Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJZU6_RS03945 Genome accession   NZ_CP175534
Coordinates   803604..804641 (+) Length   345 a.a.
NCBI ID   WP_080158259.1    Uniprot ID   A0A1T5I2M0
Organism   Photobacterium piscicola strain WVL24019     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 798604..809641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJZU6_RS03910 (ACJZU6_03910) - 798655..799053 (+) 399 WP_158258949.1 DUF2884 family protein -
  ACJZU6_RS03915 (ACJZU6_03915) hemW 799170..800330 (-) 1161 WP_408900675.1 radical SAM family heme chaperone HemW -
  ACJZU6_RS03920 (ACJZU6_03920) - 800334..800924 (-) 591 WP_080158263.1 XTP/dITP diphosphatase -
  ACJZU6_RS03925 (ACJZU6_03925) yggU 801097..801393 (-) 297 WP_045037795.1 DUF167 family protein YggU -
  ACJZU6_RS03930 (ACJZU6_03930) - 801390..801947 (-) 558 WP_080158262.1 YggT family protein -
  ACJZU6_RS03935 (ACJZU6_03935) proC 801957..802775 (-) 819 WP_080158261.1 pyrroline-5-carboxylate reductase -
  ACJZU6_RS03940 (ACJZU6_03940) - 802868..803578 (-) 711 WP_408900676.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJZU6_RS03945 (ACJZU6_03945) pilT 803604..804641 (+) 1038 WP_080158259.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJZU6_RS03950 (ACJZU6_03950) pilU 804663..805766 (+) 1104 WP_408900677.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJZU6_RS03955 (ACJZU6_03955) ruvX 806125..806556 (-) 432 WP_036793273.1 Holliday junction resolvase RuvX -
  ACJZU6_RS03960 (ACJZU6_03960) - 806553..807116 (-) 564 WP_080158257.1 YqgE/AlgH family protein -
  ACJZU6_RS03965 (ACJZU6_03965) gshB 807237..808187 (-) 951 WP_080158256.1 glutathione synthase -
  ACJZU6_RS03970 (ACJZU6_03970) rsmE 808205..808936 (-) 732 WP_408900678.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACJZU6_RS03975 (ACJZU6_03975) - 809069..809554 (-) 486 WP_408900915.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38333.94 Da        Isoelectric Point: 6.4392

>NTDB_id=1077919 ACJZU6_RS03945 WP_080158259.1 803604..804641(+) (pilT) [Photobacterium piscicola strain WVL24019]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALTHSEVHRLIFDIMNDAQRREYEEKLEVDFSFELPDIGR
FRINAFHQSRGCSAVLRTIPIHIPTLASLNVPEVFYDIAQLNRGLVLVTGATGSGKSTTIAALIDYINSNYQRHILTIED
PIEFVHTSKSCLINQREVHRDTLSFQNALRSSLREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGHDKSMVRSMLSESLRAVVAQNLLKCTSGGRVACHEVMMATPAIRNLIREDKIAQMYSMIQTGSSLGMQTMEQSV
KMLVAQGMVEAEEGRRVLDRSKSNF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1077919 ACJZU6_RS03945 WP_080158259.1 803604..804641(+) (pilT) [Photobacterium piscicola strain WVL24019]
ATGGATATCACCGAATTACTCGATTTTAGTGTAAAGCATAACGCATCAGATCTGCATCTTTCTGCGGGTGTTCCACCAAT
GATAAGAGTTGATGGCGATGTAAGAAAGCTCAGTTTGCCTGCTTTAACTCACAGTGAAGTGCATCGATTGATTTTTGATA
TTATGAACGATGCCCAACGTCGAGAGTATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAATTACCTGATATCGGCCGT
TTTAGGATTAACGCTTTTCATCAATCGCGCGGTTGTTCTGCTGTGCTGCGTACCATCCCTATTCATATTCCTACTTTAGC
ATCATTGAATGTGCCTGAGGTTTTTTATGATATTGCCCAGCTTAACCGTGGTCTCGTGTTAGTGACGGGCGCAACGGGCT
CGGGTAAATCAACCACTATTGCCGCCTTAATAGATTACATCAATAGCAACTATCAACGCCATATTTTGACGATTGAAGAT
CCGATTGAATTTGTCCATACCAGTAAAAGTTGTTTGATCAACCAACGTGAAGTGCATCGTGACACGCTGAGCTTTCAAAA
TGCCCTGCGGTCATCGTTACGTGAAGATCCCGATGTGATTGTGGTGGGTGAGTTGCGCGATCAAGAAACGATTAGTTTGG
CATTAACGGCGGCTGAAACCGGACATTTAGTTTTAGGCACCTTGCATACTAGCTCAGCGGCTAAAACGGTCGATCGTATT
ATTGACGTCTTTCCCGGCCATGATAAATCGATGGTACGTTCAATGTTATCTGAATCATTACGCGCGGTTGTGGCTCAAAA
TTTATTGAAGTGTACTTCTGGTGGGCGAGTTGCTTGTCATGAAGTGATGATGGCTACCCCCGCTATTCGAAATTTAATCC
GTGAAGATAAAATTGCTCAGATGTATTCAATGATTCAAACCGGTTCTTCACTTGGCATGCAGACTATGGAGCAAAGTGTA
AAAATGCTGGTGGCACAAGGAATGGTCGAAGCTGAAGAAGGACGTCGTGTTCTGGATCGTAGCAAAAGCAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T5I2M0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

78.378

96.522

0.757
  pilT Vibrio cholerae strain A1552

78.378

96.522

0.757
  pilT Acinetobacter baylyi ADP1

67.638

99.42

0.672
  pilT Acinetobacter baumannii strain A118

67.347

99.42

0.67
  pilT Acinetobacter baumannii D1279779

67.347

99.42

0.67
  pilT Acinetobacter nosocomialis M2

67.055

99.42

0.667
  pilT Pseudomonas stutzeri DSM 10701

68.06

97.101

0.661
  pilT Pseudomonas aeruginosa PAK

68.06

97.101

0.661
  pilT Legionella pneumophila strain Lp02

68.693

95.362

0.655
  pilT Legionella pneumophila strain ERS1305867

68.693

95.362

0.655
  pilT Neisseria meningitidis 8013

63.081

99.71

0.629
  pilT Neisseria gonorrhoeae MS11

62.791

99.71

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.56

98.841

0.49
  pilU Pseudomonas stutzeri DSM 10701

42.216

96.812

0.409
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Acinetobacter baylyi ADP1

39.441

93.333

0.368

Multiple sequence alignment