Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFWHB_RS18545 Genome accession   NZ_CP172298
Coordinates   4050914..4051948 (+) Length   344 a.a.
NCBI ID   WP_111912129.1    Uniprot ID   A0AAW9YFR4
Organism   Aeromonas sp. A-8     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4045914..4056948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFWHB_RS18505 (ACFWHB_18505) - 4046089..4046463 (+) 375 WP_025325672.1 YacL family protein -
  ACFWHB_RS18510 (ACFWHB_18510) - 4046514..4046810 (+) 297 WP_005332293.1 hypothetical protein -
  ACFWHB_RS18515 (ACFWHB_18515) - 4047006..4047836 (+) 831 WP_395949015.1 lysostaphin resistance A-like protein -
  ACFWHB_RS18520 (ACFWHB_18520) - 4047915..4048334 (-) 420 WP_025325669.1 DUF4426 domain-containing protein -
  ACFWHB_RS18525 (ACFWHB_18525) yggU 4048339..4048647 (-) 309 WP_111912133.1 DUF167 family protein YggU -
  ACFWHB_RS18530 (ACFWHB_18530) - 4048647..4049198 (-) 552 WP_025325667.1 YggT family protein -
  ACFWHB_RS18535 (ACFWHB_18535) proC 4049222..4050046 (-) 825 WP_395949016.1 pyrroline-5-carboxylate reductase -
  ACFWHB_RS18540 (ACFWHB_18540) - 4050173..4050874 (-) 702 WP_395949017.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFWHB_RS18545 (ACFWHB_18545) pilT 4050914..4051948 (+) 1035 WP_111912129.1 type IVa pilus ATPase TapT Machinery gene
  ACFWHB_RS18550 (ACFWHB_18550) pilU 4051975..4053084 (+) 1110 WP_395949018.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFWHB_RS18555 (ACFWHB_18555) yaaA 4053117..4053890 (+) 774 WP_262113416.1 peroxide stress protein YaaA -
  ACFWHB_RS18560 (ACFWHB_18560) srmB 4054074..4055297 (-) 1224 WP_106885020.1 ATP-dependent RNA helicase SrmB -
  ACFWHB_RS18565 (ACFWHB_18565) - 4055426..4056136 (+) 711 WP_395949019.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38161.90 Da        Isoelectric Point: 6.7438

>NTDB_id=1066114 ACFWHB_RS18545 WP_111912129.1 4050914..4051948(+) (pilT) [Aeromonas sp. A-8]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQSRGSGAVFRTIPSTVLTLEDLGAPEMFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFANALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSIIQTGMTHGMQTMDQSL
KQLVNRGVVAALDAKAKAADPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1066114 ACFWHB_RS18545 WP_111912129.1 4050914..4051948(+) (pilT) [Aeromonas sp. A-8]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGTGAAGTACACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAGCTGGAGGAGAATTTCGAGGTGGACTTCTCCTTCGAGGTGCCCGGCATGGCGCGC
TTCCGGGTCAACGCCTTCCAGCAGTCGCGGGGCTCTGGCGCCGTGTTTCGTACCATACCGAGCACAGTCCTGACCCTGGA
AGATCTGGGGGCGCCGGAGATGTTTCGCAAGATAGCGGAGTTTCCCCGCGGCCTGGTGCTGGTCACGGGCCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTCGATTACATCAACGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTGCACGAGAACAAGCGCTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCAAGAGTTTCGCCAA
CGCCCTGCGCTCGGCCCTGCGTGAAGATCCGGACATCATACTGGTGGGGGAAATGCGCGATCTGGAGACCATACGCCTGG
CCATGACGGCGGCGGAGACGGGCCATCTGGTGTTCGGCACCCTGCACACCTCGTCGGCAGCCAAGACCATAGACAGGATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGAGATCCATGCTCTCCGAGTCCCTGCGGGCTGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGAATCCCGGCGGTGCGAAACCTGATCC
GGGAAGACAAGGTCGCCCAGCTCTACTCCATCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTCAACCGGGGCGTGGTTGCCGCCCTGGACGCCAAGGCCAAGGCGGCCGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

78.134

99.709

0.779
  pilT Pseudomonas stutzeri DSM 10701

77.616

100

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.163

100

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

73.636

95.93

0.706
  pilT Legionella pneumophila strain ERS1305867

73.636

95.93

0.706
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.632

99.419

0.523
  pilU Vibrio cholerae strain A1552

43.478

100

0.436
  pilU Pseudomonas stutzeri DSM 10701

41.449

100

0.416
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401

Multiple sequence alignment