Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACF0KO_RS00495 Genome accession   NZ_CP172307
Coordinates   108666..109703 (-) Length   345 a.a.
NCBI ID   WP_064026984.1    Uniprot ID   A0A1S1XZW6
Organism   Methylomonas sp. 2B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103666..114703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0KO_RS00475 (ACF0KO_00475) - 103979..105499 (-) 1521 WP_064026995.1 fumarate hydratase -
  ACF0KO_RS00480 (ACF0KO_00480) - 105711..105923 (-) 213 WP_064026993.1 hypothetical protein -
  ACF0KO_RS00485 (ACF0KO_00485) mltB 106332..107498 (-) 1167 WP_064026989.1 lytic murein transglycosylase B -
  ACF0KO_RS00490 (ACF0KO_00490) pilU 107517..108650 (-) 1134 WP_064026987.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACF0KO_RS00495 (ACF0KO_00495) pilT 108666..109703 (-) 1038 WP_064026984.1 type IV pilus twitching motility protein PilT Machinery gene
  ACF0KO_RS00500 (ACF0KO_00500) - 109942..110274 (-) 333 WP_064026982.1 TraR/DksA family transcriptional regulator -
  ACF0KO_RS00505 (ACF0KO_00505) - 110405..111310 (+) 906 WP_064026980.1 MoxR family ATPase -
  ACF0KO_RS00510 (ACF0KO_00510) - 111307..112251 (+) 945 WP_064026978.1 DUF58 domain-containing protein -
  ACF0KO_RS00515 (ACF0KO_00515) - 112248..114194 (+) 1947 WP_064026976.1 DUF3488 and transglutaminase-like domain-containing protein -
  ACF0KO_RS00520 (ACF0KO_00520) - 114215..114520 (-) 306 WP_064026974.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38280.17 Da        Isoelectric Point: 7.0361

>NTDB_id=1066189 ACF0KO_RS00495 WP_064026984.1 108666..109703(-) (pilT) [Methylomonas sp. 2B]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPPLNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAAGVFRTIPSKVLTLEDLDAPKFFAELCTKPRGLILVTGPTGSGKSTTLAAMINHINTNDYAHILTVED
PIEFVHESQKSLINQREIHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKAAMTRAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1066189 ACF0KO_RS00495 WP_064026984.1 108666..109703(-) (pilT) [Methylomonas sp. 2B]
ATGGACATCGCAGAATTGCTGACTTTTTCCGTTAAAAACAAGGCCTCGGACCTGCACTTGTCCGCCGGCTTGCCGCCGAT
GATCCGGGTGGACGGCGACATCCGGCGGATCAACATCCCGCCGCTCAATCACAAGGAAGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCCGGGATTACGAAGAGTTTCTGGAGACAGACTTTTCGTTCGCGCTGCCTGGCGTGGCGCGG
TTCCGGGTTAACGCCTTCAATCAGGATCGCGGCGCGGCCGGCGTGTTCCGGACCATTCCGTCCAAGGTGCTGACGTTGGA
AGATTTGGACGCGCCGAAATTTTTCGCGGAACTGTGTACCAAACCACGCGGCTTGATCCTGGTGACCGGACCGACCGGTT
CCGGTAAGTCGACGACGCTAGCGGCGATGATCAATCACATCAACACCAACGATTACGCGCACATCCTGACCGTCGAAGAC
CCGATCGAATTCGTTCACGAAAGCCAGAAATCGCTGATCAACCAACGCGAAATTCACCGCGATACCTTGGGTTTTAACGA
AGCCTTGCGCTCGGCCTTGCGGGAAGACCCCGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGGCTGG
CGCTGACCGCCGCGGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCACCTCGGCCGCGAAAACCATAGACCGGATT
ATCGACGTGTTCCCCGCCGCCGAGAAAGACATGATCCGGGCCATGCTGTCGGAATCCTTGCAAGCGGTGATTTCTCAAAC
GCTGCTGAAGAAAGTCGGCGGCGGCCGAATCGCCGCGCACGAAATCATGGTCGGCACGCCGGCGATTCGCAACCTGATCC
GCGAGGCCAAGGTCGCGCAAATGTATTCGGCGATCCAAACCGGCCGCAAGGACGGCATGCAGACGCTGGATCAAAACCTG
AAGGAACTGGTTGATAAGGGTTTGATCACCGCGAAGGCGGCGATGACGCGCGCGGTCAACAAAGATATGTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S1XZW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

79.472

98.841

0.786
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Legionella pneumophila strain Lp02

72.965

99.71

0.728
  pilT Legionella pneumophila strain ERS1305867

72.965

99.71

0.728
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.681

0.67
  pilT Vibrio cholerae strain A1552

68.546

97.681

0.67
  pilT Neisseria gonorrhoeae MS11

67.055

99.42

0.667
  pilT Neisseria meningitidis 8013

67.055

99.42

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Acinetobacter baylyi ADP1

40.058

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4
  pilU Vibrio cholerae strain A1552

38.938

98.261

0.383

Multiple sequence alignment