Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACH3PB_RS16350 Genome accession   NZ_CP172312
Coordinates   3644481..3645617 (-) Length   378 a.a.
NCBI ID   WP_416396506.1    Uniprot ID   -
Organism   Allohahella sp. A8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3639481..3650617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH3PB_RS16340 (ACH3PB_16340) polA 3639873..3642674 (+) 2802 WP_416396504.1 DNA polymerase I -
  ACH3PB_RS16345 (ACH3PB_16345) - 3642774..3644402 (+) 1629 WP_416396505.1 methyl-accepting chemotaxis protein -
  ACH3PB_RS16350 (ACH3PB_16350) pilU 3644481..3645617 (-) 1137 WP_416396506.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACH3PB_RS16355 (ACH3PB_16355) pilT 3645678..3646712 (-) 1035 WP_416396507.1 type IV pilus twitching motility protein PilT Machinery gene
  ACH3PB_RS16360 (ACH3PB_16360) - 3646802..3647551 (+) 750 WP_416396508.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACH3PB_RS16365 (ACH3PB_16365) proC 3647565..3648428 (+) 864 WP_416396509.1 pyrroline-5-carboxylate reductase -
  ACH3PB_RS16370 (ACH3PB_16370) - 3648510..3649076 (+) 567 WP_416396510.1 YggT family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41733.95 Da        Isoelectric Point: 6.6359

>NTDB_id=1066256 ACH3PB_RS16350 WP_416396506.1 3644481..3645617(-) (pilU) [Allohahella sp. A8]
MDFNKLLKVMVEKGASDLFITAGVPPSMKVNGRMLPITKTSLSPEETREIVFSIMSDKQQSDFEAHHECNFAISARGIGR
FRVSAFYQRNLCGMVLRRIETNIPSVDDLRLPETIKELAMAKRGLIIFVGATGTGKSTSLAAMIGHRNANSKGHIISIED
PIEFIHQHRGCIVTQREVGLDTESFEMALKNTLRQAPDVIMIGEVRSPDTMEHAVVFAETGHLCLATLHANNANQALDRI
INFFPSEQHNQVWMDLSLNLKAIIAQQLVPTPDGKGRRAIIEILINTPLVSDLIRKAEVHKLKELMTKSGDVGMQTFDQA
LYQAYSAGEITYEDALAHADSANDLRLMIKLGANNSVANIQGGAGKLSLSDEQDYLPR

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1066256 ACH3PB_RS16350 WP_416396506.1 3644481..3645617(-) (pilU) [Allohahella sp. A8]
ATGGATTTCAACAAGCTTCTGAAGGTCATGGTTGAGAAGGGCGCGTCCGACCTGTTCATTACAGCCGGAGTGCCGCCCAG
CATGAAGGTCAACGGCCGCATGCTGCCAATCACAAAGACTTCGCTTTCGCCCGAAGAAACCCGCGAGATCGTGTTCTCGA
TCATGTCGGACAAGCAGCAGTCCGATTTCGAAGCGCATCATGAGTGCAACTTCGCGATCAGTGCCCGGGGAATTGGCCGG
TTCCGTGTGAGCGCGTTCTATCAGCGCAACCTCTGCGGCATGGTGTTGCGACGCATCGAAACCAATATCCCATCGGTCGA
CGATCTGAGGCTGCCAGAAACCATCAAGGAACTGGCTATGGCCAAGCGGGGACTCATCATCTTCGTGGGCGCGACCGGTA
CCGGTAAATCGACTTCGCTGGCAGCCATGATTGGCCACCGCAATGCGAACAGCAAAGGTCACATCATCTCCATCGAAGAC
CCGATCGAATTCATTCACCAGCACAGGGGCTGCATCGTTACGCAGCGTGAAGTAGGGCTTGATACTGAAAGCTTCGAAAT
GGCCTTGAAGAACACCCTGCGTCAGGCCCCCGACGTGATCATGATTGGCGAGGTGCGTAGTCCGGACACCATGGAGCATG
CTGTCGTGTTTGCGGAAACCGGTCACCTCTGTCTGGCCACCTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGTATC
ATCAACTTCTTTCCGTCCGAGCAGCACAATCAGGTGTGGATGGATCTCTCGCTCAACCTGAAGGCGATTATAGCGCAGCA
GCTCGTACCGACGCCTGATGGCAAGGGACGACGTGCGATCATAGAAATTTTGATCAATACACCCTTGGTCTCTGATCTTA
TCCGCAAGGCCGAAGTACACAAGCTGAAAGAGCTGATGACCAAGTCCGGCGACGTAGGCATGCAGACCTTCGATCAGGCG
CTGTATCAGGCTTACAGTGCTGGCGAGATTACCTACGAAGATGCGCTGGCTCATGCCGACTCAGCCAATGACCTCCGCCT
GATGATAAAACTGGGCGCCAATAATAGCGTGGCGAATATCCAGGGTGGCGCTGGCAAGCTGTCACTCAGTGATGAGCAGG
ACTACCTGCCACGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.134

98.942

0.743
  pilU Acinetobacter baylyi ADP1

64.917

95.767

0.622
  pilU Vibrio cholerae strain A1552

57.429

92.593

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.918

96.561

0.405

Multiple sequence alignment