Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACHMXE_RS14270 Genome accession   NZ_CP172004
Coordinates   427768..428811 (-) Length   347 a.a.
NCBI ID   WP_203203102.1    Uniprot ID   -
Organism   Variovorax sp. UC122_21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422768..433811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXE_RS14255 (ACHMXE_14270) - 423544..424452 (-) 909 WP_395349824.1 NAD(P)-dependent oxidoreductase -
  ACHMXE_RS14260 (ACHMXE_14275) - 424557..426590 (+) 2034 WP_395349825.1 MFS transporter -
  ACHMXE_RS14265 (ACHMXE_14280) pilU 426606..427742 (-) 1137 WP_203203103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHMXE_RS14270 (ACHMXE_14285) pilT 427768..428811 (-) 1044 WP_203203102.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHMXE_RS14275 (ACHMXE_14290) - 428863..429564 (+) 702 WP_395349829.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHMXE_RS14280 (ACHMXE_14295) - 429575..430042 (-) 468 WP_395349831.1 Lrp/AsnC ligand binding domain-containing protein -
  ACHMXE_RS14285 (ACHMXE_14300) putA 430155..433131 (+) 2977 Protein_423 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA -
  ACHMXE_RS14290 (ACHMXE_14305) - 433147..433626 (-) 480 WP_209542897.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38235.97 Da        Isoelectric Point: 6.8885

>NTDB_id=1064768 ACHMXE_RS14270 WP_203203102.1 427768..428811(-) (pilT) [Variovorax sp. UC122_21]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGMGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTGQGQGMQTLDQN
LTDLVRRNTISAAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1064768 ACHMXE_RS14270 WP_203203102.1 427768..428811(-) (pilT) [Variovorax sp. UC122_21]
GTGGACATCACCCAACTGCTCGCGTTCAGCGTCAAGAACAAGGCTTCCGACCTGCATCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTTCACGGCGATGTGCGCCGCATCAATGTCGATGCGCTGGACCACAAGGCGGTGCACGCCATGGTCTACGACA
TCATGAGCGACACCCATCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGGCGCGCGGGGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTGAATGCGCCCAAGATTTTCGGCGAACTCGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGTCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGATCAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTCGAGGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACCCTGTCGTTCTCGAA
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCCGACGCCATCCTGGTCGGCGAGTTGCGCGACCTCGAGACCATCCGCCTGG
CGATGACCGCGGCCGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAGAAGGAGATGATCCGCGCCATGCTGTCGGAGTCGCTGCAGGCGGTGATCTCGCAGAC
CCTGTGCAAGACCAAGGACGGCATGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCTCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCCAGGGCATGCAGACGCTGGACCAGAAC
CTCACGGACCTGGTGCGGCGCAATACGATCTCCGCCGCGGAAGCCCGCAGCAAGGCCAAGATCCCCGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Vibrio cholerae strain A1552

68.622

98.271

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.622

98.271

0.674
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment