Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAM43_RS23830 Genome accession   NZ_AP028671
Coordinates   5169118..5170161 (+) Length   347 a.a.
NCBI ID   WP_062482334.1    Uniprot ID   A0A554RS02
Organism   Variovorax sp. V77     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5164118..5175161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM43_RS23805 (GmRootV77_47200) - 5164973..5165659 (-) 687 WP_369630303.1 DUF2242 domain-containing protein -
  ACAM43_RS23810 (GmRootV77_47210) - 5165764..5167038 (-) 1275 WP_062478443.1 Glu/Leu/Phe/Val dehydrogenase -
  ACAM43_RS23815 (GmRootV77_47220) - 5167291..5167746 (+) 456 WP_369630304.1 hypothetical protein -
  ACAM43_RS23820 (GmRootV77_47230) - 5167890..5168357 (+) 468 WP_062478450.1 Lrp/AsnC ligand binding domain-containing protein -
  ACAM43_RS23825 (GmRootV77_47240) - 5168365..5169066 (-) 702 WP_369630305.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM43_RS23830 (GmRootV77_47250) pilT 5169118..5170161 (+) 1044 WP_062482334.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM43_RS23835 (GmRootV77_47260) pilU 5170296..5171432 (+) 1137 WP_062482331.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM43_RS23840 (GmRootV77_47270) - 5171499..5172407 (+) 909 WP_369630306.1 NAD(P)-dependent oxidoreductase -
  ACAM43_RS23845 (GmRootV77_47280) - 5172438..5173235 (-) 798 WP_095950095.1 BON domain-containing protein -
  ACAM43_RS23850 (GmRootV77_47290) - 5173232..5173873 (-) 642 WP_062482322.1 SIS domain-containing protein -
  ACAM43_RS23855 (GmRootV77_47300) - 5173959..5174330 (-) 372 WP_139701305.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38132.83 Da        Isoelectric Point: 6.8356

>NTDB_id=106845 ACAM43_RS23830 WP_062482334.1 5169118..5170161(+) (pilT) [Variovorax sp. V77]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=106845 ACAM43_RS23830 WP_062482334.1 5169118..5170161(+) (pilT) [Variovorax sp. V77]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTTCATCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGAACGGCGACGTGCGCCGCATCAACGTCGATGCGCTGGACCACAAGGCCGTGCATGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTTCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTCAACGCGCCGAAGATTTTCGGCGAACTCGCGCTCAAGCCGCGCGGCCTCGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGAATTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCTGTCGTTCGCCAA
CGCATTGCGCTCCGCCCTGCGCGAAGACCCGGACGCCATTCTGGTGGGCGAGTTGCGCGACCTCGAAACCATTCGCCTCG
CGATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAAAAAGAAATGATCCGCGCGATGCTGTCGGAGTCTTTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACGGAGCTGGTGCGCCGCAATACGATCTCTGCCGCCGAAGCGCGCGGCAAGGCCAAGATCCCAGAAAACTTCCCCGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554RS02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment