Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAM43_RS23835 Genome accession   NZ_AP028671
Coordinates   5170296..5171432 (+) Length   378 a.a.
NCBI ID   WP_062482331.1    Uniprot ID   A0A554RS03
Organism   Variovorax sp. V77     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5165296..5176432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM43_RS23810 (GmRootV77_47210) - 5165764..5167038 (-) 1275 WP_062478443.1 Glu/Leu/Phe/Val dehydrogenase -
  ACAM43_RS23815 (GmRootV77_47220) - 5167291..5167746 (+) 456 WP_369630304.1 hypothetical protein -
  ACAM43_RS23820 (GmRootV77_47230) - 5167890..5168357 (+) 468 WP_062478450.1 Lrp/AsnC ligand binding domain-containing protein -
  ACAM43_RS23825 (GmRootV77_47240) - 5168365..5169066 (-) 702 WP_369630305.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM43_RS23830 (GmRootV77_47250) pilT 5169118..5170161 (+) 1044 WP_062482334.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM43_RS23835 (GmRootV77_47260) pilU 5170296..5171432 (+) 1137 WP_062482331.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM43_RS23840 (GmRootV77_47270) - 5171499..5172407 (+) 909 WP_369630306.1 NAD(P)-dependent oxidoreductase -
  ACAM43_RS23845 (GmRootV77_47280) - 5172438..5173235 (-) 798 WP_095950095.1 BON domain-containing protein -
  ACAM43_RS23850 (GmRootV77_47290) - 5173232..5173873 (-) 642 WP_062482322.1 SIS domain-containing protein -
  ACAM43_RS23855 (GmRootV77_47300) - 5173959..5174330 (-) 372 WP_139701305.1 YraN family protein -
  ACAM43_RS23860 (GmRootV77_47310) rsmI 5174377..5175330 (+) 954 WP_369630307.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACAM43_RS23865 (GmRootV77_47320) - 5175333..5176061 (-) 729 WP_369630308.1 glutathione S-transferase -
  ACAM43_RS23870 (GmRootV77_47330) - 5176151..5176378 (-) 228 WP_062481748.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41796.88 Da        Isoelectric Point: 6.3136

>NTDB_id=106846 ACAM43_RS23835 WP_062482331.1 5170296..5171432(+) (pilU) [Variovorax sp. V77]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVAMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGNAINFEEAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=106846 ACAM43_RS23835 WP_062482331.1 5170296..5171432(+) (pilU) [Variovorax sp. V77]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATT
TCGCCGACGGGCATCGGGCGCTTTCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGACTGGGCATGCCGCAGGTGCTCAAGGACGTGGCGATGACCAAGCGCGGCCTCACCA
TCCTCGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTCACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAACGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCCGTGGCCTTCGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGTTCGCTGGTGTCGCAGCGGCTGGTGCCTACCGAAGACGGCCAGGGCCGCGTGGCCGCCGTCGAGATCCTGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCACTGTTCGACCTGTTCGAGGGCAACGCGATCAACTTCGAAGAGGCGATCCGCAACGCAGA
TTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCCGGCACCGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554RS03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Legionella pneumophila strain ERS1305867

44.681

87.037

0.389
  pilT Legionella pneumophila strain Lp02

44.681

87.037

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment