Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI537_RS00870 Genome accession   NZ_CP173426
Coordinates   185028..186059 (+) Length   343 a.a.
NCBI ID   WP_084209471.1    Uniprot ID   -
Organism   Cobetia marina strain KMM 1449     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 180028..191059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI537_RS00845 (ACI537_00845) metW 180224..180838 (-) 615 WP_077379447.1 methionine biosynthesis protein MetW -
  ACI537_RS00850 (ACI537_00850) - 180831..182024 (-) 1194 WP_077379977.1 homoserine O-acetyltransferase -
  ACI537_RS00855 (ACI537_00855) - 182295..182873 (-) 579 WP_077379444.1 YggT family protein -
  ACI537_RS00860 (ACI537_00860) proC 183043..183885 (-) 843 WP_136939426.1 pyrroline-5-carboxylate reductase -
  ACI537_RS00865 (ACI537_00865) - 184080..184832 (-) 753 WP_136939425.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI537_RS00870 (ACI537_00870) pilT 185028..186059 (+) 1032 WP_084209471.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI537_RS00875 (ACI537_00875) pilU 186264..187418 (+) 1155 WP_077379436.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI537_RS00880 (ACI537_00880) - 187525..188682 (-) 1158 WP_404983413.1 anhydro-N-acetylmuramic acid kinase -
  ACI537_RS00885 (ACI537_00885) tyrS 188866..190074 (+) 1209 WP_084209468.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37655.28 Da        Isoelectric Point: 6.0550

>NTDB_id=1072224 ACI537_RS00870 WP_084209471.1 185028..186059(+) (pilT) [Cobetia marina strain KMM 1449]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINVPSMDDKQVRALIYEIMNDKQRRDFEEFLETDFSFEVPGVSR
FRVNAFHQGRGAGAVFRTIPSEVLTMEDLGLGEAFRKMCMLPRGLILVTGPTGSGKSTTLAAMIDYINENKYDHILTIED
PVEFVHQSKRCLVNQREVHRDTHGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSATKTVDRI
IDVFPGEEKAMVRSMLSESLQGVIAQTLLKKVGGGRVAAHEIMLATPAIRNLIREDKVAQMYSSIQTGGALGMQTLDSAL
TRLLAEGLVTSEDAQAKAKGLLG

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=1072224 ACI537_RS00870 WP_084209471.1 185028..186059(+) (pilT) [Cobetia marina strain KMM 1449]
ATGGATATTACCGAACTGCTGGCTTTTTCGGCAAAACAGAACGCATCGGATCTGCACCTGTCCGCCGGCCTGCCACCGAT
GATTCGCGTCGATGGTGACGTACGACGCATCAACGTCCCTTCGATGGATGACAAGCAGGTGCGTGCGCTGATCTACGAGA
TCATGAACGACAAGCAGCGTCGCGACTTCGAGGAGTTCCTCGAGACGGATTTCTCCTTCGAGGTGCCGGGCGTCTCGCGC
TTCCGTGTCAATGCTTTCCATCAGGGGCGGGGGGCGGGTGCCGTCTTCCGTACCATCCCGTCGGAAGTGCTGACGATGGA
AGATCTGGGTCTCGGCGAGGCGTTTCGCAAGATGTGCATGCTGCCGCGTGGCCTGATTCTGGTCACCGGTCCGACGGGGT
CGGGCAAGTCGACCACGCTGGCGGCAATGATCGATTACATCAACGAGAACAAGTACGACCACATTCTCACCATCGAGGAC
CCGGTCGAGTTCGTCCACCAGTCCAAGCGTTGCCTGGTCAACCAGCGTGAAGTGCATCGTGATACCCACGGCTTCGCGCC
GGCTCTGCGTTCGGCACTGCGTGAGGACCCGGACGTCATTCTGGTCGGCGAGCTGCGAGATCTCGAGACCATCCGTCTGG
CACTGACGGCCGCCGAGACCGGGCACCTGGTCTTCGGCACCCTTCACACCACCTCGGCGACCAAGACCGTCGACCGCATC
ATCGACGTCTTCCCGGGCGAAGAGAAAGCGATGGTGCGTTCGATGCTGTCCGAATCCCTGCAAGGGGTCATCGCGCAGAC
GCTGCTCAAGAAGGTGGGCGGCGGGCGCGTGGCAGCGCACGAGATCATGCTTGCCACTCCTGCCATCCGCAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCCTCCATCCAGACAGGTGGTGCGTTGGGGATGCAGACGCTGGATTCGGCGCTG
ACGCGACTGTTGGCCGAGGGGCTGGTGACCAGCGAAGACGCGCAAGCGAAGGCCAAGGGGCTGCTGGGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.071

98.834

0.831
  pilT Pseudomonas aeruginosa PAK

82.006

98.834

0.81
  pilT Acinetobacter baumannii D1279779

78.171

98.834

0.773
  pilT Acinetobacter baumannii strain A118

78.171

98.834

0.773
  pilT Acinetobacter nosocomialis M2

77.876

98.834

0.77
  pilT Acinetobacter baylyi ADP1

77.581

98.834

0.767
  pilT Legionella pneumophila strain Lp02

72.781

98.542

0.717
  pilT Legionella pneumophila strain ERS1305867

72.781

98.542

0.717
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

98.251

0.694
  pilT Vibrio cholerae strain A1552

70.623

98.251

0.694
  pilT Neisseria meningitidis 8013

65.689

99.417

0.653
  pilT Neisseria gonorrhoeae MS11

65.396

99.417

0.65
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.622

98.834

0.51
  pilU Pseudomonas stutzeri DSM 10701

42.985

97.668

0.42
  pilU Vibrio cholerae strain A1552

41.493

97.668

0.405
  pilU Acinetobacter baylyi ADP1

39.412

99.125

0.391

Multiple sequence alignment