Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACG1BZ_RS01105 Genome accession   NZ_CP172449
Coordinates   231409..232443 (-) Length   344 a.a.
NCBI ID   WP_108731395.1    Uniprot ID   -
Organism   Microbulbifer sp. CNSA002     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 226409..237443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG1BZ_RS01080 (ACG1BZ_01080) - 226687..227844 (+) 1158 WP_413664049.1 sensor histidine kinase -
  ACG1BZ_RS01085 (ACG1BZ_01085) - 227841..228587 (+) 747 WP_413664050.1 LytR/AlgR family response regulator transcription factor -
  ACG1BZ_RS01090 (ACG1BZ_01090) - 228591..229199 (-) 609 WP_413664051.1 LysE family translocator -
  ACG1BZ_RS01095 (ACG1BZ_01095) - 229211..229960 (-) 750 WP_413664052.1 SDR family oxidoreductase -
  ACG1BZ_RS01100 (ACG1BZ_01100) pilU 230197..231348 (-) 1152 WP_108731394.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG1BZ_RS01105 (ACG1BZ_01105) pilT 231409..232443 (-) 1035 WP_108731395.1 type IV pilus twitching motility protein PilT Machinery gene
  ACG1BZ_RS01110 (ACG1BZ_01110) - 232513..233217 (+) 705 WP_413664053.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACG1BZ_RS01115 (ACG1BZ_01115) - 233256..233543 (+) 288 WP_413664054.1 hypothetical protein -
  ACG1BZ_RS01120 (ACG1BZ_01120) - 233624..235639 (-) 2016 WP_413664055.1 prolyl oligopeptidase family serine peptidase -
  ACG1BZ_RS01125 (ACG1BZ_01125) proC 235766..236599 (+) 834 WP_413664056.1 pyrroline-5-carboxylate reductase -
  ACG1BZ_RS01130 (ACG1BZ_01130) - 236667..237275 (+) 609 WP_413664057.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38345.04 Da        Isoelectric Point: 6.5788

>NTDB_id=1067885 ACG1BZ_RS01105 WP_108731395.1 231409..232443(-) (pilT) [Microbulbifer sp. CNSA002]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPSMEHKQVHGLIYEIMNDKQRKDYEELLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVISQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
EDLVARRIISREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1067885 ACG1BZ_RS01105 WP_108731395.1 231409..232443(-) (pilT) [Microbulbifer sp. CNSA002]
ATGGATATCACTGAACTCCTCGCCTTTAGCGCCAAGCAAAACGCCTCGGACTTACACCTCTCGGCGGGCCTGCCGCCGAT
GATCCGTGTCGACGGCGACGTGCGCCGTATCAACCTGCCCTCAATGGAGCACAAGCAGGTACACGGCCTGATCTACGAGA
TCATGAACGATAAGCAGCGCAAGGATTACGAAGAACTACTGGAAACAGACTTCTCTTTTGAAGTCCCCGGTGTCGCCCGC
TTTCGTGTAAACGCCTTTAACCACAACCGAGGTGCCGGCGCCGTATTCCGGACCATTCCCTCCAAGGTTCTCACTATGGA
AGACCTGGGAATGGGTCAGGTGTTTAAACAGATCTCAGATACTCCCCGTGGCTTGGTACTGGTGACCGGGCCCACGGGTT
CCGGTAAGTCCACCACCCTGGCGGCGATGATGGACTACATCAACGACAATAAATACGAGCATATCCTCACCATCGAGGAC
CCGATCGAATTTGTGCACGAATCCAAGAAGTGCCTGGTGAACCAGCGTGAGGTGCACCGGGATACCCACGGCTTTGCCGA
AGCGCTGCGCTCTGCACTGCGTGAAGACCCGGATATCATTCTCGTGGGTGAGATGCGGGACCTGGAGACCATTCGCCTGG
CGCTGACTGCGGCAGAGACCGGTCACTTGGTATTCGGCACCCTGCACACCACTTCCGCCGCCAAAACCATTGACCGTGTG
GTAGATGTATTCCCGGCGCAGGAGAAAGCCATGGTGCGTTCAATGCTGTCGGAATCCCTGCAGGCCGTTATCTCCCAGAC
GCTAATGAAGAAAAACGGCGGTGGCCGGGTCGCTGCCCACGAAATTATGCGCGGTACCCCAGCCATCCGTAACCTGATCC
GCGAAGACAAAATCGCGCAGATGTACTCGGCCATCCAGACCGGAGCCAGCGTCGGCATGCAGACCATGGACCAATGCCTG
GAAGACCTGGTGGCACGCCGCATCATCAGCCGCGAAGTGGCTCGCGAAAAAGCCAAGATGCCGGAGAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.244

100

0.892
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.547

0.404
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment