCTTTTACCAGCGCGGTGAATTGAGTTTTGTATTGCGTGCCATCAAAACCCAGATCCCGACTTT
GGAACAACTCCAGTTGCCACCCATCCTCAAGGATCTCGCCATGACTTCCCGAGGCTTGGTGCTGTTTGTCGGGGCGACGG
GTTCGGGCAAAAGCACTTCTCTCGCCTCCATGATTGAGTTTCGCAACCAGAACGCCGCCGGGCATATCCTGACCATTGAG
GACCCTATTGAATATCTTCACAAAAACAGCAAATCCATCGTCAATCAGCGCGAGGTGGGTATTGATACCCGCAATTATGA
AAGAGCCCTGGAAAATGCCCTGCGCGAAGCCCCCGATGTGATCCTGATCGGCGAAATACGCAATCGGGATACCATGGATC
ATGCCATGGCCTATGCGGAAACCGGGCACTTGTGCATGTCTACCCTGCATGCCAATAACGCCAACCAGGCCATTGAGCGC
ATCATCAACTTTTTCCCCGAAGACCGGAAAAAACAATTGCTGATGGATTTGTCCCTGAACTTGAAGGCCGTCGTTTCCCA
GCGCTTGCTGCCTGTCAAGGGGCAGGCGGGACGCATTGCGGCTGTGGAAGTGCTGATCAATACCCCGGCCATTGCCGATC
TCATTTACAAAGGCGAGGTGGGACTGCTCAAGGAGGCCATGGGGCGCACCAACGATATGGGCATGCAAACCTTCGATCAA
AGTTTGCTCAGGCTCTATGCCGATGGGCTGGTGGAATATGAAGACGCATTGCGCGCCGCCGATTCCCAGAATGATCTGCG
TTTGGCCATCAAACAGGATTGCCTGCGCCGGGGGCTGGACGACCCGGGCGCTGCCCATTCTGCCGAAACCCAGTGGCAGG
TCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.87

92.246

0.562
  pilU Vibrio cholerae strain A1552

53.186

96.524

0.513
  pilU Acinetobacter baylyi ADP1

53.868

93.316

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.651

92.781

0.396
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.771

88.77

0.38
  pilT Vibrio cholerae strain A1552

42.771

88.77

0.38
  pilT Acinetobacter baylyi ADP1

40.708

90.642

0.369
  pilT Legionella pneumophila strain Lp02

40.896

89.572

0.366
  pilT Legionella pneumophila strain ERS1305867

40.896

89.572

0.366
  pilT Acinetobacter baumannii strain A118

40.653

90.107

0.366
  pilT Acinetobacter baumannii D1279779

40.653

90.107

0.366
  pilT Acinetobacter nosocomialis M2

40.356

90.107

0.364
  pilT Pseudomonas aeruginosa PAK

40.356

90.107

0.364

Multiple sequence alignment