Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAM55_RS01925 Genome accession   NZ_AP028664
Coordinates   374005..375141 (-) Length   378 a.a.
NCBI ID   WP_369654422.1    Uniprot ID   -
Organism   Variovorax sp. V213     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 369005..380141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM55_RS01895 (GmRootV213_03760) - 369340..370071 (+) 732 WP_369654417.1 glutathione S-transferase -
  ACAM55_RS01900 (GmRootV213_03770) rsmI 370074..371033 (-) 960 WP_369654418.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACAM55_RS01905 (GmRootV213_03780) - 371081..371457 (+) 377 Protein_378 YraN family protein -
  ACAM55_RS01910 (GmRootV213_03790) - 371523..372167 (+) 645 WP_369654419.1 SIS domain-containing protein -
  ACAM55_RS01915 (GmRootV213_03800) - 372164..372916 (+) 753 WP_369654420.1 BON domain-containing protein -
  ACAM55_RS01920 (GmRootV213_03810) - 372959..373867 (-) 909 WP_369654421.1 NAD(P)-dependent oxidoreductase -
  ACAM55_RS01925 (GmRootV213_03820) pilU 374005..375141 (-) 1137 WP_369654422.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM55_RS01930 (GmRootV213_03830) pilT 375170..376213 (-) 1044 WP_369654423.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM55_RS01935 (GmRootV213_03840) - 376265..376966 (+) 702 WP_369654424.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM55_RS01940 (GmRootV213_03850) - 376969..377442 (-) 474 WP_369654425.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41873.93 Da        Isoelectric Point: 6.4189

>NTDB_id=106766 ACAM55_RS01925 WP_369654422.1 374005..375141(-) (pilU) [Variovorax sp. V213]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLTIMVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=106766 ACAM55_RS01925 WP_369654422.1 374005..375141(-) (pilU) [Variovorax sp. V213]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
CACCGCCGACTTTCCGCCCGCGATCAAGGTGGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAATGCAACTTTGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
GGCCAAGCTGCCGACCATCGACGGGCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCCATGACCAAGCGCGGCCTCACCA
TCATGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAATTCCTACGGC
CACATCGTGACGGTGGAAGACCCGGTCGAGTTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACGGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATTTTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCATGCAGTGGCTTTTGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTCTCGCAGCGCCTGGTGCCCACCGAGGACGGGCTGGGCCGCGTGGCCGCGGTGGAAGTGCTGCTGAACA
CGCCGCTCATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGCGCAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACCTGTTCGAGAGCCATTCGATCACTTTTGAAGACGCCATCCGCAACGCCGA
CTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCACGCAGCACCGACCTCTCGGCAGGCACCGAGC
ATTTCGCGATTGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

57.263

94.709

0.542
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Legionella pneumophila strain ERS1305867

43.465

87.037

0.378
  pilT Legionella pneumophila strain Lp02

43.465

87.037

0.378
  pilT Acinetobacter baumannii D1279779

44.514

84.392

0.376
  pilT Acinetobacter baumannii strain A118

44.514

84.392

0.376
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373

Multiple sequence alignment