Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAM57_RS02725 Genome accession   NZ_AP028659
Coordinates   546176..547312 (-) Length   378 a.a.
NCBI ID   WP_369660626.1    Uniprot ID   -
Organism   Variovorax sp. V15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541176..552312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM57_RS02695 (GmRootV15_05280) - 541538..542266 (+) 729 WP_184642083.1 glutathione S-transferase -
  ACAM57_RS02700 (GmRootV15_05290) rsmI 542263..543213 (-) 951 WP_369660622.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACAM57_RS02705 (GmRootV15_05300) - 543283..543660 (+) 378 WP_369660623.1 YraN family protein -
  ACAM57_RS02710 (GmRootV15_05310) - 543732..544343 (+) 612 WP_126025779.1 SIS domain-containing protein -
  ACAM57_RS02715 (GmRootV15_05320) - 544340..545164 (+) 825 WP_369660624.1 BON domain-containing protein -
  ACAM57_RS02720 (GmRootV15_05330) - 545219..546127 (-) 909 WP_369660625.1 NAD(P)-dependent oxidoreductase -
  ACAM57_RS02725 (GmRootV15_05340) pilU 546176..547312 (-) 1137 WP_369660626.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM57_RS02730 (GmRootV15_05350) pilT 547341..548384 (-) 1044 WP_093206426.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM57_RS02735 (GmRootV15_05360) - 548436..549140 (+) 705 WP_369660627.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM57_RS02740 (GmRootV15_05370) - 549151..549618 (-) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ACAM57_RS02745 (GmRootV15_05380) - 549762..550220 (-) 459 WP_184642078.1 hypothetical protein -
  ACAM57_RS02750 (GmRootV15_05390) - 550347..550670 (-) 324 WP_369660628.1 DUF4148 domain-containing protein -
  ACAM57_RS02755 (GmRootV15_05400) - 550904..552178 (+) 1275 WP_184642076.1 Glu/Leu/Phe/Val dehydrogenase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41824.95 Da        Isoelectric Point: 6.4175

>NTDB_id=106716 ACAM57_RS02725 WP_369660626.1 546176..547312(-) (pilU) [Variovorax sp. V15]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAPHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLCILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLVPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSISFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=106716 ACAM57_RS02725 WP_369660626.1 546176..547312(-) (pilU) [Variovorax sp. V15]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTTTCGCAGCAGGCGCTGGGCGCGCCGCACA
CGCTGGCGCTGACCCGCTCGATCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAATGCAATTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTGACGGTGGAAGACCCGGTCGAATTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTCATTCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCCGTGGCTTTCGCCGAAACGGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAACGCCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGATTTCGCAACGGCTGGTGCCAACGGAAGACGGCCTGGGCCGCGTGGCCGCGGTGGAAGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAAATCATGCGCAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTTCGAGAGCCACTCGATCAGCTTCGAGGACGCCATCCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCGGGCACGGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Pseudomonas aeruginosa PAK

45.697

89.153

0.407
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Legionella pneumophila strain Lp02

43.769

87.037

0.381
  pilT Legionella pneumophila strain ERS1305867

43.769

87.037

0.381
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362

Multiple sequence alignment