Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OAG1_RS04470 Genome accession   NZ_AP028990
Coordinates   960396..961430 (+) Length   344 a.a.
NCBI ID   WP_016401371.1    Uniprot ID   R9PK19
Organism   Agarivorans sp. OAG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 955396..966430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OAG1_RS04440 (OAG1_08690) rdgB 956354..956953 (-) 600 WP_338624326.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OAG1_RS04445 (OAG1_08700) - 957316..957750 (-) 435 WP_338624328.1 DUF4426 domain-containing protein -
  OAG1_RS04450 (OAG1_08710) yggU 957809..958099 (-) 291 WP_338624330.1 DUF167 family protein YggU -
  OAG1_RS04455 (OAG1_08720) - 958100..958645 (-) 546 WP_016401374.1 YggT family protein -
  OAG1_RS04460 (OAG1_08730) proC 958663..959484 (-) 822 WP_338624332.1 pyrroline-5-carboxylate reductase -
  OAG1_RS04465 (OAG1_08740) - 959672..960370 (-) 699 WP_338624334.1 YggS family pyridoxal phosphate-dependent enzyme -
  OAG1_RS04470 (OAG1_08750) pilT 960396..961430 (+) 1035 WP_016401371.1 type IV pilus twitching motility protein PilT Machinery gene
  OAG1_RS04475 (OAG1_08760) pilU 961439..962557 (+) 1119 WP_338624336.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OAG1_RS04480 (OAG1_08770) yaaA 962575..963354 (+) 780 WP_338624338.1 peroxide stress protein YaaA -
  OAG1_RS04485 (OAG1_08780) - 963510..963977 (+) 468 WP_338624340.1 hypothetical protein -
  OAG1_RS04490 (OAG1_08790) - 964186..964929 (+) 744 WP_016401367.1 OmpA family protein -
  OAG1_RS04495 (OAG1_08800) srmB 964992..966248 (-) 1257 WP_016401366.1 ATP-dependent RNA helicase SrmB -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38335.92 Da        Isoelectric Point: 6.3901

>NTDB_id=107765 OAG1_RS04470 WP_016401371.1 960396..961430(+) (pilT) [Agarivorans sp. OAG1]
MDVTELLAFSVKHNASDLHLSAELPPMIRVDGEVRKINVPALEHKQVHSLIYDIMNDKQRKEYEENLEIDFSFEVPGVAR
FRVNAFQQSRGAAAVFRTIPSEVLSLEQLGAPAIFRQIAEHPRGLVLVTGPTGSGKSTTLAAMIDYINEQRHEHILTIED
PIEFVHKNKNCLINQREVHRDTHSFNNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLRAVIAQTLLKKLGGGRVAAHEIMTGIPAIRNLIREDKVAQMYSVIQTGMAHGMQTLDQNL
KELVNQGMVSYEDAKLKAADPNNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=107765 OAG1_RS04470 WP_016401371.1 960396..961430(+) (pilT) [Agarivorans sp. OAG1]
ATGGATGTTACTGAATTACTGGCCTTTAGTGTAAAACATAACGCGTCGGATCTACACCTATCTGCAGAATTGCCACCGAT
GATTCGGGTAGACGGCGAAGTACGTAAAATTAACGTACCGGCATTAGAGCATAAACAAGTTCATAGTCTGATCTACGACA
TTATGAACGATAAGCAACGTAAAGAGTATGAAGAAAACCTCGAGATTGACTTCTCGTTTGAGGTTCCCGGCGTAGCGCGC
TTTCGTGTGAATGCCTTCCAACAAAGCCGCGGCGCGGCAGCGGTATTTCGTACCATTCCTTCAGAAGTATTGAGTTTAGA
GCAACTAGGTGCACCGGCCATTTTTAGGCAGATCGCGGAACACCCACGTGGTTTAGTGCTAGTAACTGGCCCAACTGGTT
CTGGTAAATCAACCACATTGGCGGCAATGATCGATTACATTAATGAGCAGCGCCACGAGCACATCCTTACCATTGAAGAC
CCGATAGAATTTGTGCACAAAAACAAAAACTGTTTGATTAACCAACGTGAAGTGCACCGCGATACTCATAGCTTTAACAA
TGCGCTACGTTCAGCGCTTCGTGAAGACCCGGATATTATTCTGGTTGGTGAATTACGTGACTTAGAAACCATTCGTCTAG
CACTCACCGCGGCCGAAACGGGTCACTTAGTATTTGGCACCTTGCACACCACCTCAGCAGCTAAAACCATTGACCGTGTG
ATTGACGTATTCCCAGCAGCAGAAAAAGACATGGTGCGCTCCATGTTGTCGGAATCGCTGCGTGCGGTAATTGCTCAAAC
TCTGCTTAAAAAACTGGGCGGCGGACGAGTGGCTGCTCACGAAATCATGACCGGTATTCCAGCTATTCGTAACTTGATTC
GTGAAGACAAAGTGGCGCAAATGTACTCGGTTATTCAAACCGGTATGGCGCACGGCATGCAGACCTTGGATCAAAACTTA
AAAGAGTTAGTGAACCAAGGCATGGTGTCGTATGAAGACGCTAAACTTAAAGCTGCAGATCCGAATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R9PK19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Pseudomonas stutzeri DSM 10701

78.488

100

0.785
  pilT Pseudomonas aeruginosa PAK

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

75

100

0.75
  pilT Legionella pneumophila strain ERS1305867

75

100

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.965

0.735
  pilT Vibrio cholerae strain A1552

75.074

97.965

0.735
  pilT Neisseria meningitidis 8013

68.328

99.128

0.677
  pilT Neisseria gonorrhoeae MS11

68.035

99.128

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.506
  pilU Vibrio cholerae strain A1552

41.57

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404

Multiple sequence alignment