Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACG1BZ_RS01100 Genome accession   NZ_CP172449
Coordinates   230197..231348 (-) Length   383 a.a.
NCBI ID   WP_108731394.1    Uniprot ID   -
Organism   Microbulbifer sp. CNSA002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 225197..236348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG1BZ_RS01080 (ACG1BZ_01080) - 226687..227844 (+) 1158 WP_413664049.1 sensor histidine kinase -
  ACG1BZ_RS01085 (ACG1BZ_01085) - 227841..228587 (+) 747 WP_413664050.1 LytR/AlgR family response regulator transcription factor -
  ACG1BZ_RS01090 (ACG1BZ_01090) - 228591..229199 (-) 609 WP_413664051.1 LysE family translocator -
  ACG1BZ_RS01095 (ACG1BZ_01095) - 229211..229960 (-) 750 WP_413664052.1 SDR family oxidoreductase -
  ACG1BZ_RS01100 (ACG1BZ_01100) pilU 230197..231348 (-) 1152 WP_108731394.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG1BZ_RS01105 (ACG1BZ_01105) pilT 231409..232443 (-) 1035 WP_108731395.1 type IV pilus twitching motility protein PilT Machinery gene
  ACG1BZ_RS01110 (ACG1BZ_01110) - 232513..233217 (+) 705 WP_413664053.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACG1BZ_RS01115 (ACG1BZ_01115) - 233256..233543 (+) 288 WP_413664054.1 hypothetical protein -
  ACG1BZ_RS01120 (ACG1BZ_01120) - 233624..235639 (-) 2016 WP_413664055.1 prolyl oligopeptidase family serine peptidase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42498.72 Da        Isoelectric Point: 5.9763

>NTDB_id=1067884 ACG1BZ_RS01100 WP_108731394.1 230197..231348(-) (pilU) [Microbulbifer sp. CNSA002]
MDIERLLQLVVEKGASDLFITAGVPPSIKLHGRVVPCSSTALTPEKARELVVGTMNEKQRREFAEQKELNFAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPDVLKDLAMTKRGLIIFVGATGTGKSTSLASMIGHRNENTKGHIITIED
PIEFIHQHRGCIVTQREVGLDTESFDVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHKQLFMDLSLNLKAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLIRKGEVDKIKELMKRSGEQGMQTFDQA
LYELYDGGEITYEDALSHADSANDLRLMIKLKSESDAEYLNSAAGELSLQNDSEYGDGGPQLY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=1067884 ACG1BZ_RS01100 WP_108731394.1 230197..231348(-) (pilU) [Microbulbifer sp. CNSA002]
ATGGATATTGAAAGACTTCTACAACTGGTTGTAGAGAAGGGCGCTTCGGATTTATTTATCACGGCGGGCGTCCCGCCCTC
CATCAAACTGCACGGCCGAGTTGTGCCCTGTAGCAGCACCGCGCTCACGCCAGAGAAAGCGCGCGAGCTGGTGGTGGGAA
CCATGAACGAGAAACAGCGGCGCGAGTTTGCCGAGCAAAAGGAATTGAACTTCGCGATTGCAGTGCGCAACGTGGGGCGT
TTCCGGGTATCGGCCTTCTTCCAGCGCAATCTGGTGGGTATGGTGCTGCGTCGCATCGAAACCAAAATCCCCACTATCGA
TGGACTCGGACTACCCGACGTACTCAAAGACCTGGCCATGACCAAACGCGGGCTGATCATTTTTGTGGGCGCCACCGGTA
CCGGTAAGTCCACATCCCTCGCCTCCATGATCGGCCACCGCAATGAGAACACCAAGGGCCATATCATCACCATTGAGGAC
CCGATCGAATTTATCCACCAGCATCGCGGCTGTATTGTGACCCAGCGCGAAGTGGGCCTGGATACCGAGTCTTTCGATGT
AGCCCTGAAAAACACTCTGCGTCAGGCACCAGATGTTATTCTGATCGGTGAGGTGCGCTCCCGCGAAACCATGGACCACG
CCATCGCTTTCGCCGAGACCGGCCATTTGTGTCTGTGTACCCTCCACGCCAACAATGCTAACCAGGCCCTCGATCGGATC
ATTCACTTCTTCCCGGCAGACCGCCACAAACAGTTGTTTATGGACCTGTCCCTCAATCTGAAGGCGATGGTGGCCCAGCA
GCTGGTACCGACCCCCGATGGCGATGGCCGCCGTGCCTGCCTTGAGATCATGATCAATACCCCGCTGATGTCGGACCTGA
TCCGCAAAGGCGAAGTGGATAAGATCAAGGAGTTGATGAAGCGCTCTGGTGAGCAGGGAATGCAGACCTTCGACCAGGCA
CTCTACGAGCTCTACGATGGGGGAGAAATTACCTATGAGGATGCCCTCTCCCACGCCGACTCCGCCAACGATTTGCGCCT
GATGATTAAGCTCAAATCCGAATCCGATGCCGAGTACTTAAATAGTGCCGCTGGCGAGTTGAGTCTACAAAATGATTCAG
AGTACGGAGACGGTGGTCCTCAGCTCTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.135

96.606

0.726
  pilU Acinetobacter baylyi ADP1

67.787

93.211

0.632
  pilU Vibrio cholerae strain A1552

54.987

96.867

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.313

88.512

0.41
  pilT Acinetobacter nosocomialis M2

43.068

88.512

0.381
  pilT Acinetobacter baumannii D1279779

43.068

88.512

0.381
  pilT Acinetobacter baumannii strain A118

43.068

88.512

0.381
  pilT Pseudomonas aeruginosa PAK

41.888

88.512

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.84

87.99

0.368
  pilT Vibrio cholerae strain A1552

41.84

87.99

0.368
  pilT Acinetobacter baylyi ADP1

41.056

89.034

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.298

88.512

0.366
  pilT Legionella pneumophila strain Lp02

41.791

87.467

0.366
  pilT Legionella pneumophila strain ERS1305867

41.791

87.467

0.366

Multiple sequence alignment