Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACHZ97_RS15570 Genome accession   NZ_CP172310
Coordinates   3303309..3304346 (-) Length   345 a.a.
NCBI ID   WP_396615647.1    Uniprot ID   -
Organism   Lysobacter soli strain P18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3298309..3309346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHZ97_RS15560 (ACHZ97_15560) - 3299629..3301902 (+) 2274 WP_396615645.1 ATP-binding protein -
  ACHZ97_RS15565 (ACHZ97_15565) pilU 3301983..3303149 (-) 1167 WP_396615646.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHZ97_RS15570 (ACHZ97_15570) pilT 3303309..3304346 (-) 1038 WP_396615647.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHZ97_RS15575 (ACHZ97_15575) - 3304457..3305140 (+) 684 WP_396615648.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHZ97_RS15580 (ACHZ97_15580) proC 3305178..3306014 (+) 837 WP_396615649.1 pyrroline-5-carboxylate reductase -
  ACHZ97_RS15585 (ACHZ97_15585) - 3306027..3306539 (+) 513 WP_396615650.1 DUF4426 domain-containing protein -
  ACHZ97_RS15590 (ACHZ97_15590) - 3306514..3306858 (-) 345 WP_396615651.1 hypothetical protein -
  ACHZ97_RS15595 (ACHZ97_15595) - 3306855..3307658 (-) 804 WP_396615652.1 cobalamin-binding protein -
  ACHZ97_RS15600 (ACHZ97_15600) aac(6') 3307655..3308113 (-) 459 WP_396615653.1 aminoglycoside 6'-N-acetyltransferase -
  ACHZ97_RS15605 (ACHZ97_15605) pdxH 3308154..3308756 (-) 603 WP_157030971.1 pyridoxamine 5'-phosphate oxidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38518.32 Da        Isoelectric Point: 6.7269

>NTDB_id=1066224 ACHZ97_RS15570 WP_396615647.1 3303309..3304346(-) (pilT) [Lysobacter soli strain P18]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLDDLGCPPIFRELIQQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAGWEIMVGIPAIRNLIREDKVAQMYSAIQTGQSYGMMTLDQHL
QDLVKRGLILRPQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1066224 ACHZ97_RS15570 WP_396615647.1 3303309..3304346(-) (pilT) [Lysobacter soli strain P18]
ATGGATATCGCCGAACTTCTGGCGTTCTCGGTCAAGAACAAAGCATCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCGGACTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTATTCCGTACGATTCCGTCGGAAGTGCTCACGCTCGA
CGACCTGGGCTGCCCGCCGATCTTCCGCGAGCTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCATATCCTCTCCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGACCCCGACTACATCCTCGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCGGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCCCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTGGGCGGCGGGCGTACGGCGGGCTGGGAAATCATGGTCGGCATTCCGGCCATCCGTAACCTCATCC
GCGAAGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGAGCTACGGAATGATGACGCTCGACCAGCACTTG
CAGGACCTCGTCAAGCGCGGCCTGATCCTGCGACCGCAGGCGAAGGAATACGCGAAGGACAAGCGGTTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.213

95.362

0.67
  pilT Vibrio cholerae strain A1552

70.213

95.362

0.67
  pilT Neisseria meningitidis 8013

65.103

98.841

0.643
  pilT Neisseria gonorrhoeae MS11

64.62

99.13

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.909

95.652

0.391
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38

Multiple sequence alignment