Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAM55_RS01930 Genome accession   NZ_AP028664
Coordinates   375170..376213 (-) Length   347 a.a.
NCBI ID   WP_369654423.1    Uniprot ID   -
Organism   Variovorax sp. V213     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 370170..381213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM55_RS01905 (GmRootV213_03780) - 371081..371457 (+) 377 Protein_378 YraN family protein -
  ACAM55_RS01910 (GmRootV213_03790) - 371523..372167 (+) 645 WP_369654419.1 SIS domain-containing protein -
  ACAM55_RS01915 (GmRootV213_03800) - 372164..372916 (+) 753 WP_369654420.1 BON domain-containing protein -
  ACAM55_RS01920 (GmRootV213_03810) - 372959..373867 (-) 909 WP_369654421.1 NAD(P)-dependent oxidoreductase -
  ACAM55_RS01925 (GmRootV213_03820) pilU 374005..375141 (-) 1137 WP_369654422.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM55_RS01930 (GmRootV213_03830) pilT 375170..376213 (-) 1044 WP_369654423.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM55_RS01935 (GmRootV213_03840) - 376265..376966 (+) 702 WP_369654424.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM55_RS01940 (GmRootV213_03850) - 376969..377442 (-) 474 WP_369654425.1 Lrp/AsnC ligand binding domain-containing protein -
  ACAM55_RS01945 (GmRootV213_03860) - 377531..380503 (+) 2973 WP_369654426.1 L-glutamate gamma-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38095.82 Da        Isoelectric Point: 6.8885

>NTDB_id=106767 ACAM55_RS01930 WP_369654423.1 375170..376213(-) (pilT) [Variovorax sp. V213]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSAIQTGQGAGMQTLDQN
LTDLVRRNAISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=106767 ACAM55_RS01930 WP_369654423.1 375170..376213(-) (pilT) [Variovorax sp. V213]
GTGGACATTACCCAACTGCTGGCATTCAGCGTCAAGAACAAAGCCTCCGACCTGCATTTGTCGGCCGGGCTCCCGCCGAT
GATTCGCGTTCACGGCGACGTGCGGCGCATCAACGTCGATGCGCTCGACCACAAGGCGGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTGGCGCGC
TTCCGCGTGAATGCATTCAACCAGGCGCGCGGCGCGGCCGCGGTGTTCCGGACCATTCCTTCGAAGATCCTCACGCTGGA
GCAGCTGAACGCGCCGAAGATTTTCGCGGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGGCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGGTCAACTACCTGAACGAAAACGAGTACGGCCACATCCTGACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTCTCGTTCTCCAA
CGCCCTGCGCTCGGCACTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAGTTGCGCGACCTGGAAACCATTCGCCTCG
CGATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAAAAGGAAATGATCCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGGGCGCGGGCATGCAGACACTGGACCAGAAC
CTGACCGACCTCGTCCGGCGCAATGCCATTTCGGCGGCCGAGGCGCGCGGCAAGGCCAAGATCCCCGAAAATTTCCCAGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.924

95.389

0.715
  pilT Legionella pneumophila strain ERS1305867

74.924

95.389

0.715
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment