Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJJVG_RS18840 Genome accession   NZ_CP174503
Coordinates   4050275..4051255 (-) Length   326 a.a.
NCBI ID   WP_407731591.1    Uniprot ID   -
Organism   Pseudocitrobacter faecalis strain AHC.AZy     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4045275..4056255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJVG_RS18810 (ACJJVG_18810) endA 4045380..4046087 (+) 708 WP_407731588.1 deoxyribonuclease I -
  ACJJVG_RS18815 (ACJJVG_18815) rsmE 4046210..4046941 (+) 732 WP_407731589.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACJJVG_RS18820 (ACJJVG_18820) gshB 4046965..4047912 (+) 948 WP_407731590.1 glutathione synthase -
  ACJJVG_RS18825 (ACJJVG_18825) - 4048057..4048620 (+) 564 WP_108475813.1 YqgE/AlgH family protein -
  ACJJVG_RS18830 (ACJJVG_18830) ruvX 4048620..4049042 (+) 423 WP_108475812.1 Holliday junction resolvase RuvX -
  ACJJVG_RS18835 (ACJJVG_18835) - 4049137..4049790 (-) 654 WP_015963067.1 LuxR C-terminal-related transcriptional regulator -
  ACJJVG_RS18840 (ACJJVG_18840) pilT 4050275..4051255 (-) 981 WP_407731591.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJJVG_RS18845 (ACJJVG_18845) - 4051273..4051986 (+) 714 WP_108475810.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJJVG_RS18850 (ACJJVG_18850) - 4051995..4052561 (+) 567 WP_108475809.1 YggT family protein -
  ACJJVG_RS18855 (ACJJVG_18855) yggU 4052558..4052848 (+) 291 WP_407731592.1 DUF167 family protein YggU -
  ACJJVG_RS18860 (ACJJVG_18860) - 4052857..4053450 (+) 594 WP_108475807.1 XTP/dITP diphosphatase -
  ACJJVG_RS18865 (ACJJVG_18865) hemW 4053443..4054579 (+) 1137 WP_407731593.1 radical SAM family heme chaperone HemW -
  ACJJVG_RS18870 (ACJJVG_18870) ansB 4054618..4055664 (-) 1047 WP_407731594.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35344.61 Da        Isoelectric Point: 6.5567

>NTDB_id=1077434 ACJJVG_RS18840 WP_407731591.1 4050275..4051255(-) (pilT) [Pseudocitrobacter faecalis strain AHC.AZy]
MEIQEIVALSVKHNVSDLHLCSGAMARWRRCGMLESAPFSSPEPEELLRVWLSEAQHAQWRAQGQLDFAIVPSEVTRLRA
SAFRHARGVSLALRVLPSCCPTLAELGVPAALPELLHEEGGLILVTGATGSGKSTTLAAMVDHLNHSLDGHILTLEDPVE
FIHASQRCLIQQREIGLHISSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDV
FPAQEKEPVRNQLANSLCAVVAQKLVADKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVMQTGQQAGMQTFTQSYQQC
MARGNL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1077434 ACJJVG_RS18840 WP_407731591.1 4050275..4051255(-) (pilT) [Pseudocitrobacter faecalis strain AHC.AZy]
ATGGAAATACAAGAAATCGTGGCGCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGTAGCGGGGCAATGGCGCG
ATGGCGGCGTTGCGGAATGCTGGAGTCTGCGCCATTTTCATCGCCAGAGCCGGAAGAACTGCTGCGTGTTTGGTTAAGTG
AAGCACAGCATGCGCAATGGCGAGCGCAGGGCCAGCTTGATTTTGCGATAGTGCCATCTGAAGTAACACGCCTGCGCGCC
AGCGCATTTCGCCATGCGCGCGGCGTTTCGCTGGCATTGCGTGTGCTACCGTCGTGCTGCCCAACACTCGCTGAACTCGG
CGTGCCTGCGGCCCTCCCTGAACTGTTACACGAAGAGGGCGGGCTAATATTGGTCACTGGCGCGACCGGCAGCGGGAAGT
CAACGACCCTCGCCGCGATGGTCGACCATCTGAATCACTCGCTGGATGGGCATATTCTGACGCTGGAGGATCCGGTGGAG
TTTATCCATGCCAGTCAGCGCTGCTTAATTCAACAACGCGAAATTGGCCTGCATATCTCTTCATTTGCTGATGCACTGCG
TGGCGCGTTACGTCAGGACCCCGATGTTATTTTGCTGGGAGAATTACGCGATAGCGAGACGATCCGGCTGGCATTAACGG
CTGCTGAAACAGGCCATCTGGTGCTGGCAACATTGCACACGCGTGGGGCAGCCCAGGCGATTGAGCGTCTGGTGGACGTT
TTCCCGGCGCAGGAAAAAGAGCCTGTACGCAATCAATTAGCGAACAGTTTATGTGCCGTCGTCGCGCAAAAACTGGTGGC
GGATAAGCAAGGCGGGCGCGTGGCGCTCTTCGAACTGCTGGTGAACACCCCGGCGGTGGGAAATCTGATCCGTGAAGGCA
AAACCCATCAGCTACCGGGTGTCATGCAAACTGGACAGCAGGCAGGGATGCAAACTTTTACGCAGAGCTATCAACAATGT
ATGGCGAGGGGTAATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

100

0.5
  pilT Vibrio cholerae strain A1552

49.394

100

0.5
  pilT Neisseria meningitidis 8013

48.338

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.036

100

0.488
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.872

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.11

97.239

0.439
  pilU Vibrio cholerae strain A1552

41.049

99.387

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Acinetobacter baylyi ADP1

37.461

99.08

0.371
  pilB Haemophilus influenzae 86-028NP

35.417

100

0.365

Multiple sequence alignment