Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACI703_RS04550 Genome accession   NZ_CP173765
Coordinates   1026120..1026530 (+) Length   136 a.a.
NCBI ID   WP_406234967.1    Uniprot ID   -
Organism   Isoptericola jiangsuensis strain JSH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1021120..1031530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI703_RS04525 (ACI703_04525) - 1021295..1021711 (+) 417 WP_406234959.1 hypothetical protein -
  ACI703_RS04530 (ACI703_04530) - 1021853..1022863 (-) 1011 WP_406234961.1 AI-2E family transporter -
  ACI703_RS04535 (ACI703_04535) coaE 1023010..1023621 (-) 612 WP_406234963.1 dephospho-CoA kinase -
  ACI703_RS04540 (ACI703_04540) - 1023632..1024495 (-) 864 WP_406234965.1 prepilin peptidase -
  ACI703_RS04545 (ACI703_04545) pilC 1024504..1025763 (-) 1260 WP_313207607.1 type II secretion system F family protein Machinery gene
  ACI703_RS04550 (ACI703_04550) pilA2 1026120..1026530 (+) 411 WP_406234967.1 pilin Machinery gene
  ACI703_RS04555 (ACI703_04555) - 1026618..1028579 (+) 1962 WP_406234969.1 hypothetical protein -
  ACI703_RS04560 (ACI703_04560) - 1028572..1029018 (+) 447 WP_406234971.1 GtrA family protein -
  ACI703_RS04565 (ACI703_04565) - 1029015..1030559 (+) 1545 WP_406234973.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14146.17 Da        Isoelectric Point: 8.9934

>NTDB_id=1073975 ACI703_RS04550 WP_406234967.1 1026120..1026530(+) (pilA2) [Isoptericola jiangsuensis strain JSH01]
MKNQKGFTLIELMIVVAIIAILAAIALPAYSDYTKKSKVSEVILAASSLRTAVAEYAASNNALPADSWSGYEAQSSKYVK
SVAWAGNKITATAQGIDGAINDKTIELSASLNSTNGTVAWTCGGGIPAKFRPGSCQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1073975 ACI703_RS04550 WP_406234967.1 1026120..1026530(+) (pilA2) [Isoptericola jiangsuensis strain JSH01]
ATGAAGAACCAGAAGGGCTTCACCCTCATCGAACTGATGATCGTCGTCGCGATCATCGCGATCCTGGCTGCCATCGCACT
GCCGGCTTACTCCGACTACACCAAGAAGTCCAAGGTCTCGGAAGTGATCCTGGCCGCTTCGTCGCTGCGTACCGCAGTGG
CCGAGTACGCTGCTTCCAACAATGCCCTGCCGGCTGATTCGTGGAGCGGCTATGAGGCGCAGTCGTCCAAGTACGTGAAG
AGCGTGGCCTGGGCCGGCAACAAGATCACCGCCACTGCTCAGGGCATTGATGGCGCCATCAATGACAAGACCATCGAACT
GTCCGCTTCGCTGAATTCGACCAACGGCACTGTCGCTTGGACCTGCGGCGGCGGCATTCCGGCCAAGTTCCGTCCGGGCA
GCTGCCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

50

100

0.515
  pilA2 Legionella pneumophila str. Paris

50

100

0.515
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.842

100

0.515
  pilA Acinetobacter baumannii strain A118

41.958

100

0.441
  comP Acinetobacter baylyi ADP1

40.411

100

0.434
  pilE Neisseria gonorrhoeae strain FA1090

46.825

92.647

0.434
  pilE Neisseria gonorrhoeae MS11

44.882

93.382

0.419
  pilA/pilA1 Eikenella corrodens VA1

36.538

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.286

100

0.404
  pilA Haemophilus influenzae 86-028NP

39.855

100

0.404
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.849

100

0.397
  pilA Pseudomonas aeruginosa PAK

36

100

0.397
  pilA Glaesserella parasuis strain SC1401

36.364

100

0.382
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.247

100

0.368
  pilA Vibrio cholerae strain A1552

34.247

100

0.368
  pilA Vibrio cholerae C6706

34.247

100

0.368

Multiple sequence alignment