Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACHFB9_RS01150 Genome accession   NZ_CP172404
Coordinates   260229..261272 (+) Length   347 a.a.
NCBI ID   WP_417069144.1    Uniprot ID   -
Organism   Niveibacterium terrae strain 24SJ04S-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 255229..266272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFB9_RS01125 (ACHFB9_01125) - 255688..257841 (+) 2154 WP_417069139.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  ACHFB9_RS01130 (ACHFB9_01130) pdxH 257846..258436 (-) 591 WP_417069140.1 pyridoxamine 5'-phosphate oxidase -
  ACHFB9_RS01135 (ACHFB9_01135) - 258498..258806 (-) 309 WP_417069141.1 DUF167 domain-containing protein -
  ACHFB9_RS01140 (ACHFB9_01140) - 258806..259360 (-) 555 WP_417069142.1 YggT family protein -
  ACHFB9_RS01145 (ACHFB9_01145) - 259447..260145 (-) 699 WP_417069143.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHFB9_RS01150 (ACHFB9_01150) pilT 260229..261272 (+) 1044 WP_417069144.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHFB9_RS01155 (ACHFB9_01155) pilU 261326..262459 (+) 1134 WP_417069145.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHFB9_RS01160 (ACHFB9_01160) - 262546..263229 (+) 684 WP_417069146.1 head GIN domain-containing protein -
  ACHFB9_RS01165 (ACHFB9_01165) thrH 263307..263915 (+) 609 WP_417069147.1 bifunctional phosphoserine phosphatase/homoserine phosphotransferase ThrH -
  ACHFB9_RS01170 (ACHFB9_01170) purU 263947..264807 (+) 861 WP_417069148.1 formyltetrahydrofolate deformylase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38803.53 Da        Isoelectric Point: 6.6617

>NTDB_id=1067406 ACHFB9_RS01150 WP_417069144.1 260229..261272(+) (pilT) [Niveibacterium terrae strain 24SJ04S-2]
MDITELLAFAVKNKASDLHLSSGLPPMIRVHGDIRRINLPPMEHKEVHAMVYDIMNDSQRKTFEENWECDFSFNVPNLAR
FRVNAFTQDRGAAAVFRTIPSKVLTLEELECPKIFKDIAQNPRGIVLVTGPTGSGKSTTLAAMVDYLNENEYGHILTIED
PIEFVHESKRCLINQREVSRDTHSFNNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLRAVISQTLLKKKDASGRVAAHEIMIGTPAIRNLIREAKIAQMYSSIQTGQNFGMQTLDQC
LQELVKRNIVGVAEARLRAANKDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1067406 ACHFB9_RS01150 WP_417069144.1 260229..261272(+) (pilT) [Niveibacterium terrae strain 24SJ04S-2]
ATGGACATCACCGAGCTGCTCGCCTTTGCGGTCAAGAACAAGGCTTCCGACCTTCACCTCTCCTCCGGTTTGCCGCCGAT
GATTCGCGTCCACGGCGACATCCGGCGGATCAATCTTCCGCCGATGGAGCACAAGGAAGTCCACGCCATGGTGTACGACA
TCATGAACGACTCCCAGCGCAAGACTTTCGAAGAGAACTGGGAGTGCGACTTCTCGTTCAACGTGCCCAACCTCGCGCGT
TTCCGGGTCAATGCTTTCACCCAGGACCGGGGCGCGGCGGCGGTGTTCCGGACCATTCCGTCCAAGGTGCTGACCCTCGA
AGAGCTCGAATGCCCGAAGATCTTCAAGGACATCGCACAGAACCCGCGCGGCATCGTGCTGGTCACCGGACCGACCGGTT
CGGGCAAGTCGACGACGCTGGCGGCGATGGTCGACTACCTCAACGAGAACGAATACGGCCACATCCTGACGATCGAGGAT
CCGATCGAATTCGTCCATGAGTCCAAGCGCTGCCTGATCAACCAGCGCGAAGTCTCGCGCGACACCCACTCCTTCAACAA
CGCGCTGCGCTCGGCGCTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTTGAAACGATTCGTCTGG
CGCTGACTGCTGCGGAAACGGGCCACCTGGTGTTCGGTACCCTGCACACGTCGTCGGCCGCGAAAACGATCGACCGTATC
ATCGACGTGTTTCCTGCCGAGGAAAAGGAAATGGTCCGCGCGATGCTGTCCGAATCGCTGCGGGCGGTGATCTCGCAGAC
TCTGCTGAAGAAGAAGGACGCTTCGGGCCGGGTCGCCGCGCACGAGATCATGATCGGCACCCCGGCGATCCGGAACCTGA
TCCGCGAAGCGAAGATCGCGCAGATGTACTCGTCGATCCAGACCGGGCAGAACTTCGGCATGCAGACGCTGGACCAGTGC
CTGCAGGAACTCGTCAAGCGCAATATCGTTGGCGTCGCCGAAGCGCGCTTGCGCGCGGCCAACAAGGACAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.681

99.424

0.772
  pilT Acinetobacter nosocomialis M2

77.681

99.424

0.772
  pilT Acinetobacter baumannii strain A118

77.681

99.424

0.772
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Legionella pneumophila strain Lp02

73.623

99.424

0.732
  pilT Legionella pneumophila strain ERS1305867

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

70.145

99.424

0.697
  pilT Neisseria gonorrhoeae MS11

69.855

99.424

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.857

96.83

0.657
  pilT Vibrio cholerae strain A1552

67.857

96.83

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Pseudomonas stutzeri DSM 10701

41.159

99.424

0.409
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Vibrio cholerae strain A1552

42.462

93.66

0.398

Multiple sequence alignment