Detailed information NTG1072225

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACI537_RS00875
• Genome accession: NZ_CP173426
• Coordinates: 186264..187418 (+)
• Length: 384 a.a.
• NCBI ID: WP_077379436.1
• Uniprot ID: -
• Organism: Cobetia marina strain KMM 1449
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 181264..192418

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI537_RS00855 (ACI537_00855)	-	182295..182873 (-)	579	WP_077379444.1	YggT family protein	-
ACI537_RS00860 (ACI537_00860)	proC	183043..183885 (-)	843	WP_136939426.1	pyrroline-5-carboxylate reductase	-
ACI537_RS00865 (ACI537_00865)	-	184080..184832 (-)	753	WP_136939425.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACI537_RS00870 (ACI537_00870)	pilT	185028..186059 (+)	1032	WP_084209471.1	type IV pilus twitching motility protein PilT	Machinery gene
ACI537_RS00875 (ACI537_00875)	pilU	186264..187418 (+)	1155	WP_077379436.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACI537_RS00880 (ACI537_00880)	-	187525..188682 (-)	1158	WP_404983413.1	anhydro-N-acetylmuramic acid kinase	-
ACI537_RS00885 (ACI537_00885)	tyrS	188866..190074 (+)	1209	WP_084209468.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 43034.29 Da
• Isoelectric Point: 6.2727

>NTDB_id=1072225 ACI537_RS00875 WP_077379436.1 186264..187418(+) (pilU) [Cobetia marina strain KMM 1449]
MTPHEWLQELLQLMVSKGSSDLFISTGTPPQMKVNGRMAALGDKKLGVDQVRELVLAPMSDMQRERFEQEREANFAHSLP
GVGRFRISAFYQRSQMGMVIRRIQLSIPSLEELRLPEIIKSLSETKRGLVIFVGGTGAGKSTSLASMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHKSGNGAQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQQGM
MTFDQSLYALHQQDLITEEVALAHADSANDLRLMIKFGDSDSAQEAQLDVMNAASRFSLQDDDG

Nucleotide

• Length: 1155 bp

>NTDB_id=1072225 ACI537_RS00875 WP_077379436.1 186264..187418(+) (pilU) [Cobetia marina strain KMM 1449]
ATGACTCCTCATGAATGGCTGCAGGAACTCCTGCAGTTGATGGTCAGCAAGGGCAGCTCGGATCTTTTCATCTCGACGGG
GACTCCGCCGCAGATGAAGGTCAATGGGCGAATGGCGGCGCTGGGTGACAAGAAGCTCGGTGTCGATCAGGTGCGAGAAC
TGGTGCTTGCGCCGATGAGCGACATGCAGCGAGAGCGCTTCGAACAGGAGCGTGAGGCCAATTTTGCCCATAGTTTGCCG
GGGGTGGGGCGTTTCCGGATCAGCGCCTTCTATCAGCGTAGCCAGATGGGCATGGTCATCCGTCGCATCCAGCTGTCGAT
TCCCAGCCTGGAAGAACTGCGCCTGCCGGAAATCATCAAGAGTCTCTCGGAGACCAAGCGTGGTCTGGTGATCTTCGTCG
GGGGCACGGGTGCCGGCAAGTCGACCTCGCTGGCCTCGATGATCCAGCACCGCAACCAGACCTCAAGTGGCCATATCATC
TGCATCGAGGACCCGATCGAGTACATCCATCCCCATCAGCGCTCCATCGTGACCCAGCGCGAAGTGGGGATCGACACCGA
GTCCTTCGAGGTGGCGCTGCGCAATACGCTGCGTCAGGCACCGGACGTGATCATGATCGGCGAGATCCGTTCGCGTGAGA
CCATGGAGCATGCGCTGACCTTTGCGGAAACCGGTCACCTGTGTCTGGCGACCTTGCACGCCAACAACGCCAACCAGGCG
CTGGATCGCATCATCCACTTCTTCCCGGAAGAGCGTCACGAACAGGTATGGATGGACCTGTCACTGAATCTCAAGGGCAT
CGTTGCCCAGCAGCTACTGCCTCACAAGAGTGGCAATGGCGCACAGCGAGTGCCGGCCATCGAGGTGATGCTGCGCTCGC
CGCTGATCGTCGATCTGATCCGCAAGGGGGCGGTGGTGGAGATAAAGGACGTGATGAAGCGCTCCCAGCAACAGGGCATG
ATGACCTTCGATCAATCGCTCTATGCGTTGCATCAGCAGGACCTGATCACCGAGGAAGTGGCATTGGCGCATGCCGATTC
CGCCAATGACCTGCGTCTGATGATCAAGTTCGGCGATTCCGACAGTGCTCAGGAAGCGCAGCTGGATGTGATGAATGCCG
CCAGTCGCTTCTCGCTGCAGGACGACGATGGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.538	98.177	0.604
pilU	Vibrio cholerae strain A1552	56.911	96.094	0.547
pilU	Acinetobacter baylyi ADP1	55.932	92.188	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.348	89.844	0.398
pilT	Neisseria meningitidis 8013	40.29	89.844	0.362