Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACIHQS_RS11635 Genome accession   NZ_CP173025
Coordinates   2461279..2462316 (+) Length   345 a.a.
NCBI ID   WP_004881041.1    Uniprot ID   G7GDK1
Organism   Acinetobacter venetianus strain AVYS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2456279..2467316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIHQS_RS11625 (ACIHQS_11625) - 2459620..2460273 (-) 654 WP_004881037.1 hypothetical protein -
  ACIHQS_RS11630 (ACIHQS_11630) - 2460475..2461152 (-) 678 WP_404228041.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACIHQS_RS11635 (ACIHQS_11635) pilT 2461279..2462316 (+) 1038 WP_004881041.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIHQS_RS11640 (ACIHQS_11640) pilU 2462341..2463459 (+) 1119 WP_004881042.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIHQS_RS11645 (ACIHQS_11645) fur 2463556..2463993 (-) 438 WP_004881044.1 ferric iron uptake transcriptional regulator -
  ACIHQS_RS11650 (ACIHQS_11650) - 2464105..2464503 (+) 399 WP_004881046.1 outer membrane protein assembly factor BamE -
  ACIHQS_RS11655 (ACIHQS_11655) - 2464558..2464884 (-) 327 WP_004881047.1 RnfH family protein -
  ACIHQS_RS11660 (ACIHQS_11660) - 2464884..2465966 (-) 1083 WP_404228046.1 hypothetical protein -
  ACIHQS_RS11665 (ACIHQS_11665) - 2466224..2466679 (-) 456 WP_004881051.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38356.96 Da        Isoelectric Point: 6.3479

>NTDB_id=1070000 ACIHQS_RS11635 WP_004881041.1 2461279..2462316(+) (pilT) [Acinetobacter venetianus strain AVYS1]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDFEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGSIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGLISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1070000 ACIHQS_RS11635 WP_004881041.1 2461279..2462316(+) (pilT) [Acinetobacter venetianus strain AVYS1]
ATGGATATTACAGAATTACTCGCCTTTTCAGTAAAAAACGGTGCTTCAGATTTACACCTTTCTGCGGGTATGCCGCCTAT
GATTCGTGTAGATGGTGAAGTTCGTCGTATTAACTTACCTGCATTAGAACATAAAGATGTACACCGTTTGGTGTATGACA
TTATGAATGACAAACAACGTCGTGATTTTGAAGAAGATCTAGAAACAGACTTTTCATTTGAAGTACCAAATATCGCTCGT
TTCCGTGTGAACGCATTCAACCAAAACCGCGGTGCTGGTGCAGTATTCCGTACCATTCCTTCAAAAGTTTTAACGATGGA
AGATTTAGGATTGGGATCAATTTTCAAAGAAATTTGTGACTATCCACGTGGAATTGTACTTGTAACAGGTCCAACAGGTT
CGGGTAAATCAACCACCTTGGCTGCGATGATCGACTATATTAATGAAAATCGCTATGACCATATCTTAACTGTCGAAGAC
CCAATCGAATTTGTACACCAATCAAAAAAATGTTTGATTAACCAACGTGAAGTTCATCGTGATACACATGGATTCAATGA
AGCACTTCGTTCTGCGCTCCGTGAAGACCCTGATATTATCTTGGTCGGTGAGATGCGTGACTTAGAGACCATCCGCCTCG
CTTTAACTGCAGCCGAAACAGGTCACTTAGTATTTGGAACGCTACATACCACCTCAGCAGCAAAAACCATTGACCGTGTG
ATCGATGTCTTCCCTGCTGAAGAAAAAGACATGGTACGTGCCATGTTGTCAGAATCGTTACAAGCCGTTATTTCTCAAAC
ACTATTGAAGAAAAATGGTGGCGGTCGTGTTGCTGCACATGAGATTATGATTGGTATTCCTGCGATTCGTAACCTTGTAC
GTGAAAACAAAGTTGCACAAATGTACTCAGCGATTCAGACGGGTGCTAACTATGGTATGACAACGCTAGATCAAAGCCTG
AAAACCTTAGTTTCTAAGGGATTAATTAGCCCTCAAACAGCCCGTACTGTTGCTAAACAACCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7GDK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.522

100

0.965
  pilT Acinetobacter baumannii D1279779

96.232

100

0.962
  pilT Acinetobacter baumannii strain A118

96.232

100

0.962
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394

Multiple sequence alignment