Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI3MP_RS11720 Genome accession   NZ_CP173446
Coordinates   2450383..2451363 (+) Length   326 a.a.
NCBI ID   WP_001605850.1    Uniprot ID   -
Organism   Escherichia coli strain 98232     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2445383..2456363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3MP_RS11690 (ACI3MP_11690) yggM 2445988..2446995 (+) 1008 WP_001605851.1 DUF1202 family protein -
  ACI3MP_RS11695 (ACI3MP_11695) hemW 2447060..2448196 (-) 1137 WP_000239950.1 radical SAM family heme chaperone HemW -
  ACI3MP_RS11700 (ACI3MP_11700) rdgB 2448189..2448782 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  ACI3MP_RS11705 (ACI3MP_11705) yggU 2448790..2449080 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  ACI3MP_RS11710 (ACI3MP_11710) yggT 2449077..2449643 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  ACI3MP_RS11715 (ACI3MP_11715) yggS 2449661..2450365 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  ACI3MP_RS11720 (ACI3MP_11720) pilT 2450383..2451363 (+) 981 WP_001605850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI3MP_RS11725 (ACI3MP_11725) ruvX 2451538..2451954 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  ACI3MP_RS11730 (ACI3MP_11730) yqgE 2451954..2452517 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  ACI3MP_RS11735 (ACI3MP_11735) gshB 2452626..2453576 (-) 951 WP_001605849.1 glutathione synthase -
  ACI3MP_RS11740 (ACI3MP_11740) rsmE 2453589..2454320 (-) 732 WP_001605848.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACI3MP_RS11745 (ACI3MP_11745) endA 2454400..2455107 (-) 708 WP_000286500.1 deoxyribonuclease I -
  ACI3MP_RS11750 (ACI3MP_11750) yggI 2455202..2455699 (-) 498 WP_096976488.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35894.12 Da        Isoelectric Point: 5.7654

>NTDB_id=1072423 ACI3MP_RS11720 WP_001605850.1 2450383..2451363(+) (pilT) [Escherichia coli strain 98232]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPLILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPARNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1072423 ACI3MP_RS11720 WP_001605850.1 2450383..2451363(+) (pilT) [Escherichia coli strain 98232]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACTGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGCACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment