Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFWHB_RS18550 Genome accession   NZ_CP172298
Coordinates   4051975..4053084 (+) Length   369 a.a.
NCBI ID   WP_395949018.1    Uniprot ID   -
Organism   Aeromonas sp. A-8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4046975..4058084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFWHB_RS18515 (ACFWHB_18515) - 4047006..4047836 (+) 831 WP_395949015.1 lysostaphin resistance A-like protein -
  ACFWHB_RS18520 (ACFWHB_18520) - 4047915..4048334 (-) 420 WP_025325669.1 DUF4426 domain-containing protein -
  ACFWHB_RS18525 (ACFWHB_18525) yggU 4048339..4048647 (-) 309 WP_111912133.1 DUF167 family protein YggU -
  ACFWHB_RS18530 (ACFWHB_18530) - 4048647..4049198 (-) 552 WP_025325667.1 YggT family protein -
  ACFWHB_RS18535 (ACFWHB_18535) proC 4049222..4050046 (-) 825 WP_395949016.1 pyrroline-5-carboxylate reductase -
  ACFWHB_RS18540 (ACFWHB_18540) - 4050173..4050874 (-) 702 WP_395949017.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFWHB_RS18545 (ACFWHB_18545) pilT 4050914..4051948 (+) 1035 WP_111912129.1 type IVa pilus ATPase TapT Machinery gene
  ACFWHB_RS18550 (ACFWHB_18550) pilU 4051975..4053084 (+) 1110 WP_395949018.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFWHB_RS18555 (ACFWHB_18555) yaaA 4053117..4053890 (+) 774 WP_262113416.1 peroxide stress protein YaaA -
  ACFWHB_RS18560 (ACFWHB_18560) srmB 4054074..4055297 (-) 1224 WP_106885020.1 ATP-dependent RNA helicase SrmB -
  ACFWHB_RS18565 (ACFWHB_18565) - 4055426..4056136 (+) 711 WP_395949019.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACFWHB_RS18570 (ACFWHB_18570) brnQ 4056372..4057634 (+) 1263 WP_234569036.1 branched-chain amino acid transport system II carrier protein -
  ACFWHB_RS18575 (ACFWHB_18575) - 4057723..4057950 (-) 228 WP_163135628.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40871.99 Da        Isoelectric Point: 6.3874

>NTDB_id=1066115 ACFWHB_RS18550 WP_395949018.1 4051975..4053084(+) (pilU) [Aeromonas sp. A-8]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLAPLGGSPLDKQGALALVRESLDEAHFERYLHTREANYAIHREGLGR
FRVSAFWQQDLPGMVLRRIETRIPTFEELTLPLILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHGDGHILTVED
PVEFVHQHGRCLVTQREVGIDTESFNVALKNSMRQAPDVILIGEIRTQETMELAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLHGQRRVAAFEILLNTPLITDIVRKGEVHRLKEVMTKSGELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1066115 ACFWHB_RS18550 WP_395949018.1 4051975..4053084(+) (pilU) [Aeromonas sp. A-8]
ATGAACATGGATGTTCTGCTCGCTGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCTCCCAC
CCTCAAGGTCAACGGGCGTCTGGCGCCACTCGGGGGGAGCCCGCTGGACAAGCAGGGGGCACTGGCGCTGGTCAGGGAGA
GCCTGGACGAGGCGCACTTCGAGCGCTACCTGCACACCCGGGAGGCCAACTACGCCATTCACCGCGAGGGGCTGGGGCGA
TTTCGGGTCAGCGCCTTCTGGCAGCAGGATCTGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATTCCCACCTTCGA
GGAACTGACGCTGCCCCTCATACTCCAGGAGGTCGCCATGGCCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACAGGGG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTACCGCAACCAGCACGGCGACGGCCACATACTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCACCAGCATGGGCGCTGCCTGGTGACCCAGCGGGAGGTGGGGATAGACACCGAATCATTCAACGT
GGCCCTCAAGAATTCCATGCGCCAGGCGCCGGACGTCATCCTCATAGGCGAGATCCGCACCCAGGAGACCATGGAGCTGG
CCATCCAGTTTGCCGAGACGGGCCACCTCTGTCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAGCATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATAGTCGCACAGCA
ACTGCTGCCCTCCCTTCACGGGCAGCGCCGTGTCGCCGCCTTCGAGATACTGCTCAACACCCCGCTCATCACCGACATAG
TCCGCAAGGGAGAGGTGCACAGGCTCAAGGAGGTGATGACCAAATCCGGCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGTCGCGAGCAGCTGGGTGCAGGCACACTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

56.091

95.664

0.537
  pilT Legionella pneumophila strain ERS1305867

41.399

92.954

0.385
  pilT Legionella pneumophila strain Lp02

41.399

92.954

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.774

91.057

0.371
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366

Multiple sequence alignment