Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACINK7_RS00800 Genome accession   NZ_CP173170
Coordinates   172117..173154 (+) Length   345 a.a.
NCBI ID   WP_404359154.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 167117..178154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACINK7_RS00775 (ACINK7_00775) - 168149..169456 (-) 1308 WP_404361394.1 selenium-binding protein SBP56-related protein -
  ACINK7_RS00780 (ACINK7_00780) - 169631..169906 (-) 276 WP_404359146.1 DUF4242 domain-containing protein -
  ACINK7_RS00785 (ACINK7_00785) - 169958..170554 (-) 597 WP_404359148.1 TetR family transcriptional regulator C-terminal domain-containing protein -
  ACINK7_RS00790 (ACINK7_00790) - 170809..171072 (+) 264 WP_404359150.1 hypothetical protein -
  ACINK7_RS00795 (ACINK7_00795) - 171091..171774 (-) 684 WP_404359152.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACINK7_RS00800 (ACINK7_00800) pilT 172117..173154 (+) 1038 WP_404359154.1 type IV pilus twitching motility protein PilT Machinery gene
  ACINK7_RS00805 (ACINK7_00805) pilU 173167..174300 (+) 1134 WP_404359156.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACINK7_RS00810 (ACINK7_00810) djlA 174306..175115 (-) 810 WP_404359158.1 co-chaperone DjlA -
  ACINK7_RS00815 (ACINK7_00815) - 175190..176089 (+) 900 WP_404359160.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  ACINK7_RS00820 (ACINK7_00820) radC 176465..177139 (-) 675 WP_404359162.1 DNA repair protein RadC -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38313.15 Da        Isoelectric Point: 6.8402

>NTDB_id=1070384 ACINK7_RS00800 WP_404359154.1 172117..173154(+) (pilT) [Methylotuvimicrobium sp. KM1]
MDIAELLTFSVKNNASDLHLSAGLPPMIRVDGDIRRINIPALEHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLSLEDLNAPKFFEEVTRKPRGLILVTGPTGSGKSTTLAAMVNHINTNDYAHILTVED
PIEFVHESQKCLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTAAIRNLIREAKVAQMYSAIQTGRKEGMQTLDQNL
KELVEKGLVASKVAMLKAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1070384 ACINK7_RS00800 WP_404359154.1 172117..173154(+) (pilT) [Methylotuvimicrobium sp. KM1]
ATGGATATTGCTGAATTATTGACTTTTTCCGTTAAAAATAATGCATCGGATTTACATTTATCGGCCGGTTTGCCGCCGAT
GATTCGGGTGGACGGCGATATCCGGCGTATCAATATTCCTGCCTTAGAGCATAAAGAAGTTCATGCCTTGATTTACGACA
TCATGAACGATAAACAGCGCCGCGATTATGAAGAATTTCTTGAAACCGACTTTTCTTTTGCATTGCCGGGTGTTGCAAGG
TTTCGTGTCAATGCATTCAATCAGGATCGAGGTGCGGGGGCGGTTTTTAGAACGATTCCTTCGAAGGTGTTGAGTTTAGA
AGATCTTAATGCGCCAAAGTTTTTTGAAGAAGTTACGCGCAAACCGAGAGGTTTAATTCTGGTAACAGGACCTACCGGAT
CGGGAAAGTCAACGACGCTGGCGGCGATGGTCAACCATATCAACACCAATGATTATGCACATATTTTAACCGTTGAAGAC
CCGATCGAGTTCGTCCATGAAAGCCAAAAATGCTTGATCAACCAACGAGAAGTCCATCGCGATACATTAGGTTTCAACGA
GGCCTTGCGATCCGCGTTGCGGGAAGACCCCGATGTGATTCTAGTGGGCGAAATGCGCGACTTGGAAACGATACGATTGG
CGCTGACCGCGGCCGAAACCGGTCACTTGGTTTTCGGCACGTTGCACACCACGTCGGCCGCGAAAACGATCGACCGTATT
ATCGATGTGTTTCCGGCGGCTGAAAAAGACATGATCCGCTCGATGTTGTCGGAATCGTTGCAAGCGGTCATCTCGCAAAC
CTTACTGAAAAAAGTCGGTGGAGGACGTATCGCGGCCCATGAAATCATGGTCGGGACTGCAGCCATTAGAAACTTGATTC
GCGAAGCAAAAGTCGCGCAAATGTATTCGGCGATTCAAACCGGGCGCAAGGAAGGCATGCAGACCTTGGATCAAAATTTG
AAAGAATTGGTTGAAAAAGGCTTAGTGGCTTCAAAAGTCGCCATGTTGAAAGCGGTTAACAAAGATATGTTCAGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

80.178

97.971

0.786
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Legionella pneumophila strain ERS1305867

72.965

99.71

0.728
  pilT Legionella pneumophila strain Lp02

72.965

99.71

0.728
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.084

96.232

0.684
  pilT Vibrio cholerae strain A1552

71.084

96.232

0.684
  pilT Neisseria meningitidis 8013

66.764

99.42

0.664
  pilT Neisseria gonorrhoeae MS11

66.181

99.42

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Acinetobacter baylyi ADP1

39.769

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.306

100

0.394
  pilU Vibrio cholerae strain A1552

40

94.203

0.377

Multiple sequence alignment