Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAM51_RS16380 Genome accession   NZ_AP028672
Coordinates   3572457..3573593 (-) Length   378 a.a.
NCBI ID   WP_218297332.1    Uniprot ID   -
Organism   Acidovorax sp. A79     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3567457..3578593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM51_RS16355 (GmRootA79_32480) rsmI 3568421..3569374 (-) 954 WP_218297337.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACAM51_RS16360 (GmRootA79_32490) - 3569374..3569784 (+) 411 WP_369641205.1 YraN family protein -
  ACAM51_RS16365 (GmRootA79_32500) - 3569884..3570486 (+) 603 WP_218297335.1 SIS domain-containing protein -
  ACAM51_RS16370 (GmRootA79_32510) - 3570483..3571151 (+) 669 WP_369641206.1 BON domain-containing protein -
  ACAM51_RS16375 (GmRootA79_32520) - 3571375..3572289 (-) 915 WP_369641207.1 NAD(P)-dependent oxidoreductase -
  ACAM51_RS16380 (GmRootA79_32530) pilU 3572457..3573593 (-) 1137 WP_218297332.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM51_RS16385 (GmRootA79_32540) - 3573658..3574293 (-) 636 WP_218297331.1 Crp/Fnr family transcriptional regulator -
  ACAM51_RS16390 (GmRootA79_32550) pilT 3574354..3575397 (-) 1044 WP_010463046.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM51_RS16395 (GmRootA79_32560) - 3575434..3576150 (+) 717 WP_218297330.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM51_RS16400 (GmRootA79_32570) ltaE 3576167..3577237 (-) 1071 WP_218297329.1 low-specificity L-threonine aldolase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41861.28 Da        Isoelectric Point: 6.9077

>NTDB_id=106864 ACAM51_RS16380 WP_218297332.1 3572457..3573593(-) (pilU) [Acidovorax sp. A79]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTPNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGKVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRGMVSQRLIPKQDGKGRAAAVEIMLNTPLISDLIFKGDVSEIKEIMKKSRNLG
MQTFDQALFDLYEANVISYEDALRNADSLNDLRLQIKLNSQRAKSPDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=106864 ACAM51_RS16380 WP_218297332.1 3572457..3573593(-) (pilU) [Acidovorax sp. A79]
ATGGAACGCGATCAGGCCAGTAAATTCATCAATGACCTGCTCAAGTTGATGGTGAGCCGCAATGGCAGCGACTTGTTCAT
CACGGCGGAATTCCCCCCCGCGATCAAGGTCGATGGCAAGGTGACCAAGGTGTCGCCCCAGCCGCTCACGCCCAACCACA
CGCTCACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCCGATTTCGAACGCACCAAGGAGTGCAACTTCGCGATC
TCGCCCGCCGGCATCGGGCGGTTTCGCGTCAACGCGTTCATCCAGCAGGGCAAGGTCGGCATGGTGCTGCGCACGATTCC
GCTCACGCTGCCCACCATCGACGGCCTGGGCGTGCCGCAGGTGCTGAAAGAAGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAATCGACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAACTCGTTCGGC
CACATCATCACGGTGGAAGACCCCATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAAGCAGCCCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCATGCCGTGGCCTTTGCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTGCTGATGGACCTGTCGCTCAACCT
GCGCGGCATGGTGTCCCAGCGCCTGATCCCCAAGCAGGACGGCAAGGGCCGTGCTGCGGCCGTGGAAATCATGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGATGTCTCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCACTGTTCGACCTGTACGAGGCCAACGTGATCAGCTATGAAGACGCGCTGCGCAACGCCGA
TTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCCAAATCGCCGGACCTTGCCTCGGGCACCGAGC
ACTTCGCGATTGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Legionella pneumophila strain Lp02

44.444

88.095

0.392
  pilT Legionella pneumophila strain ERS1305867

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment