Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACI703_RS15605 Genome accession   NZ_CP173765
Coordinates   3470192..3471313 (-) Length   373 a.a.
NCBI ID   WP_420883920.1    Uniprot ID   -
Organism   Isoptericola jiangsuensis strain JSH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3465192..3476313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI703_RS15575 (ACI703_15575) - 3465522..3465734 (-) 213 WP_108768022.1 DUF4287 domain-containing protein -
  ACI703_RS15580 (ACI703_15580) - 3466055..3466951 (+) 897 WP_406232685.1 LysR substrate-binding domain-containing protein -
  ACI703_RS15585 (ACI703_15585) - 3467023..3468006 (-) 984 WP_313206693.1 aldo/keto reductase -
  ACI703_RS15590 (ACI703_15590) - 3468098..3468676 (-) 579 WP_200751423.1 hypothetical protein -
  ACI703_RS15595 (ACI703_15595) - 3468754..3469335 (-) 582 WP_406232687.1 RDD family protein -
  ACI703_RS15600 (ACI703_15600) - 3469445..3470146 (-) 702 WP_406232689.1 hypothetical protein -
  ACI703_RS15605 (ACI703_15605) pilU 3470192..3471313 (-) 1122 WP_420883920.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI703_RS15610 (ACI703_15610) pilT 3471440..3472477 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI703_RS15615 (ACI703_15615) - 3472565..3473242 (+) 678 WP_406232695.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI703_RS15620 (ACI703_15620) proC 3473258..3474079 (+) 822 WP_164075569.1 pyrroline-5-carboxylate reductase -
  ACI703_RS15625 (ACI703_15625) - 3474511..3475383 (-) 873 WP_200751417.1 LysR family transcriptional regulator -
  ACI703_RS15630 (ACI703_15630) - 3475480..3476082 (+) 603 WP_406232698.1 short chain dehydrogenase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41647.93 Da        Isoelectric Point: 6.5902

>NTDB_id=1073983 ACI703_RS15605 WP_420883920.1 3470192..3471313(-) (pilU) [Isoptericola jiangsuensis strain JSH01]
MDFTSFLKLMAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLAGVGR
FRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIED
PIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRI
INFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKELMKESVELGMKTFDQS
LFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1073983 ACI703_RS15605 WP_420883920.1 3470192..3471313(-) (pilU) [Isoptericola jiangsuensis strain JSH01]
ATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGATCTGTTCATCACCGCGGGCATGCCGCCGGC
GATCAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACCTGGTCCTCAACG
TGATGACCCCGGCACAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCCTGGCCGGTGTCGGTCGT
TTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGTATCGAGACGCGCATCCCGACCGTGGA
AGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTCTTCGTCGGTGCCACCGGTA
CCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGAC
CCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGC
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCATGGACCACG
CCATCGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATC
ATCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGATCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCA
GCTCGTTCCTTCGCCCGATGGCCGTTCGCGCAAGGTCGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACA
TCCGCGACGGCGAGATCCACAAGCTCAAGGAGCTGATGAAGGAATCGGTCGAGTTGGGCATGAAGACCTTCGACCAGAGC
CTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGATTCGCAGAACGAAGTGCGCCT
GCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCCTGTCGCAGGGCCTGGATGGCGTGGAGATTTCCGAAGTTCGTT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.392

98.391

0.673
  pilU Acinetobacter baylyi ADP1

64.706

95.71

0.619
  pilU Vibrio cholerae strain A1552

53.933

95.442

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.254

95.174

0.402
  pilT Acinetobacter baumannii strain A118

40.407

92.225

0.373
  pilT Acinetobacter nosocomialis M2

40.407

92.225

0.373
  pilT Acinetobacter baumannii D1279779

40.407

92.225

0.373
  pilT Legionella pneumophila strain Lp02

40.896

89.812

0.367
  pilT Legionella pneumophila strain ERS1305867

40.896

89.812

0.367
  pilT Pseudomonas aeruginosa PAK

40.597

89.812

0.365

Multiple sequence alignment