Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJZU6_RS03950 Genome accession   NZ_CP175534
Coordinates   804663..805766 (+) Length   367 a.a.
NCBI ID   WP_408900677.1    Uniprot ID   -
Organism   Photobacterium piscicola strain WVL24019     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 799663..810766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJZU6_RS03920 (ACJZU6_03920) - 800334..800924 (-) 591 WP_080158263.1 XTP/dITP diphosphatase -
  ACJZU6_RS03925 (ACJZU6_03925) yggU 801097..801393 (-) 297 WP_045037795.1 DUF167 family protein YggU -
  ACJZU6_RS03930 (ACJZU6_03930) - 801390..801947 (-) 558 WP_080158262.1 YggT family protein -
  ACJZU6_RS03935 (ACJZU6_03935) proC 801957..802775 (-) 819 WP_080158261.1 pyrroline-5-carboxylate reductase -
  ACJZU6_RS03940 (ACJZU6_03940) - 802868..803578 (-) 711 WP_408900676.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJZU6_RS03945 (ACJZU6_03945) pilT 803604..804641 (+) 1038 WP_080158259.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJZU6_RS03950 (ACJZU6_03950) pilU 804663..805766 (+) 1104 WP_408900677.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJZU6_RS03955 (ACJZU6_03955) ruvX 806125..806556 (-) 432 WP_036793273.1 Holliday junction resolvase RuvX -
  ACJZU6_RS03960 (ACJZU6_03960) - 806553..807116 (-) 564 WP_080158257.1 YqgE/AlgH family protein -
  ACJZU6_RS03965 (ACJZU6_03965) gshB 807237..808187 (-) 951 WP_080158256.1 glutathione synthase -
  ACJZU6_RS03970 (ACJZU6_03970) rsmE 808205..808936 (-) 732 WP_408900678.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACJZU6_RS03975 (ACJZU6_03975) - 809069..809554 (-) 486 WP_408900915.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40915.83 Da        Isoelectric Point: 5.9670

>NTDB_id=1077920 ACJZU6_RS03950 WP_408900677.1 804663..805766(+) (pilU) [Photobacterium piscicola strain WVL24019]
MTLDDILEQMVAKKASDLYITVASACLLKIDGNLHSVGEVLNRSGVDSLFDQAMDTNLRQEFFSTKEANFALVRNDLRFR
VSAFWQQELPGMVIRRIETQIPQMSDLKLPIDMERLALSKRGLVLIVGATGSGKSTSMAAMTGYRNQHRSGHILTVEDPI
EFVHKHNKCIVTQREVGLDTASYEVALKNSLRQAPDMILIGEIRTSETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCILAQQLIADANGEGRHGVYELLLNTPRVADLIRRGELYEIKGVMAKSTEAGMRTFDQSLY
ALFNEGKISEQDALHHADSANDLRLMIKVGDPDANSLNSLSGVALEN

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=1077920 ACJZU6_RS03950 WP_408900677.1 804663..805766(+) (pilU) [Photobacterium piscicola strain WVL24019]
ATGACGTTAGATGACATTCTTGAGCAAATGGTAGCTAAAAAAGCTTCTGATTTATACATCACTGTTGCTTCAGCTTGCTT
ATTAAAAATTGATGGTAATTTACATAGTGTCGGTGAAGTGTTAAATCGAAGTGGTGTTGATAGCCTATTTGATCAAGCTA
TGGATACGAACCTTAGACAAGAATTTTTTAGCACCAAAGAAGCTAATTTTGCTTTAGTTCGTAATGACCTTCGATTTCGT
GTCAGTGCTTTTTGGCAACAAGAATTACCTGGAATGGTGATTCGTCGTATTGAAACCCAAATACCACAAATGAGTGATTT
GAAATTACCCATAGATATGGAACGACTGGCACTTTCAAAGCGAGGTTTAGTTCTTATTGTTGGTGCTACGGGATCGGGAA
AATCAACCTCGATGGCGGCAATGACAGGTTATCGAAATCAGCATCGAAGTGGTCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTTCACAAGCACAACAAATGCATTGTGACTCAGCGCGAAGTTGGACTGGATACTGCCAGTTATGAAGTGGCGCT
AAAAAACTCCTTACGCCAAGCGCCAGACATGATTTTAATCGGTGAAATTCGTACTAGTGAAACCATGGAATACGCGATGA
ATTTTGCTGAGACGGGCCATTTATGTATGGCAACTTTGCATGCCAATAATGCTAACCAAGCATTAGAACGTATTTTACAT
TTAGTGCCTAAAGAGCGACGTGATCAATTTTTATTTGATTTATCACTTAATTTAAAATGTATTTTGGCGCAGCAATTAAT
CGCAGATGCTAATGGAGAGGGGCGTCATGGTGTGTATGAGTTATTACTTAATACACCACGAGTGGCAGATTTAATTCGAC
GCGGTGAGTTGTATGAAATTAAAGGTGTAATGGCCAAATCGACTGAAGCTGGAATGAGAACGTTTGATCAATCATTGTAT
GCATTATTTAATGAAGGAAAGATCAGCGAGCAAGATGCACTTCATCACGCCGACTCTGCTAACGATTTACGGTTGATGAT
CAAAGTGGGTGATCCTGATGCTAATTCATTGAATAGCTTATCGGGTGTGGCATTAGAAAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(67-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

69.399

99.728

0.692
  pilU Pseudomonas stutzeri DSM 10701

57.219

100

0.583
  pilU Acinetobacter baylyi ADP1

55.085

96.458

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.356

91.826

0.371

Multiple sequence alignment