Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI703_RS15610 Genome accession   NZ_CP173765
Coordinates   3471440..3472477 (-) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Isoptericola jiangsuensis strain JSH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3466440..3477477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI703_RS15585 (ACI703_15585) - 3467023..3468006 (-) 984 WP_313206693.1 aldo/keto reductase -
  ACI703_RS15590 (ACI703_15590) - 3468098..3468676 (-) 579 WP_200751423.1 hypothetical protein -
  ACI703_RS15595 (ACI703_15595) - 3468754..3469335 (-) 582 WP_406232687.1 RDD family protein -
  ACI703_RS15600 (ACI703_15600) - 3469445..3470146 (-) 702 WP_406232689.1 hypothetical protein -
  ACI703_RS15605 (ACI703_15605) pilU 3470192..3471313 (-) 1122 WP_420883920.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI703_RS15610 (ACI703_15610) pilT 3471440..3472477 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI703_RS15615 (ACI703_15615) - 3472565..3473242 (+) 678 WP_406232695.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI703_RS15620 (ACI703_15620) proC 3473258..3474079 (+) 822 WP_164075569.1 pyrroline-5-carboxylate reductase -
  ACI703_RS15625 (ACI703_15625) - 3474511..3475383 (-) 873 WP_200751417.1 LysR family transcriptional regulator -
  ACI703_RS15630 (ACI703_15630) - 3475480..3476082 (+) 603 WP_406232698.1 short chain dehydrogenase -
  ACI703_RS15635 (ACI703_15635) soxR 3476151..3476585 (-) 435 WP_108768011.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=1073984 ACI703_RS15610 WP_004147099.1 3471440..3472477(-) (pilT) [Isoptericola jiangsuensis strain JSH01]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1073984 ACI703_RS15610 WP_004147099.1 3471440..3472477(-) (pilT) [Isoptericola jiangsuensis strain JSH01]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCTGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGATGTTCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCCTTCGAGATTCCGTCGCTGGCGCGC
TTCCGTGTCAACGCCTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTTGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
AGCACTGCGCTCGGCACTGCGTGAGGATCCGGACATCATCCTGGTGGGCGAACTCCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTCATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTCGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGAATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCTTGATCACCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGTCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment