Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACIHQS_RS11640 Genome accession   NZ_CP173025
Coordinates   2462341..2463459 (+) Length   372 a.a.
NCBI ID   WP_004881042.1    Uniprot ID   G7GDK0
Organism   Acinetobacter venetianus strain AVYS1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2457341..2468459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIHQS_RS11625 (ACIHQS_11625) - 2459620..2460273 (-) 654 WP_004881037.1 hypothetical protein -
  ACIHQS_RS11630 (ACIHQS_11630) - 2460475..2461152 (-) 678 WP_404228041.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACIHQS_RS11635 (ACIHQS_11635) pilT 2461279..2462316 (+) 1038 WP_004881041.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIHQS_RS11640 (ACIHQS_11640) pilU 2462341..2463459 (+) 1119 WP_004881042.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIHQS_RS11645 (ACIHQS_11645) fur 2463556..2463993 (-) 438 WP_004881044.1 ferric iron uptake transcriptional regulator -
  ACIHQS_RS11650 (ACIHQS_11650) - 2464105..2464503 (+) 399 WP_004881046.1 outer membrane protein assembly factor BamE -
  ACIHQS_RS11655 (ACIHQS_11655) - 2464558..2464884 (-) 327 WP_004881047.1 RnfH family protein -
  ACIHQS_RS11660 (ACIHQS_11660) - 2464884..2465966 (-) 1083 WP_404228046.1 hypothetical protein -
  ACIHQS_RS11665 (ACIHQS_11665) - 2466224..2466679 (-) 456 WP_004881051.1 bacteriohemerythrin -
  ACIHQS_RS11670 (ACIHQS_11670) - 2466963..2467811 (-) 849 WP_007481247.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41760.90 Da        Isoelectric Point: 6.2894

>NTDB_id=1070001 ACIHQS_RS11640 WP_004881042.1 2462341..2463459(+) (pilU) [Acinetobacter venetianus strain AVYS1]
MDFNDLLNLMIQQNASDLFVTADVEPSMKINGQIVPVSKTKLSGEIVGQLIQSVMTDKQRKEFAETRECNFAITNREKTA
RFRVSAFQQRDMPGMILRKIETHIPSMEDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGFRNHNSKGHIITIE
DPIEFVHQHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLRALVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGSDQLLSASKNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1070001 ACIHQS_RS11640 WP_004881042.1 2462341..2463459(+) (pilU) [Acinetobacter venetianus strain AVYS1]
ATGGACTTTAATGATTTACTCAATTTGATGATTCAGCAAAATGCATCTGACTTATTTGTCACTGCTGATGTTGAACCATC
AATGAAAATTAATGGTCAGATCGTACCTGTATCAAAAACTAAATTGTCTGGGGAAATTGTTGGTCAACTTATTCAGTCAG
TAATGACTGATAAGCAACGTAAAGAGTTTGCAGAAACTCGTGAATGTAACTTTGCCATTACCAACCGTGAAAAAACTGCA
CGTTTCCGTGTGAGTGCATTTCAGCAACGTGATATGCCTGGCATGATTTTGCGTAAAATTGAAACGCACATCCCGAGCAT
GGAAGATTTAAAATTACCCGAAGTTCTTAAAGAACTTGCAATGACCAAACGCGGTATCATTATTTTTGTTGGCGCAACAG
GTACTGGTAAATCAACTTCTTTAGCATCAATGATTGGCTTCCGTAACCATAATTCGAAAGGCCATATCATTACCATCGAA
GATCCAATTGAATTTGTACACCAACATGCAGGCTGTATCATCACACAGCGTGAAGTCGGTATCGACACAGATTCATTTGA
AGTCGCACTGAAGAACACCTTACGTCAAGCACCAGATGTGATTCTAATTGGTGAGATTCGTTCTCGTGAGACCATGGACT
ATGCGATTGCTTTTGCTGAAACGGGACACTTAGTGCTTGCAACACTACATGCGAACAATGCCAACCAAGCGTTAGATCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAACTTTACATGGACTTGTCTCTAAACCTACGCGCGTTAGTAGCACA
GCAACTTATTCCAACACCTGATGGTAATTCACGTCGCGCTGCAATTGAAATTTTAATTAATACTCCACTCCTTGCAGATT
ACATCCGCAAAGGTGAAATTCATGAGATTAAGGATCTAATGAAACGCTCGCGTGAGCTAGGTATGCAAACCTTCGACCAA
GCGTTGTTTGATCTTTATAAAGCAGGTGAAATTACCTATAAAGATGCGCTTAAACATGCAGACTCTCCAAATGACTTACG
TTTAACCATTAAGTTATCTGAAGAGGGTTCAGACCAATTATTGAGCGCAAGTAAAAATATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7GDK0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.661

100

0.769
  pilU Pseudomonas stutzeri DSM 10701

70.35

99.731

0.702
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.934

97.849

0.401
  pilT Legionella pneumophila strain Lp02

41.896

87.903

0.368
  pilT Legionella pneumophila strain ERS1305867

41.896

87.903

0.368
  pilT Neisseria meningitidis 8013

40.294

91.398

0.368
  pilT Neisseria gonorrhoeae MS11

40.294

91.398

0.368
  pilT Pseudomonas aeruginosa PAK

39.017

93.011

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36

Multiple sequence alignment