Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACH784_RS04620 Genome accession   NZ_CP173161
Coordinates   977645..978691 (+) Length   348 a.a.
NCBI ID   WP_404393823.1    Uniprot ID   -
Organism   Pseudoalteromonas phenolica strain a1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 972645..983691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH784_RS04590 (ACH784_04590) hemW 973371..974507 (-) 1137 WP_404393817.1 radical SAM family heme chaperone HemW -
  ACH784_RS04595 (ACH784_04595) - 974510..975097 (-) 588 WP_404393818.1 XTP/dITP diphosphatase -
  ACH784_RS04600 (ACH784_04600) - 975105..975539 (-) 435 WP_404393819.1 DUF4426 domain-containing protein -
  ACH784_RS04605 (ACH784_04605) - 975552..976085 (-) 534 WP_404393820.1 YggT family protein -
  ACH784_RS04610 (ACH784_04610) proC 976095..976916 (-) 822 WP_404393821.1 pyrroline-5-carboxylate reductase -
  ACH784_RS04615 (ACH784_04615) - 976931..977614 (-) 684 WP_404393822.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACH784_RS04620 (ACH784_04620) pilT 977645..978691 (+) 1047 WP_404393823.1 type IV pilus twitching motility protein PilT Machinery gene
  ACH784_RS04625 (ACH784_04625) pilU 978703..979821 (+) 1119 WP_404393824.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACH784_RS04630 (ACH784_04630) - 979799..980209 (+) 411 WP_404393825.1 hypothetical protein -
  ACH784_RS04635 (ACH784_04635) - 980308..980715 (+) 408 WP_404393826.1 hypothetical protein -
  ACH784_RS04640 (ACH784_04640) ruvX 980730..981179 (-) 450 WP_404393827.1 Holliday junction resolvase RuvX -
  ACH784_RS04645 (ACH784_04645) - 981195..981755 (-) 561 WP_404393828.1 YqgE/AlgH family protein -
  ACH784_RS04650 (ACH784_04650) gshB 981803..982759 (-) 957 WP_404393829.1 glutathione synthase -
  ACH784_RS04655 (ACH784_04655) rsmE 982815..983549 (-) 735 WP_404393830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38453.87 Da        Isoelectric Point: 6.4417

>NTDB_id=1070335 ACH784_RS04620 WP_404393823.1 977645..978691(+) (pilT) [Pseudoalteromonas phenolica strain a1]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRVNIPALESKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNTSRGPAAVFRTIPSTVLSLDDLGAPDIFRDISDNPRGLVLVTGPTGSGKSTTLAAMVDYINNSKHHHILTIED
PIEFVHQNKLSLINQREVHRDTHSFKAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLQAVISQTLVKKVGGGRVAAHEIMLGIPAIRNLIREDKIAQMYSAIQTGAMHGMQTMDQCL
KNLVNRGLITAQDAQSKAHDKAQFGPNM

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=1070335 ACH784_RS04620 WP_404393823.1 977645..978691(+) (pilT) [Pseudoalteromonas phenolica strain a1]
ATGGATATTACTGAATTATTGGCCTTTAGTGTTCAACATAAAGCCTCGGATTTACATTTATCATCGGGGGTTTCACCGAT
GATCCGGGTTGACGGCGATGTGCGTCGTGTAAATATTCCCGCATTAGAATCGAAAGACGTAAATAGCCTCGTTTACGATA
TTATGAATGATAACCAGCGAAAGGACTATGAGCAAAATTTAGAGGTGGATTTTTCTTTTGAAGTGCCTAATTTAGCGCGT
TTCCGTGTTAATGCCTTCAACACCAGTCGTGGTCCTGCAGCGGTATTTCGTACTATTCCTTCAACGGTTCTGTCTTTAGA
TGATTTAGGTGCGCCAGATATTTTTAGAGATATCTCAGATAACCCTCGAGGTTTAGTACTGGTTACTGGGCCGACAGGCT
CTGGTAAATCAACCACCTTAGCTGCGATGGTCGATTATATTAATAACAGCAAACATCACCACATTCTAACCATCGAAGAC
CCAATTGAATTTGTACATCAAAATAAACTCAGTCTTATTAACCAGCGTGAAGTGCATAGAGATACACACAGCTTTAAAGC
GGCCCTTCGCTCTGCGCTGCGTGAAGACCCTGATGTTATTCTAGTGGGTGAATTACGTGATTTAGAGACCATTCGTCTAG
CTATGACAGCAGCTGAAACAGGTCATTTAGTGTTTGGTACTTTGCACACCACATCAGCACCCAAAACCATTGACCGTATT
ATCGATGTATTCCCGGGTGAAGAAAAAGACATGGTGCGCTCAATGTTATCTGAATCACTGCAAGCGGTTATTTCTCAAAC
ATTGGTTAAAAAAGTAGGTGGGGGTCGTGTGGCAGCACACGAAATTATGCTCGGTATTCCGGCAATTCGTAACCTTATCC
GTGAAGATAAAATTGCCCAGATGTACTCGGCAATTCAAACCGGTGCCATGCATGGTATGCAAACCATGGATCAGTGTTTA
AAGAATTTGGTTAACCGTGGCCTTATTACGGCACAAGATGCGCAATCTAAAGCGCACGATAAAGCACAATTTGGTCCAAA
CATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.907

98.851

0.77
  pilT Acinetobacter baumannii strain A118

77.907

98.851

0.77
  pilT Acinetobacter nosocomialis M2

77.907

98.851

0.77
  pilT Acinetobacter baylyi ADP1

76.163

98.851

0.753
  pilT Pseudomonas stutzeri DSM 10701

75.872

98.851

0.75
  pilT Pseudomonas aeruginosa PAK

75.291

98.851

0.744
  pilT Legionella pneumophila strain Lp02

72.674

98.851

0.718
  pilT Legionella pneumophila strain ERS1305867

72.674

98.851

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

96.839

0.704
  pilT Vibrio cholerae strain A1552

72.7

96.839

0.704
  pilT Neisseria gonorrhoeae MS11

70.088

97.989

0.687
  pilT Neisseria meningitidis 8013

70.088

97.989

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

99.138

0.523
  pilU Vibrio cholerae strain A1552

41.493

96.264

0.399
  pilU Pseudomonas stutzeri DSM 10701

40

96.264

0.385
  pilU Acinetobacter baylyi ADP1

38.824

97.701

0.379

Multiple sequence alignment