Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHFB9_RS01155 Genome accession   NZ_CP172404
Coordinates   261326..262459 (+) Length   377 a.a.
NCBI ID   WP_417069145.1    Uniprot ID   -
Organism   Niveibacterium terrae strain 24SJ04S-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 256326..267459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFB9_RS01130 (ACHFB9_01130) pdxH 257846..258436 (-) 591 WP_417069140.1 pyridoxamine 5'-phosphate oxidase -
  ACHFB9_RS01135 (ACHFB9_01135) - 258498..258806 (-) 309 WP_417069141.1 DUF167 domain-containing protein -
  ACHFB9_RS01140 (ACHFB9_01140) - 258806..259360 (-) 555 WP_417069142.1 YggT family protein -
  ACHFB9_RS01145 (ACHFB9_01145) - 259447..260145 (-) 699 WP_417069143.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHFB9_RS01150 (ACHFB9_01150) pilT 260229..261272 (+) 1044 WP_417069144.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHFB9_RS01155 (ACHFB9_01155) pilU 261326..262459 (+) 1134 WP_417069145.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHFB9_RS01160 (ACHFB9_01160) - 262546..263229 (+) 684 WP_417069146.1 head GIN domain-containing protein -
  ACHFB9_RS01165 (ACHFB9_01165) thrH 263307..263915 (+) 609 WP_417069147.1 bifunctional phosphoserine phosphatase/homoserine phosphotransferase ThrH -
  ACHFB9_RS01170 (ACHFB9_01170) purU 263947..264807 (+) 861 WP_417069148.1 formyltetrahydrofolate deformylase -
  ACHFB9_RS01175 (ACHFB9_01175) - 264836..266935 (-) 2100 WP_417069149.1 TonB-dependent receptor -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42234.52 Da        Isoelectric Point: 6.3141

>NTDB_id=1067407 ACHFB9_RS01155 WP_417069145.1 261326..262459(+) (pilU) [Niveibacterium terrae strain 24SJ04S-2]
MERDQALKFMHDLLRLMISKRGSDLFITAGFPPAIKVDGKIVPQSNQVLTAQHTAELVRSVMNDRQAAEFESTKECNFAI
SPAGIGRFRTNAFVQQSRVGMVCRTIAQKIPTLDELGMPQVLKDIAMSKRGLVIFVGGTGTGKTTSLAGLVDYRNENSFG
HIITLEDPIEYVHAHKNCVVTQREVGIDTDSWEAGLKNTLRQAPDVILMGEIRDRETMDYAVAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRGMVSQRLLPLKDRKGRVPAVEVLLNSPLIADLIFKGEIAEIKDLMKRSRELG
MQTFDQSLFELYENELISYEDALRNADSINDLRLQIKLNSRHSDRDLAAGIQNLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1067407 ACHFB9_RS01155 WP_417069145.1 261326..262459(+) (pilU) [Niveibacterium terrae strain 24SJ04S-2]
ATGGAACGCGATCAGGCACTCAAATTCATGCACGACCTGCTGCGGCTGATGATCAGCAAGCGCGGCTCCGACCTTTTCAT
CACCGCGGGTTTCCCGCCGGCGATCAAGGTCGACGGCAAGATCGTGCCCCAGTCCAATCAGGTCCTGACCGCGCAGCACA
CGGCCGAACTGGTTCGCTCGGTCATGAACGACCGTCAGGCGGCCGAGTTCGAATCGACTAAGGAGTGCAACTTCGCGATC
TCCCCGGCCGGGATCGGGCGCTTCCGGACCAATGCCTTTGTTCAGCAGAGTCGGGTCGGCATGGTCTGCCGGACGATCGC
GCAGAAGATTCCGACGCTCGACGAGTTGGGCATGCCCCAGGTGCTCAAGGATATCGCGATGTCCAAGCGCGGCCTCGTGA
TCTTCGTCGGCGGCACTGGCACCGGCAAGACGACTTCGCTCGCCGGCCTCGTCGACTACCGAAACGAGAACAGCTTCGGC
CACATCATTACGCTCGAAGATCCGATCGAATACGTCCATGCGCACAAGAACTGCGTCGTGACCCAGCGCGAAGTCGGTAT
CGATACCGATAGCTGGGAAGCGGGACTGAAGAACACGCTGCGTCAGGCGCCTGACGTGATCCTGATGGGCGAAATCCGCG
ACCGGGAAACGATGGACTATGCGGTTGCGTTTGCCGAGACCGGCCACCTCTGTCTGGCGACCTTGCACGCGAACAGCGCC
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCCGAAGACCGCCGCCAGCAGCTTCTGATGGACTTGTCGCTGAACCT
GCGCGGCATGGTTTCGCAGCGCCTGTTGCCATTGAAGGATCGTAAGGGGCGGGTGCCGGCGGTTGAAGTACTGCTCAATT
CTCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGATCGCCGAGATCAAGGATTTGATGAAGCGCTCGCGCGAACTGGGT
ATGCAGACTTTCGACCAGTCGCTGTTCGAATTGTACGAGAACGAGCTGATCAGCTACGAGGACGCGCTGCGCAATGCCGA
TTCGATCAACGACCTGCGCCTGCAGATCAAACTGAACAGCCGGCACTCGGATCGCGACCTGGCGGCGGGTATCCAGAATC
TCGATATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.462

96.552

0.613
  pilU Acinetobacter baylyi ADP1

59.885

92.573

0.554
  pilU Vibrio cholerae strain A1552

53.611

95.491

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.478

88.859

0.395
  pilT Legionella pneumophila strain Lp02

42.296

87.798

0.371
  pilT Legionella pneumophila strain ERS1305867

42.296

87.798

0.371
  pilT Pseudomonas aeruginosa PAK

41.493

88.859

0.369
  pilT Acinetobacter nosocomialis M2

41.39

87.798

0.363
  pilT Acinetobacter baumannii D1279779

41.39

87.798

0.363
  pilT Acinetobacter baumannii strain A118

41.39

87.798

0.363
  pilT Pseudomonas stutzeri DSM 10701

40.597

88.859

0.361
  pilT Acinetobacter baylyi ADP1

40.356

89.39

0.361

Multiple sequence alignment