Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAM57_RS02730 Genome accession   NZ_AP028659
Coordinates   547341..548384 (-) Length   347 a.a.
NCBI ID   WP_093206426.1    Uniprot ID   A0A7Y7IBT6
Organism   Variovorax sp. V15     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542341..553384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM57_RS02705 (GmRootV15_05300) - 543283..543660 (+) 378 WP_369660623.1 YraN family protein -
  ACAM57_RS02710 (GmRootV15_05310) - 543732..544343 (+) 612 WP_126025779.1 SIS domain-containing protein -
  ACAM57_RS02715 (GmRootV15_05320) - 544340..545164 (+) 825 WP_369660624.1 BON domain-containing protein -
  ACAM57_RS02720 (GmRootV15_05330) - 545219..546127 (-) 909 WP_369660625.1 NAD(P)-dependent oxidoreductase -
  ACAM57_RS02725 (GmRootV15_05340) pilU 546176..547312 (-) 1137 WP_369660626.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM57_RS02730 (GmRootV15_05350) pilT 547341..548384 (-) 1044 WP_093206426.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM57_RS02735 (GmRootV15_05360) - 548436..549140 (+) 705 WP_369660627.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM57_RS02740 (GmRootV15_05370) - 549151..549618 (-) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ACAM57_RS02745 (GmRootV15_05380) - 549762..550220 (-) 459 WP_184642078.1 hypothetical protein -
  ACAM57_RS02750 (GmRootV15_05390) - 550347..550670 (-) 324 WP_369660628.1 DUF4148 domain-containing protein -
  ACAM57_RS02755 (GmRootV15_05400) - 550904..552178 (+) 1275 WP_184642076.1 Glu/Leu/Phe/Val dehydrogenase -
  ACAM57_RS02760 (GmRootV15_05410) - 552213..552734 (+) 522 WP_126025787.1 alpha-ketoglutarate-dependent dioxygenase AlkB -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38148.83 Da        Isoelectric Point: 6.8356

>NTDB_id=106717 ACAM57_RS02730 WP_093206426.1 547341..548384(-) (pilT) [Variovorax sp. V15]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=106717 ACAM57_RS02730 WP_093206426.1 547341..548384(-) (pilT) [Variovorax sp. V15]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTTTCCGCGGGCCTGCCGCCCAT
GATCCGCGTCAACGGCGACGTGCGCCGCATCAACGTCGACGCGCTCGACCACAAGGGCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCCTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCACGCGGCGCGGCCGCGGTGTTCCGGACCATTCCGTCCAAGATCCTCACGCTCGA
GCAGCTGAACGCGCCGAAGATTTTCGGTGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGGGGCCGATGACGCTGTCGTTCTCCAA
CGCCCTGCGCTCCGCGCTGCGCGAAGACCCCGACGCCATCCTGGTGGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CGATGACCGCCGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCCTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAGAAGGAAATGATCCGCGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACAAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTGGACCAGAAC
CTGACGGAACTGGTGCGCCGCAATACCATCTCGGCAGCGGAAGCCCGCGGGAAAGCCAAGATCCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y7IBT6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.336

97.695

0.726
  pilT Legionella pneumophila strain ERS1305867

74.336

97.695

0.726
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.622

98.271

0.674
  pilT Vibrio cholerae strain A1552

68.622

98.271

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment