Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACH784_RS04625 Genome accession   NZ_CP173161
Coordinates   978703..979821 (+) Length   372 a.a.
NCBI ID   WP_404393824.1    Uniprot ID   -
Organism   Pseudoalteromonas phenolica strain a1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 973703..984821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH784_RS04595 (ACH784_04595) - 974510..975097 (-) 588 WP_404393818.1 XTP/dITP diphosphatase -
  ACH784_RS04600 (ACH784_04600) - 975105..975539 (-) 435 WP_404393819.1 DUF4426 domain-containing protein -
  ACH784_RS04605 (ACH784_04605) - 975552..976085 (-) 534 WP_404393820.1 YggT family protein -
  ACH784_RS04610 (ACH784_04610) proC 976095..976916 (-) 822 WP_404393821.1 pyrroline-5-carboxylate reductase -
  ACH784_RS04615 (ACH784_04615) - 976931..977614 (-) 684 WP_404393822.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACH784_RS04620 (ACH784_04620) pilT 977645..978691 (+) 1047 WP_404393823.1 type IV pilus twitching motility protein PilT Machinery gene
  ACH784_RS04625 (ACH784_04625) pilU 978703..979821 (+) 1119 WP_404393824.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACH784_RS04630 (ACH784_04630) - 979799..980209 (+) 411 WP_404393825.1 hypothetical protein -
  ACH784_RS04635 (ACH784_04635) - 980308..980715 (+) 408 WP_404393826.1 hypothetical protein -
  ACH784_RS04640 (ACH784_04640) ruvX 980730..981179 (-) 450 WP_404393827.1 Holliday junction resolvase RuvX -
  ACH784_RS04645 (ACH784_04645) - 981195..981755 (-) 561 WP_404393828.1 YqgE/AlgH family protein -
  ACH784_RS04650 (ACH784_04650) gshB 981803..982759 (-) 957 WP_404393829.1 glutathione synthase -
  ACH784_RS04655 (ACH784_04655) rsmE 982815..983549 (-) 735 WP_404393830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACH784_RS04660 (ACH784_04660) - 983607..984083 (-) 477 WP_404393831.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41360.33 Da        Isoelectric Point: 5.4925

>NTDB_id=1070336 ACH784_RS04625 WP_404393824.1 978703..979821(+) (pilU) [Pseudoalteromonas phenolica strain a1]
MLLNQFLQAMVDRQASDLFVSAGLAVSAKIDGELTPLSEERLTPEASLEIVESAMNDRQKEEFYREKECNFAIATDEGRF
RVSAFWQRDCAGMVLRRIVTQIPDVNDLGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIEDP
IEFVHEHKKSIITQREVGIDTESFEAALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRIM
HLVPKEKHDKLKYDLALNLRAIVAQQLVPSAKGEGREAAIEILLNSPMVAELIKKGDIGSIKETMAKSKEMGMQTFDQAL
FELYKQRRINYADALHYADSPNDLRLMIKLQNNEQQGAGFLQGVTVDGLDSK

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1070336 ACH784_RS04625 WP_404393824.1 978703..979821(+) (pilU) [Pseudoalteromonas phenolica strain a1]
ATGCTACTTAATCAATTCTTACAAGCCATGGTTGACCGACAAGCATCAGATTTATTTGTCTCTGCTGGTCTTGCTGTCAG
TGCCAAAATTGATGGGGAATTAACGCCTTTAAGCGAAGAGCGTTTAACACCTGAAGCCTCTCTGGAGATCGTAGAGTCTG
CGATGAATGACAGGCAAAAAGAAGAGTTCTATCGCGAAAAAGAATGTAATTTTGCGATTGCCACAGATGAAGGACGTTTC
CGTGTTTCTGCATTTTGGCAAAGAGATTGTGCAGGTATGGTATTGCGTCGCATTGTGACGCAAATTCCCGATGTGAATGA
TTTGGGTTTACCTTCAGTTTTGACGGATGTGATCATGTCAAAACGCGGCTTAGTGCTGTTTGTGGGTGGTACAGGTACGG
GTAAATCAACATCACTAGCAGCACTGCTTGGCTATAGAAACCGTAATCAGCGTGGTCATATTCTAACAATTGAAGATCCA
ATTGAATTCGTGCATGAGCACAAAAAAAGCATTATCACGCAACGTGAAGTAGGTATTGATACAGAGAGCTTTGAAGCCGC
GCTAAAGAGCTCTTTGCGACAAGCTCCAGATGTTATTTTAATTGGTGAAATTCGTTCTCAAGAAACTATGGAATATGCAC
TTAGCTTTGCTGAAACAGGCCATTTATGTGTGGCAACACTGCACGCAAATAACGCTAACCAAGCGATTGACCGTATTATG
CACCTGGTTCCGAAAGAGAAACATGATAAGCTTAAATATGACTTAGCTTTGAATCTGCGTGCCATTGTTGCTCAGCAACT
CGTGCCTTCAGCGAAAGGGGAAGGGCGTGAAGCAGCCATCGAGATTTTACTTAACTCGCCAATGGTGGCTGAGCTTATCA
AAAAAGGTGATATCGGCTCGATAAAAGAAACCATGGCAAAGTCTAAAGAAATGGGTATGCAAACTTTTGATCAGGCCTTG
TTTGAGCTCTATAAACAACGTCGTATTAATTATGCAGACGCACTTCACTACGCAGACTCGCCAAATGATCTACGCTTAAT
GATAAAGCTGCAGAATAATGAGCAGCAAGGTGCAGGTTTCTTACAAGGTGTGACTGTTGATGGGTTGGATTCAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.373

100

0.575
  pilU Acinetobacter baylyi ADP1

54.907

100

0.556
  pilU Vibrio cholerae strain A1552

56.403

98.656

0.556
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.737

96.237

0.411
  pilT Legionella pneumophila strain Lp02

42.169

89.247

0.376
  pilT Legionella pneumophila strain ERS1305867

42.169

89.247

0.376
  pilT Acinetobacter baylyi ADP1

40.351

91.935

0.371
  pilT Acinetobacter nosocomialis M2

39.766

91.935

0.366
  pilT Acinetobacter baumannii D1279779

39.766

91.935

0.366
  pilT Acinetobacter baumannii strain A118

39.766

91.935

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.006

85.753

0.36
  pilT Vibrio cholerae strain A1552

42.006

85.753

0.36

Multiple sequence alignment