Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACINK7_RS00805 Genome accession   NZ_CP173170
Coordinates   173167..174300 (+) Length   377 a.a.
NCBI ID   WP_404359156.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 168167..179300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACINK7_RS00780 (ACINK7_00780) - 169631..169906 (-) 276 WP_404359146.1 DUF4242 domain-containing protein -
  ACINK7_RS00785 (ACINK7_00785) - 169958..170554 (-) 597 WP_404359148.1 TetR family transcriptional regulator C-terminal domain-containing protein -
  ACINK7_RS00790 (ACINK7_00790) - 170809..171072 (+) 264 WP_404359150.1 hypothetical protein -
  ACINK7_RS00795 (ACINK7_00795) - 171091..171774 (-) 684 WP_404359152.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACINK7_RS00800 (ACINK7_00800) pilT 172117..173154 (+) 1038 WP_404359154.1 type IV pilus twitching motility protein PilT Machinery gene
  ACINK7_RS00805 (ACINK7_00805) pilU 173167..174300 (+) 1134 WP_404359156.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACINK7_RS00810 (ACINK7_00810) djlA 174306..175115 (-) 810 WP_404359158.1 co-chaperone DjlA -
  ACINK7_RS00815 (ACINK7_00815) - 175190..176089 (+) 900 WP_404359160.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  ACINK7_RS00820 (ACINK7_00820) radC 176465..177139 (-) 675 WP_404359162.1 DNA repair protein RadC -
  ACINK7_RS00825 (ACINK7_00825) coaBC 177574..178791 (+) 1218 WP_404359164.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ACINK7_RS00830 (ACINK7_00830) dut 178760..179215 (+) 456 WP_404359166.1 dUTP diphosphatase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42566.85 Da        Isoelectric Point: 6.4444

>NTDB_id=1070385 ACINK7_RS00805 WP_404359156.1 173167..174300(+) (pilU) [Methylotuvimicrobium sp. KM1]
MDEFKRLLELMVQQKASDLFITAGRPPTIKVDGKLIEVSKTMLNNDQSRAIVMSVMTQRQKDEFDNTKECQFAIAFPKLG
RFRVSAFTQRDAAGMVLRRIETHIPDAEDLHLPPVLKDLVMNKRGLVLFVGGTGTGKSTSLASLIKYRNQNSSGHIITIE
DPLEFLHPHLGCIVTQREVGMDTESYEVALKNTLRQAPDVILIGEVRTRETMQNAITFAETGHLCITTLHANNANQALDR
ILHFFPDEMHNQLFMDLSLNLRGIVAQQLIARADGKGRYPAVEILLNTPLVSDLIRKGEVHKLKELMKNSREHGMQTFDQ
ALYDLYTAGKISYEDALNSADSRNEVRLMIKLGAEDLGTLVNENMHLKEDEDEHTRF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1070385 ACINK7_RS00805 WP_404359156.1 173167..174300(+) (pilU) [Methylotuvimicrobium sp. KM1]
ATGGATGAATTTAAGCGACTACTTGAGTTAATGGTTCAGCAAAAGGCCTCCGATTTATTCATCACAGCGGGGCGCCCGCC
CACGATAAAAGTCGACGGTAAGTTGATCGAAGTATCCAAAACGATGCTAAACAACGATCAATCTCGAGCCATTGTCATGA
GTGTGATGACGCAACGGCAAAAAGACGAATTCGATAATACCAAAGAGTGTCAGTTTGCGATTGCTTTTCCTAAGCTCGGT
AGATTCCGGGTGAGTGCGTTTACCCAGCGAGATGCTGCGGGAATGGTGTTGAGGCGCATCGAGACTCATATTCCCGATGC
CGAAGATCTTCATTTGCCGCCGGTTTTAAAAGATTTGGTCATGAATAAGCGAGGACTGGTGTTGTTCGTCGGCGGGACGG
GAACCGGTAAGTCGACTTCATTGGCGTCGTTGATCAAATACCGAAACCAAAATAGCAGCGGACATATTATTACCATTGAA
GATCCGCTCGAGTTTTTGCATCCGCATTTGGGTTGCATTGTCACGCAGCGCGAAGTCGGCATGGATACCGAATCCTATGA
AGTCGCACTTAAGAATACCTTGCGCCAAGCGCCTGATGTTATCCTGATCGGTGAGGTTAGAACCCGTGAAACGATGCAAA
ATGCGATTACGTTCGCCGAAACCGGGCATTTGTGTATAACGACCTTGCATGCGAACAATGCCAATCAGGCATTGGATCGG
ATTTTGCATTTTTTCCCGGACGAAATGCATAATCAATTATTTATGGATTTGTCGCTGAATTTGCGAGGCATTGTCGCGCA
GCAGTTGATTGCCCGTGCCGATGGCAAAGGGCGCTATCCCGCAGTTGAAATTTTGTTGAACACACCCTTGGTTTCCGATT
TGATTCGTAAAGGCGAAGTTCACAAACTGAAGGAGTTAATGAAGAATTCGCGGGAACATGGTATGCAGACATTCGATCAA
GCGCTTTACGATCTCTATACAGCCGGAAAAATTAGTTATGAAGATGCCTTAAACTCTGCGGACTCAAGAAATGAAGTGCG
CTTGATGATTAAACTGGGTGCCGAAGACTTAGGGACTTTAGTCAATGAAAATATGCATCTAAAAGAAGACGAAGATGAGC
ATACTCGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.517

100

0.655
  pilU Acinetobacter baylyi ADP1

63.277

93.899

0.594
  pilU Vibrio cholerae strain A1552

58.166

92.573

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

90.981

0.39
  pilT Legionella pneumophila strain Lp02

42.012

89.655

0.377
  pilT Legionella pneumophila strain ERS1305867

42.012

89.655

0.377

Multiple sequence alignment