Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAM51_RS16390 Genome accession   NZ_AP028672
Coordinates   3574354..3575397 (-) Length   347 a.a.
NCBI ID   WP_010463046.1    Uniprot ID   -
Organism   Acidovorax sp. A79     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3569354..3580397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM51_RS16360 (GmRootA79_32490) - 3569374..3569784 (+) 411 WP_369641205.1 YraN family protein -
  ACAM51_RS16365 (GmRootA79_32500) - 3569884..3570486 (+) 603 WP_218297335.1 SIS domain-containing protein -
  ACAM51_RS16370 (GmRootA79_32510) - 3570483..3571151 (+) 669 WP_369641206.1 BON domain-containing protein -
  ACAM51_RS16375 (GmRootA79_32520) - 3571375..3572289 (-) 915 WP_369641207.1 NAD(P)-dependent oxidoreductase -
  ACAM51_RS16380 (GmRootA79_32530) pilU 3572457..3573593 (-) 1137 WP_218297332.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM51_RS16385 (GmRootA79_32540) - 3573658..3574293 (-) 636 WP_218297331.1 Crp/Fnr family transcriptional regulator -
  ACAM51_RS16390 (GmRootA79_32550) pilT 3574354..3575397 (-) 1044 WP_010463046.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM51_RS16395 (GmRootA79_32560) - 3575434..3576150 (+) 717 WP_218297330.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM51_RS16400 (GmRootA79_32570) ltaE 3576167..3577237 (-) 1071 WP_218297329.1 low-specificity L-threonine aldolase -
  ACAM51_RS16405 (GmRootA79_32580) - 3577431..3578699 (-) 1269 WP_218297328.1 branched-chain amino acid ABC transporter substrate-binding protein -
  ACAM51_RS16410 (GmRootA79_32590) - 3578778..3579758 (-) 981 WP_218297327.1 tripartite tricarboxylate transporter substrate binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38148.92 Da        Isoelectric Point: 6.7743

>NTDB_id=106865 ACAM51_RS16390 WP_010463046.1 3574354..3575397(-) (pilT) [Acidovorax sp. A79]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDSQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=106865 ACAM51_RS16390 WP_010463046.1 3574354..3575397(-) (pilT) [Acidovorax sp. A79]
GTGGACATTACCCAACTGCTCGCTTTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTTTCAGCCGGTCTGCCGCCCAT
GATCCGGGTCCACGGGGATGTCCGCCGCATCAATGTCGATGCGCTCGACCACAAGACGGTGCATGCCATGGTGTACGACA
TCATGAGCGACTCGCAGCGCAAGACGTATGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGAAGGCCTGGCCCGC
TTTCGCGTCAACGCCTTCAACCAGAATCGCGGCGCGGCCGCCGTGTTCCGGACCATTCCCTCCAAGATCCTCACGCTCGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGACCTGGCCCTGAAGCCTCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGCCATGGTCAACTACCTGAACGAAACCGAGTACGGCCACATCCTCACGGTGGAGGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGCCCGATGACGCTGTCGTTCGCCGC
CGCGCTGAAGTCCGCGCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGTCTGG
CCATGACGGCGGCGGAAACGGGCCATTTGGTGTTCGGCACGCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGCCGAAGAAAAGGAAATGGTTCGCGCGATGTTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCTCGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAT
CTCACCGACCTCGTTCGCCGCAACATCATCAGCCCGGCCGAGGCGCGCGCCAAGGCCAAGATCCCCGAAAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment