Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACF0KO_RS00490 Genome accession   NZ_CP172307
Coordinates   107517..108650 (-) Length   377 a.a.
NCBI ID   WP_064026987.1    Uniprot ID   A0A1S1XZT8
Organism   Methylomonas sp. 2B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 102517..113650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0KO_RS00475 (ACF0KO_00475) - 103979..105499 (-) 1521 WP_064026995.1 fumarate hydratase -
  ACF0KO_RS00480 (ACF0KO_00480) - 105711..105923 (-) 213 WP_064026993.1 hypothetical protein -
  ACF0KO_RS00485 (ACF0KO_00485) mltB 106332..107498 (-) 1167 WP_064026989.1 lytic murein transglycosylase B -
  ACF0KO_RS00490 (ACF0KO_00490) pilU 107517..108650 (-) 1134 WP_064026987.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACF0KO_RS00495 (ACF0KO_00495) pilT 108666..109703 (-) 1038 WP_064026984.1 type IV pilus twitching motility protein PilT Machinery gene
  ACF0KO_RS00500 (ACF0KO_00500) - 109942..110274 (-) 333 WP_064026982.1 TraR/DksA family transcriptional regulator -
  ACF0KO_RS00505 (ACF0KO_00505) - 110405..111310 (+) 906 WP_064026980.1 MoxR family ATPase -
  ACF0KO_RS00510 (ACF0KO_00510) - 111307..112251 (+) 945 WP_064026978.1 DUF58 domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42297.64 Da        Isoelectric Point: 6.4434

>NTDB_id=1066188 ACF0KO_RS00490 WP_064026987.1 107517..108650(-) (pilU) [Methylomonas sp. 2B]
MDFKALLALMVEKRASDLFITAGRAPSMKVDGKVVEVSKGVLNSEQTMKLVLSVMSQRQRDEFENTKECQFALSVSELGR
FRVSAFTQRDSAGMVLRRIETTIPTAEQLHLPPVLKDLIMYKRGLVMFVGATGTGKSTSLAALIRHRNENTSGHIISIED
PIEFIHPHQGCIVTQREVGLDTESFEVALKNTLRQAPDVILIGEVRTRDTMQHAITFAETGHLCVCTLHANNANQALDRI
LHFFPEEMHSQLFMDLSLNLRGIVAQQLVQRSDGKGRYPAIEILLNTPLVSDMIRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYAAGKISYEDALHAADSRNEVRLMIKLGAENVNFEVKDMMLEENDDDGGALYK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1066188 ACF0KO_RS00490 WP_064026987.1 107517..108650(-) (pilU) [Methylomonas sp. 2B]
ATGGACTTTAAAGCCTTATTGGCGCTGATGGTCGAGAAAAGAGCGTCCGACCTGTTCATTACCGCCGGCCGGGCGCCGTC
GATGAAAGTGGACGGCAAGGTCGTCGAAGTATCCAAGGGTGTTTTGAACAGCGAACAAACCATGAAACTGGTGCTCAGCG
TGATGTCGCAACGCCAGCGCGACGAGTTCGAGAATACCAAGGAATGCCAGTTCGCGCTGAGTGTCAGTGAATTGGGCCGC
TTCCGGGTAAGCGCCTTTACTCAACGCGATTCGGCCGGCATGGTGTTACGCCGCATCGAAACCACGATTCCGACCGCCGA
ACAACTGCACCTGCCGCCGGTGCTGAAAGACTTGATCATGTATAAGCGCGGTCTGGTGATGTTCGTCGGCGCGACCGGCA
CCGGTAAATCCACATCGCTGGCGGCGCTGATTAGGCATCGTAACGAAAACACCAGCGGCCACATCATTTCGATCGAAGAC
CCGATCGAGTTCATCCATCCGCACCAAGGCTGTATTGTGACGCAACGCGAAGTGGGCCTGGATACCGAGTCCTTCGAAGT
CGCGCTGAAAAACACCTTGCGGCAAGCGCCCGACGTGATCCTGATCGGCGAGGTACGCACCCGCGACACGATGCAGCACG
CCATCACCTTCGCCGAAACCGGTCACTTGTGCGTTTGTACCTTGCATGCCAACAACGCCAACCAAGCGTTGGACCGGATT
CTGCATTTCTTTCCGGAGGAGATGCATAGCCAATTGTTCATGGACCTATCGTTGAATCTGCGTGGTATCGTCGCTCAACA
ACTGGTGCAACGCTCTGACGGCAAGGGCCGCTATCCGGCGATCGAAATTCTGTTGAACACCCCGCTGGTGTCGGACATGA
TCCGCAAGGGCGAAGTGCATAAACTGAAGGAATTGATGAAGAATTCGCGCGAACACGGCATGCAGACTTTCGACCAAGCG
CTTTACGATTTGTACGCAGCCGGTAAAATTAGCTACGAAGACGCGCTGCACGCCGCCGACTCCCGCAACGAAGTCCGCCT
GATGATCAAACTGGGCGCCGAAAACGTCAATTTCGAGGTCAAGGACATGATGCTGGAAGAAAACGACGACGACGGAGGCG
CGCTGTATAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S1XZT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.929

100

0.676
  pilU Acinetobacter baylyi ADP1

62.254

94.164

0.586
  pilU Vibrio cholerae strain A1552

56.857

92.838

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.103

92.308

0.398
  pilT Legionella pneumophila strain ERS1305867

41.298

89.92

0.371
  pilT Legionella pneumophila strain Lp02

41.298

89.92

0.371
  pilT Acinetobacter baumannii D1279779

40.708

89.92

0.366
  pilT Acinetobacter baumannii strain A118

40.708

89.92

0.366
  pilT Acinetobacter nosocomialis M2

40.413

89.92

0.363
  pilT Pseudomonas aeruginosa PAK

40.413

89.92

0.363
  pilT Acinetobacter baylyi ADP1

39.883

90.451

0.361

Multiple sequence alignment