Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACH3PB_RS16355 Genome accession   NZ_CP172312
Coordinates   3645678..3646712 (-) Length   344 a.a.
NCBI ID   WP_416396507.1    Uniprot ID   -
Organism   Allohahella sp. A8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3640678..3651712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH3PB_RS16345 (ACH3PB_16345) - 3642774..3644402 (+) 1629 WP_416396505.1 methyl-accepting chemotaxis protein -
  ACH3PB_RS16350 (ACH3PB_16350) pilU 3644481..3645617 (-) 1137 WP_416396506.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACH3PB_RS16355 (ACH3PB_16355) pilT 3645678..3646712 (-) 1035 WP_416396507.1 type IV pilus twitching motility protein PilT Machinery gene
  ACH3PB_RS16360 (ACH3PB_16360) - 3646802..3647551 (+) 750 WP_416396508.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACH3PB_RS16365 (ACH3PB_16365) proC 3647565..3648428 (+) 864 WP_416396509.1 pyrroline-5-carboxylate reductase -
  ACH3PB_RS16370 (ACH3PB_16370) - 3648510..3649076 (+) 567 WP_416396510.1 YggT family protein -
  ACH3PB_RS16375 (ACH3PB_16375) - 3649219..3651216 (+) 1998 WP_416396511.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37964.62 Da        Isoelectric Point: 6.4180

>NTDB_id=1066257 ACH3PB_RS16355 WP_416396507.1 3645678..3646712(-) (pilT) [Allohahella sp. A8]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALEHKEVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGLGQVFKDIASKPRGLCLVTGPTGSGKSTTLAAMIDYVNDNRYDHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAAEKSMVRSMLSESLQAVISQTLMKKVGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGNIGMQTLDQCM
QGLLSKGLITRDQAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1066257 ACH3PB_RS16355 WP_416396507.1 3645678..3646712(-) (pilT) [Allohahella sp. A8]
ATGGATATTACTGAATTGCTCGCGTTCTCCGCCAAACAGGGAGCCTCGGACCTCCACCTGTCTGCCGGACTGCCGCCGAT
GATTCGTGTCGATGGTGACGTACGACGCATCAACCTGCCAGCCCTCGAACATAAGGAAGTTCACGCGCTCATTTATGACA
TCATGAATGACAAACAGCGCAAGGACTTCGAAGAATTCCTCGAAACCGACTTCTCCTTCGAAGTGCCCGGCGTCGCGCGA
TTCCGTGTCAACGCCTTCAACCAGAACCGTGGTGCAGGTGCGGTGTTCCGGACCATTCCTTCGAAGGTCCTGAGTATGGA
CGACCTCGGGCTTGGTCAGGTTTTCAAAGACATCGCCAGTAAACCGCGCGGCCTGTGTCTGGTCACTGGGCCAACCGGTT
CGGGCAAGAGTACGACCCTTGCGGCGATGATCGACTACGTCAACGACAATCGCTACGACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTTCACGAGAGCAAGAAATGCCTGGTGAACCAGCGCGAAGTGCACCGTGACACCCTGGGCTTCAGCGA
GGCCCTGCGTTCAGCGCTGCGGGAAGACCCTGACATCATCCTCGTCGGCGAGATGCGTGACCTTGAAACCATCCGTCTCG
CGCTGACTGCGGCGGAAACGGGTCACCTCGTGTTCGGCACACTGCACACCACCTCTGCGGCCAAGACCATTGACCGCGTG
GTCGACGTGTTTCCCGCCGCAGAAAAATCAATGGTTCGATCGATGCTGTCTGAATCACTGCAGGCGGTCATTTCGCAGAC
GCTCATGAAGAAGGTTGGCGGTGGTCGTATCGCCGCCCACGAAATCATGATCGGCACCCCGGCGATTCGAAACCTGATCC
GTGAGGACAAGGTCGCACAGATGTATTCGTCTATTCAGACCGGCGGCAACATCGGCATGCAGACGCTGGATCAATGCATG
CAGGGCTTGCTGTCCAAGGGTCTTATCACTCGTGATCAGGCGCGTGAGAAAGCCAAGATTCCCGAGAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

90.407

100

0.904
  pilT Pseudomonas aeruginosa PAK

89.535

100

0.895
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.395

100

0.814
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.765

98.837

0.66
  pilT Vibrio cholerae strain A1552

66.765

98.837

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395

Multiple sequence alignment