Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHMXE_RS14265 Genome accession   NZ_CP172004
Coordinates   426606..427742 (-) Length   378 a.a.
NCBI ID   WP_203203103.1    Uniprot ID   -
Organism   Variovorax sp. UC122_21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421606..432742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXE_RS14245 (ACHMXE_14260) - 421844..422488 (+) 645 WP_395349823.1 SIS domain-containing protein -
  ACHMXE_RS14250 (ACHMXE_14265) - 422685..423476 (+) 792 WP_281080937.1 BON domain-containing protein -
  ACHMXE_RS14255 (ACHMXE_14270) - 423544..424452 (-) 909 WP_395349824.1 NAD(P)-dependent oxidoreductase -
  ACHMXE_RS14260 (ACHMXE_14275) - 424557..426590 (+) 2034 WP_395349825.1 MFS transporter -
  ACHMXE_RS14265 (ACHMXE_14280) pilU 426606..427742 (-) 1137 WP_203203103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHMXE_RS14270 (ACHMXE_14285) pilT 427768..428811 (-) 1044 WP_203203102.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHMXE_RS14275 (ACHMXE_14290) - 428863..429564 (+) 702 WP_395349829.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHMXE_RS14280 (ACHMXE_14295) - 429575..430042 (-) 468 WP_395349831.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41823.96 Da        Isoelectric Point: 6.2427

>NTDB_id=1064767 ACHMXE_RS14265 WP_203203103.1 426606..427742(-) (pilU) [Variovorax sp. UC122_21]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDDLGMPQVLKDVSMTKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEAHAISFEDALRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1064767 ACHMXE_RS14265 WP_203203103.1 426606..427742(-) (pilU) [Variovorax sp. UC122_21]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCCGACTTCCCGCCCGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTGATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGACCTGGGCATGCCGCAGGTGCTCAAGGACGTGTCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAACTCCTTCGGC
CACATCGTCACGGTGGAGGACCCGGTGGAGTTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGCAT
CGACACCGACAGCTGGGAGGCGGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCGGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCGCGCAGCTCCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTCTCGCAGCGCCTGGTGCCGACCGAGGACGGCCTGGGCCGCGTGGCGGCGGTCGAGATCCTGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGCCCATGCGATCAGCTTCGAGGATGCGCTGCGCAATGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCCGCGGGCACGGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373

Multiple sequence alignment